Sentences with phrase «in cell assays»

The Tissue Platform workgroup within autoimmune inflammatory diseases at Karolinska Institutet studies the effects of probes in cell assays relevant for the diseases we study, ie systemic lupus erythematosus, idiopathic inflammatory myositis, systemic sclerosis and inflammatory bowel disease.

Not exact matches

• NanoCellect Biomedical, a San Diego - based developer of microfluidic cell sorting technologies for cell - based assays, raised $ 10 million in series B funding.

The ability of SIF - seq to use reporter assays in mouse embryonic stem cells to identify human embryonic stem cell enhancers that are not present in the mouse genome opens the door to intriguing research possibilities as Dickel explains.

Richard Jones, M.D., professor and director of the Bone Marrow Transplant Program at Johns Hopkins, developed a now commonly used assay to study ALDH levels in individual cells.

Years ago W.U.'s Holtzman and former colleague Marc Diamond, who has since moved to the University of Texas Southwestern Medical Center, identified tau - binding antibodies that slow the spread of toxic tau clusters in a lab assay using cultured cells.

Cell assays and animal studies suggest that drugs that restore p53's activity work with not just one mutant form of the protein, but many, says Alan Fersht, a chemist at the University of Cambridge in the United Kingdom.

Stagljar and his colleagues also applied the new technology, which they dubbed MaMTH (for Mammalian - Membrane Two - Hybrid assay), to identify a protein that plays a role in the most common form of lung cancer called non-small cell lung cancer.

«There's a large amount of validation to ensure specificity of the antibodies and to make sure that the antibody is actually recognizing the intended target,» says Craig M. Thompson, director of production and molecular assays, with Cell Signaling Technology (CST), located in Danvers, Massachusetts.

In the new study, Bohn's group used cell signaling assays on a library of µ - receptor - activating compounds to find a few with hints of a bias toward G - protein signaling.

The ability to automate morphology assays on 3D cell cultures is a powerful tool, but some scientists are now pushing the technology even further, with algorithms that can classify structures in living animals and clinical specimens.

Every invaded cell in this assay is colored green and the assay showed that the cells on the left, lacking Id4, invaded much more than the cells on the right in which there was forced expression of Id4.

Invasion assays using Glioblastoma (GBM) cells on the left lacking Id4, in comparison to the same cells being genetically engineered to express Id4 on the right.

An antibody that works well for Western blots on denaturing gels, for example, should not be presumed to work equally well in nondenaturing assays such as fluorescence - activated cell sorting.

Testing the effectiveness of this molecule in their VLP assay, they found that it reduced the ability of the virus to bud off from human cells in culture by more than 90 percent and was similarly effective against proteins found in Ebola and HIV.

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

The assay can measure the lag times and growth rates of as many as 80,000 individual microcolonies in a single 24 - hour experiment, opening up a powerful new high - throughput tool to study the complex interplay between cell growth, division and metabolism under environmental conditions that are likely to be ecologically relevant but had previously been difficult to study in the laboratory.

We have been using cell culture models and zebrafish as an assay to determine the effects of BMP on signaling, but we also want to be able to understand what the effect of the mutation is in the tissue and organ systems.

A quantitative assay of the suppressor tRNA activity in these mammalian cells is described; it is based on the amount of tRNA - mediated readthrough of a terminating codon in the influenza virus NS1 gene after the cells are infected with virus.

This surface protein, which has not yet been named, also binds to normal PrP both in protein assays and in cell cultures.

In one of the assays, the cells with intact membranes produce a key enzyme, which indicates that they are alive.

Both assays (for translation enhancement and internal ribosomal initiation) were validated under cell - free conditions and in human cells, using a vaccinia virus vector.

They sent soil samples for DNA testing, looking for matches with particular genes known to be found in microbes and fungi; they tried to stimulate microbial growth on a wide variety of substances and then count the cells produced; and they used highly sensitive radiorespiration activity assays, which involve feeding the soil microorganisms a food source which has been labelled with radioactive carbon, which can then be used to detect if the microorganisms are active.

An example of these tools in our lab is the characterization and use of halide - sensitive variants of GFP to screen for activators and inhibitors of CFTR in cell - based assays.

Whereas in the past assays were predominantly in vitro using purified components, there is now a realization that cell - based, high - content assays are a more productive path.

Using genetic techniques, the Imperial team knocked out the Hhat function in pancreatic cancer cells and, as they hoped, the cancer cells showed substantially reduced growth and ability to spread in a test - tube assay.

Dr. Weston now leads a team of researchers in the design and implementation of high throughput, cell - based assays including high - content screening.

«The basic assays for repeat number, methylation status, and the number of uninterrupted repeats cost less than $ 5, the mark typically considered the threshold for population - based screening,» said Bruce Hayward, PhD, a Senior Research Fellow in the Laboratory of Cell and Molecular Biology and the report's first author.

