Sentences with phrase «in aliquots»

Stock solution (10 mg / mL) was prepared in DMSO and stored in aliquots at — 20 °C.

Not exact matches

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients.

Two aliquots each of purified plasmid DNA's containing envelope sequence subtype refrenceThe plasmids in this kit are for use in HMA only.

Samples are stored under highly controlled conditions and, if appropriate, distributed in several aliquots to avoid thawing and refreezing.

Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].

An aliquot of coded plasma from the CFS and healthy controls was tested at RKI by an ELISA using recombinant Gag and Env proteins used recently to investigate XMRV infection in German prostate cancer patients [14].

Each organ was weighed, either the whole or partial organ was homogenized in 1 ml PBS, 100 - μl aliquots (in triplicate) were counted, and the total organ uptake was calculated.

Interestingly, in the current study, we were unable to recover sequence from this related MLV in a fresh aliquot of 2003 LnCaP cells by deep sequencing, and instead, found only XMRV (Fig. 5; Fig.

In brief, six aliquots of DNA from each specimen were tested for DYS14.

A 100 - μl aliquot of the buffer was used for the lipase assay in combination with 100 μl of a 10 % Intralipid / [3H] triolein emulsion for 1 hour at 25 °C.

These aliquots were used to initiate blood infections in naïve mice that were then used as donors of asexual parasites to mice involved in the posttransmission experiments.

The luminescence dating of the Monte Verde sediments was based on the Single - Aliquot Regenerative (SAR) dose protocol [40](see Text G in S1 File) applied to concentrates of quartz and K - rich feldspar in the 180 — 250 μm grain size.

Samples in which only 1 or 2 of the triplicate aliquots were positive could only be considered presumptively positive.

Aliquots of the 38 plasma samples collected during each 24 - h GCRC admission were pooled as described in Subjects and Methods for measurement of average hourly plasma ghrelin concentrations.

(d) In addition, if the test fails to reconfirm the presence of the drug (s) / drug metabolite (s) reported in the primary specimen, you may send the specimen or an aliquot of it for testing at another HHS - certified laboratory that has the capability to conduct another reconfirmation tesIn addition, if the test fails to reconfirm the presence of the drug (s) / drug metabolite (s) reported in the primary specimen, you may send the specimen or an aliquot of it for testing at another HHS - certified laboratory that has the capability to conduct another reconfirmation tesin the primary specimen, you may send the specimen or an aliquot of it for testing at another HHS - certified laboratory that has the capability to conduct another reconfirmation test.

(b) In addition, if the test fails to reconfirm the adulterant result reported in the primary specimen, you may send the specimen or an aliquot of it for testing at another HHS - certified laboratory that has the capability to conduct another reconfirmation tesIn addition, if the test fails to reconfirm the adulterant result reported in the primary specimen, you may send the specimen or an aliquot of it for testing at another HHS - certified laboratory that has the capability to conduct another reconfirmation tesin the primary specimen, you may send the specimen or an aliquot of it for testing at another HHS - certified laboratory that has the capability to conduct another reconfirmation test.

The medication they received from their veterinarian was not dispensed in the CVM - approved container but was broken into aliquots that were taken home without the client information sheet or approved label.

Helped design and carry out experimental procedures Performed Immunochemical staining Prepared Media and aliquots Conducted basic biological manipulations of bacteria Performed cell culture and seeding Participated in the Department's Quality Control, Quality Assurance, Safety, and preventive maintenance programs.