These results provide the validity to the newly developed hypoxia / reoxygenation culture system for examining the regulation of
CSCs in breast cancer cell lines by niche factors in the tumor microenvironment and developing differential targeting strategies to eradicate breast CSCs.
The researchers studied the two
proteins in breast cancer cell lines, where they discovered that TDRD3 recruits TOP3B to active chromatin (where genes are turned on) that is marked by arginine methylation.
Interestingly, TGFβ treatment increased both ALDEFLUOR ‐ positive cell number and tumorsphere
formation in a breast cancer cell line, whilst blocking of several TGFB1 ‐ related genes reduced bCSC number.