Oxnard, working with researchers from the Belfer Institute for Applied Cancer Science at Dana - Farber, had recently developed an assay to detect and quantify EGFR mutations in cell - free DNA.

In vitro chondrogenesis assays demonstrated that the R206H ACVR1 mutation can stimulate progenitor cells to differentiate to cartilage in concert with the appropriate cell environment and differentiation factors and that this mutant receptor activates BMP signaling constitutively without BMP stimulatioIn vitro chondrogenesis assays demonstrated that the R206H ACVR1 mutation can stimulate progenitor cells to differentiate to cartilage in concert with the appropriate cell environment and differentiation factors and that this mutant receptor activates BMP signaling constitutively without BMP stimulatioin concert with the appropriate cell environment and differentiation factors and that this mutant receptor activates BMP signaling constitutively without BMP stimulation.

In this study, researchers identified one microRNA (MIR548K) encoded in this region, and found it was characterized as a novel oncogene and functional assays which demonstrated that MIR548K enhances malignant phenotypes of ESCC cellIn this study, researchers identified one microRNA (MIR548K) encoded in this region, and found it was characterized as a novel oncogene and functional assays which demonstrated that MIR548K enhances malignant phenotypes of ESCC cellin this region, and found it was characterized as a novel oncogene and functional assays which demonstrated that MIR548K enhances malignant phenotypes of ESCC cells.

Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.

The tiers range from computer - based approaches and cell assays to tests in fish and mammals.

Independently of Smads, BMP signaling also activates the p38 MAPK pathway (32 — 34); however, additional assays in COS - 7 cells showed no increase in p38 MAPK phosphorylation in response to ACVR1 (R206H)(data not shown).

However, chondrogenesis assays in chick micromass cultures showed that enhanced signaling by the mutant R206H ACVR1 can be partially inhibited by the BMP antagonist Noggin, suggesting that in addition to BMP - independent activity by the R206H ACVR1 receptor, this mutant receptor is also responsive to BMP, consistent with previous BMP signaling pathway studies that used cells derived from FOP patients (48 — 50).

C. Geminin / Ki67 assay to assess cell cycle arrest in vivo.

At 48 hours, cells were washed twice with PBS and lysed in 1 × passive lysis buffer (Dual - Luciferase Reporter Assay, Promega).

Next, because KLF4 is involved in self - renewal of stem cell, we also examined the effect of both miR - 7 and KLF4 on proliferation of CSCs by MTS assay and found that miR - 7 was capable of blocking the growth of CSCs, whereas ectopic expression of KLF4 significantly mitigated this effect of miR - 7 (Fig. 4C).

Using cell culture assays and genetically modified mice, we identified a number of signaling proteins that participate in muscle plasticity.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

The Molecular Screening and Protein Expression Shared Resource fosters collaboration by providing expertise in biochemical and cell - based assay development for high - throughput screening and compound profiling.

Once developed, the assays will be used to analyse in vitro cytokine and chemokine induction, e.g. to compare different versions of a vaccine, or the same vaccine produced in different host cells, or different batches of a vaccine to demonstrate batch - to - batch reproducibility.

We focus more specifically on: (A) GMP translations of CTP production and Quality Control assay development to evaluate such production (in collaboration with Roslin Cells, UK).

It is also pointed out that donor age (beyond 45 years in the alkaline phosphatase - linked BrdU assays) may also have limited the human wounding response (cell division) in the corneal periphery.

In order to determine whether cell division occurred in either the periphery of the corneal endothelium following mechanical wounding or at the site of the wound itself, corneas were tested with the AP - BrdU assaIn order to determine whether cell division occurred in either the periphery of the corneal endothelium following mechanical wounding or at the site of the wound itself, corneas were tested with the AP - BrdU assain either the periphery of the corneal endothelium following mechanical wounding or at the site of the wound itself, corneas were tested with the AP - BrdU assay.

The in vitro boosted effector cells were washed once with HBSS and then resuspended in CRPMI and used as effector cells in the cytotoxicity and apoptosis assays (below).

The role of DN T cells in corneal allograft rejection was confirmed in two separate in vitro assays in which CD8 − cells were isolated from CD4 KO donors that had rejected corneal allografts and were found to induce apoptosis of donor - specific corneal cells.

The assay will be validated against clinical outcomes in human vaccine studies (correlates of protection such as neutralising antibodies or vaccine - induced protection in human challenge models, or exploratory correlates such as multifunctional Th1 cells).

Importantly, researchers have the ability to carry out assays and screens directly in primary cells.

The presence of the male - specific SRY sequence was analyzed using a kinetic quantitative ELISA PCR assay that allows detection of one to three male cells in one million female cells.

The level of fluorescence you saw in the validation assay should give an accurate indication of how many pups will contain a mutation and many of the mice with the mutation will be homozygous for it in every cell; however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice.