Sentences with phrase «in blastomeres»

These findings on gene expression in single embryonic stem cells are in concert with recent studies of early mammalian development, which reveal molecular heterogeneity and a stochasticity of gene expression in blastomeres.

Not exact matches

More than half of the 91 blastomeres divided at least once, and 28 formed clumps that grew in culture.

In Xenopus, Noggin can direct the progeny of early blastomeres, not normally destined to contribute to the eyes, to a retinal fate [19], [20].

From our experience with Preimplantation Genetic Diagnosis (PGD) we saw that a low mutation load at the blastomere stage reflects the mutation load in the child when it is born.

This interpretation is supported by a previous study showing that the blastomeres from arrested human embryos still divided in vitro when isolated from the original embryos [42].

The results indicate the potential for individual blastomere growth and development from parthenotes obtained from in vitro maturation in spite of the fact that we did not derive a pESC line at this time.

In our study, cleaved day 3 parthenotes were used to establish pESCs from single blastomeres using derivation methods as previously described [41].

Only 18.8 % of in vivo - derived embryos contained at least one blastomere with chromosomal anomalies, compared to 69.2 % of OPU - IVF embryos (P < 0.01) and 84.6 % of IVM - IVF embryos (P < 0.001).

Likewise, the expression pattern of cdx - 2, a key transcription factor in the trophoblast lineage, overlaps with that of Oct - 4, but within any blastomere, cdx - 2 expression bears no consistent relationship to that of Oct - 4 or nanog until after the trophectoderm lineage has been sorted [9].

Subsequently, genome - wide haplotyping and copy - number profiling was applied to investigate the genomic architecture of 171 single bovine blastomeres of 16 in vivo, 13 OPU - IVF and 13 IVM - IVF embryos.

Main results and the role of chance: The genomic stability of single blastomeres in both of the in vitro - cultured embryo cohorts was severely compromised (P < 0.0001), and the frequency of whole chromosome or segmental aberrations was higher in embryos produced in vitro than in embryos derived in vivo.