Sentences with phrase «in cell culture experiments»

We also mimic these conditions on isolated precursor cells in cell culture experiments.

Those doses were chosen in analogy with the prion field, in which it is very common to observe that 1 % and 0.1 % tissue homogenate containing prion protein aggregates is able to induce pathological changes both in cell culture experiments and in in vivo animal models.

Not exact matches

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

While other papers have examined these mutations using expensive and time - consuming experiments on live ferrets and laboratory cell cultures, Deem and Melia Bonomo used the pEpitope method to rapidly calculate how much the egg - passage mutations would decrease vaccine efficacy in humans.

Shukla and colleagues discovered that a small drug molecule called BX795, which is sold to labs for use in experiments, helped clear HSV - 1 infection in cultured human corneal cells, in donated human corneas, and in the corneas of mice infected with HSV - 1.

Scientists are forced either to experiment on whole animals, which is expensive, raises ethical issues and may not predict effects in humans, or to perform tests on microscopic human cells found in tissue cultures, which have been altered to live forever and bear little relation to actual living, breathing people.

In a series of experiments, the researchers first identified a set of 19 transcription factors that were expressed at significantly greater levels in cultured human glioblastoma stem cells capable of tumor propagation than in differentiated tumor cellIn a series of experiments, the researchers first identified a set of 19 transcription factors that were expressed at significantly greater levels in cultured human glioblastoma stem cells capable of tumor propagation than in differentiated tumor cellin cultured human glioblastoma stem cells capable of tumor propagation than in differentiated tumor cellin differentiated tumor cells.

In experiments on cell cultures, both of these inhibitors succeeded in breaking various forms of the TKI resistance: including forms caused by additional mutations of the gene Bcr - Abl as well as those caused by large quantities of the protein GabIn experiments on cell cultures, both of these inhibitors succeeded in breaking various forms of the TKI resistance: including forms caused by additional mutations of the gene Bcr - Abl as well as those caused by large quantities of the protein Gabin breaking various forms of the TKI resistance: including forms caused by additional mutations of the gene Bcr - Abl as well as those caused by large quantities of the protein Gab2.

In other experiments, the protein blocked the virus from infecting cells in culture, and it even prevented mice from getting sick when administered either 1 day before or after viral exposure, they report today in Nature BiotechnologIn other experiments, the protein blocked the virus from infecting cells in culture, and it even prevented mice from getting sick when administered either 1 day before or after viral exposure, they report today in Nature Biotechnologin culture, and it even prevented mice from getting sick when administered either 1 day before or after viral exposure, they report today in Nature Biotechnologin Nature Biotechnology.

In her experiment, Wang starved cultured rat muscle cells in order to trigger apoptosis, a genetic program that instructs cells to self - destrucIn her experiment, Wang starved cultured rat muscle cells in order to trigger apoptosis, a genetic program that instructs cells to self - destrucin order to trigger apoptosis, a genetic program that instructs cells to self - destruct.

Writing in the May 4 online issue of the journal Scientific Reports, researchers at University of California, San Diego School of Medicine used a powerful statistical tool called «design of experiments» or DOE to determine the optimal cell culture formula to grow and produce hPSCs.

At the Galveston National Laboratory, which houses a biosafety level 4 lab, researchers shut down experiments, euthanized infected animals, put pathogens in cold storage, and autoclaved cell cultures.

In 2009, from experiments at the RIKEN Center for Developmental Biology in Kobe, Japan, he found that when culture conditions are right, adding a single ingredient — bone morphogenetic protein 4 (Bmp4)-- with precise timing is enough to convert embryonic cells to PGCIn 2009, from experiments at the RIKEN Center for Developmental Biology in Kobe, Japan, he found that when culture conditions are right, adding a single ingredient — bone morphogenetic protein 4 (Bmp4)-- with precise timing is enough to convert embryonic cells to PGCin Kobe, Japan, he found that when culture conditions are right, adding a single ingredient — bone morphogenetic protein 4 (Bmp4)-- with precise timing is enough to convert embryonic cells to PGCs.

After the pig experiment worked well, he used his Boston area facility to culture the 30 million cardiac muscle cells that he says were injected into a heart disease patient in mid-2011.

The researchers ran numerous experiments in cell cultures and mice to observe what happened to Salmonella in the inflamed gut when these genes were mutated.

Studies of IBD are typically performed using cell culture experiments or animal models, which don't mimic the precise conditions that occur in the gut of human patients.

«Then we cultured the cells in the presence of various drugs and screened for spontaneous drug - resistant mutants to isolate clones useful for symbiosis experiments.»

After experimenting with several different cocktails, the researchers hit on a mix that transformed 100 percent of cultured stem cells into those that looked like neurons in under an hour.

At the time, culturing stem cells was notoriously hard, but after combing through previous lab experiments, another postdoc in Clevers's lab, Toshiro Sato, concocted a mix of growth factors that coaxed the gut stem cells to replicate in a dish.

Set up experiments for whole week: Start cell cultures, book microscopes, make buffers, and even find time over the bacterial incubator for a spot of eyelash fluttering at the sexy postdoc in the lab next door.

The treatment did a lot to prevent cell migration, a key mechanism in metastasis, in experiments on laboratory cultures (in vitro) of cancerous human cells.

Together, Raman says, this dual - mode treatment produced cell - killing results that paralleled their experiments in cell cultures.

