Sentences with phrase «in bleomycin»

b) Graph showing significant downregulation of CD44 in Bleomycin treated siTRF2 silenced HCT116 cells as compared to Bleomycin treated scrambled transfected cells (p < 0.05).

Treatment with plerixafor prevents pulmonary fibrosis in bleomycin - induced murine pulmonary fibrosis.

Not exact matches

In his book It «sNot About the Bike, Armstrong recalls how, on that grim morning, the oncologistoutlined a treatment protocol involving the drug bleomycin, which would sodamage his lungs that he would not be able to race again.

In mice given bleomycin, the researchers found that the levels of CHI3L1 declined at first and then surged.

d) Graph showing higher frequency of γH2Ax foci / nuclei at 40 and 80 μM dose of Bleomycin post silencing in siTRF2 silenced cells compared to Scrambled transfected HCT - 116 cells (p < 0.05).

N - acetylcysteine amide, a thiol antioxidant, prevents bleomycin - induced toxicity in human alveolar basal epithelial cells (A549).

d) Immunocytochemistry analysis showing reduced CD44 expression in siTRF2 silenced HCT116 post treatment with Bleomycin sulphate when compared to scrambled transfected HCT116 post treatment with Bleomycin.

Mean γH2Ax foci / nuclei was observed to significantly increase (p < 0.05) with increasing concentrations of Bleomycin (20, 40, 60, 80 μM) in parental HCT - 116 (Fig 1e and 1f).

Unpaired student's t - test was also used to compare gene expression variation in siTRF2 silenced versus non silenced scrambled siRNA HCT116 cells in the presence of bleomycin sulfate.

c) Graph showing dose dependant (20, 40, 60, 80 µM) increase in CD44 enriched cells post treatment with Bleomycin sulphate (p < 0.001).

Furthermore, on treatment with 80μM bleomycin, a significant (p < 0.05) increase in expression of stemness genes like CD44, Oct 4 and a significant downregulation of CD24 gene (p < 0.05) was observed in clonospheric HCT116 when compared to Parental HCT116 (Fig. 3a and 3b).

d) Graph showing quantitative average olive tail moment in parental and clonospheres post DNA damage with 40 and 80 μM of Bleomycin.

To analyse the extent of DNA damage response (DDR) in resistant phenotype, we treated spheroidal clonospheres with specific concentrations of Bleomycin.

With increasing dosage of Bleomycin, significantly low foci per nuclei (p < 0.05) was observed in clonospheres as compared to that in parental HCT - 116 (Fig 2a and 2b).

We report the presence of cancer stem cell like Side Population and their survival and enrichment post treatment with bleomycin in HCT116 cell line.

Comparative gene expression analysis revealed a significant overexpression of TRF2, β - catenin and hTERT genes (p < 0.01) in clonospheres at high Bleomycin concentration (80μM)(Fig 2f).

Chen J, Ghorai MK, Kenney G, Stubbe J. Mechanistic studies on bleomycin - mediated DNA damage: multiple binding modes can result in double - stranded DNA cleavage.

Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects.

Immunocytochemistry also confirmed reduced expression of CD44 in siTRF2 silenced HCT116 cells as compared to Scrambled siRNA when treated with increasing concentration of Bleomycin (Fig 4d).

To further characterize the stem cell like properties of SP cells we treated HCT116 with increasing concentrations of Bleomycin and observed variation in CD44 percentage.

A significant increase in stem cells in terms of CD44 positive population was observed with higher Bleomycin concentration (p < 0.001)(Fig 1c and 1d).

Elevated TRF2 expression in clonospheres at high concentration of Bleomycin may contribute to increased expression of stem cell factors.

DNA damage, in terms of average olive tail moment, was observed to increase with increasing concentration of Bleomycin in Parental HCT 116 cells.

Knockout of endothelin type B receptor signaling attenuates bleomycin - induced skin sclerosis in mice.