Not exact matches
Another issue he tackled was anisotropy, that is, the fact that a single objective will typically give better
resolution in the lateral xy direction than
in the
axial z direction.
As explained
in the research paper, the
resolution approaches the angstrom scale only
in the
axial direction, where it is estimated at 0.6 nm.
While STED can overcome the diffraction barrier down to 5 nm
in the lateral direction, 4Pi Microscopy can increase the
resolution in the
axial direction by using two opposing lenses.
In The Optical Society (OSA) journal Optics Express, the researchers show that their new approach can create microstructures with a 1.0 - micron lateral (side - to - side) and 21.5 - micron
axial (depth) printing
resolution.
It is ideal for studying events
in the plasma membrane of living cells with high
axial resolution.