In the new research — published Oct. 21 in the early online edition of Proceedings of the National Academy of Sciences - Margeta, former postdoctoral associate Agata Habas, PhD, now at UC San Diego, and colleagues teased apart the relative contributions of neurons and astrocytes in Nrf2 signaling by performing experiments on cell cultures containing predominantly neurons, predominantly astrocytes, or a mixture of botIn the new research — published Oct. 21 in the early online edition of Proceedings of the National Academy of Sciences - Margeta, former postdoctoral associate Agata Habas, PhD, now at UC San Diego, and colleagues teased apart the relative contributions of neurons and astrocytes in Nrf2 signaling by performing experiments on cell cultures containing predominantly neurons, predominantly astrocytes, or a mixture of botin the early online edition of Proceedings of the National Academy of Sciences - Margeta, former postdoctoral associate Agata Habas, PhD, now at UC San Diego, and colleagues teased apart the relative contributions of neurons and astrocytes in Nrf2 signaling by performing experiments on cell cultures containing predominantly neurons, predominantly astrocytes, or a mixture of botin Nrf2 signaling by performing experiments on cell cultures containing predominantly neurons, predominantly astrocytes, or a mixture of both.

Brown University engineers have demonstrated a technique for making 3 - D - printed biomaterials that can degrade on demand, which can be useful in making intricately patterned microfluidic devices or in making cell cultures than can change dynamically during experiments.

The team conducted additional experiments in which mouse breast tumors were sampled to generate so - called organoids — spheres of cells that grow in three - dimensional culture.

The team's experiments in cell cultures, conducted with funding from an American Brain Tumor Association Discovery Grant, showed that coibamide A cuts off the cancer cells» ability to communicate with blood vessels and other cells, eventually starving the cell and triggering its death.

In order to determine which type of liver cells cause this accumulation, the researchers repeated the experiment using cultured hepatocytes.

A set of experiments in cultured bone cells confirmed that the human form of ER - a can restore the adaptive response to strain in cells where it is lacking.

Additional experiments with cultured cells suggested that the mutation interferes with one of the enzymes that breaks down the APP protein and causes a 40 % reduction in β amyloid formation, Stefansson and colleagues report online today in Nature.

Previous experiments from other labs have successfully brought embryos more than 10 days into development by implanting them on a three - dimensional scaffold of endometrial cells and nutrients in culture.

Preliminary experiments in cultured tumour cells confirm that the genomic instability present in these cells is partly caused by histone 1 deficiency.

This ability of NGF to sustain sympathetic neurons in culture has since made possible some ingenious and revealing experiments on neuronal differentiation [see «The Chemical Differentiation of Nerve Cells.»

These models include in vitro experiments with purified recombinant or synthetic proteins, cell culture experiments, and infectivity studies with Tg mice expressing the appropriate form of the protein.

In a first set of experiments, postdoctoral researcher and co-lead author Max - Felix Häring found that insulin increased the proliferation of mouse epithelial tumor cells in culture, as expecteIn a first set of experiments, postdoctoral researcher and co-lead author Max - Felix Häring found that insulin increased the proliferation of mouse epithelial tumor cells in culture, as expectein culture, as expected.

Using cell culture and laboratory mouse experiments, the researchers showed that the enzyme, aldose reductase, is essential to a process known as goblet cell metaplasia that is seen in both asthma and COPD.

«In cell culture, this structure does not exist, and thus there are inherent limitations to what types of experiments can be performed and what conclusions can be drawn.»

And sure enough, this increased burden of senescent cells caused the same problems in living mice as in the researchers» cell culture experiments, as well as in previous work in paraquat - exposed mice.

The idea for a spinoff medical technology company originated several years ago when Medlin and his adviser, Endre Takacs, were working in Clemson's Atomic and Medical Physics lab on an experiment with bioengineers to systematically study the effects of low - dose radiation on living cell cultures.

In this course, trainees will perform experiments involving both classical physiological models, as well as modern reductionist approaches and confocal microscopy to follow trafficking of transporter proteins in cultured cellIn this course, trainees will perform experiments involving both classical physiological models, as well as modern reductionist approaches and confocal microscopy to follow trafficking of transporter proteins in cultured cellin cultured cells.

For lethal toxin experiments MNK - 3 cells were cultured in media containing 10 ng / ml IL - 7.

This ruling allows researchers to continue feeding embryonic stem cell cultures, experimenting with mice, and other research activities until this court rules, the U.S. Supreme Court weighs in, or Congress passes legislation that clarifies the issues.

(A, B) Neurite length of neurons in E14 rat VM cultures transfected with control siRNA, Smad4 siRNA or Smad1 siRNA or Smad5 siRNA and a GFP - expressing plasmid before being cultured with or without 10 ng / ml GDF5 for 72 h (*** P < 0.001 compared with control; ANOVA with post-hoc Tukey's test; 40 cells for each group per experiment; number of repetitions (n) = 3 experiments).

The above serial cell passaging and replating experiments show that the PC3 holoclones contain self - renewing cancer cells or CSCs that can sustain long - term propagation in culture.

However, it is practically difficult to perform the rescue experiments in the organoid culture system because the lentivirus transfection is required for the gene transduction into the primary stem / progenitor cells and the APC gene (> 8.5 k base pairs) is too large to be cloned into the lentivirus.

This second set of experiments in cultured muscle cells showed that HMB attenuated a specific pathway involving caspase 3 and 8, PKR (RNA dependent protein kinase), and reactive oxygen species (ROS) known to activate the ubiquitin - protease pathway.

In the journal Cancer Letters, a December 2012 article uncovered that grape seed extract was effective against colorectal cancer in experiments using cultured cancer cellIn the journal Cancer Letters, a December 2012 article uncovered that grape seed extract was effective against colorectal cancer in experiments using cultured cancer cellin experiments using cultured cancer cells.

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign

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