Sentences with phrase «in cell culture media»

(A) Different biomolecules on the surface of prepared silver nanoparticles (linker not shown); (B) in cell culture media the nanoparticle - water interface is composed of ligands and ions but also of proteins, the so - called protein corona.

Not exact matches

He has more than fifteen years» experience in the life science industry, including the product development, market development and commercialization of cells, scaffolds, cell reprogramming tools and cell culture media for regenerative medicine and bioprocessing applications.

3M Delivers Early Purification with New Hybrid Purifiers, Single - use functionalised media provides scalable purification performance in cell culture

In addition to managing the cell bank, the facility makes culture media and maintains a resale storeroom for laboratory products.

One of the 12 audience members was Ben Chao, a chemical engineer with a Ph.D. — not at all qualified to make a judgment on Scott's abilities in the area of «Approaches to Developing Serum - Free Media Formulations for Mammalian Cells in Culture.»

In a typical 3D culture system, cells cling to each other, forming spheroids that float in liquid culture media or expand into a soft matriIn a typical 3D culture system, cells cling to each other, forming spheroids that float in liquid culture media or expand into a soft matriin liquid culture media or expand into a soft matrix.

Our system runs in a normal cell culture dish, and there are no pumps; we use pressure heads to perfuse media through the vasculature.

To boost their odds, the researchers bathed the cells in culture media that encourage growth of neural stem cells.

Five days post-stimulation the anti-CD3 / anti-CD28 coated magnetic beads were removed from each of the three cultures (non-transduced (NTD), AdX4 - ZFNs, and AdR5 - ZFNs) and 2.5 million cells were seeded in each of four cultures that were subsequently infected with either Bk132 (primary X4 isolate), HxB2 (lab - adapted X4 isolate), R3A (R5X4 primary isolate), or media only (mock).

Efforts to promote a selective increase in the number of endothelial cells present in primary cultures by using different media preparations (MCDB - 131, D - Valine, etc.) were largely unsuccessful.

Culture of Pig Induced Pluripotent Stem Cells without Direct Feeder Contact in Serum Free Media.

These cells produced platelet - like particles (PLPs) similar to those isolated from human peripheral blood following an additional 18 days of culture in a chemically defined media without feeder cells.

After addition of exogenous IL - 2, the levels of IFN - γ production in culture media of stimulated CD8 + T cells increased in all genotypes, again likely attributable to cell number (Fig. 3F).

Then, cells were either stimulated with methionine - choline deficient media (MCD - media; WME without choline, methionine, or glutamine and with 0.1 % BSA) for 24 hr or cultured in control medium (WME with 0.1 % BSA).

In these tests, StemFit not only delivered higher cell proliferation, but also showed characteristics such as homogeneity of gene expression compared with iPS cells cultured with four other media without any chromosomal abnormalities.

Hands - on Practical Training: Preparation of Media and Capillaries, Sperm Collection and Cryopreservation, Superovulation, Oviduct and Uterus Flushing, Handling and Culture of Preimplantation Embryos, In vitro Fertilisation, Controlled Freezing of 2 - cell embryos, Vasectomy, Plug Check of Recipients, Surgical Oviduct and Uterus Embryo Transfer

Expansion of CD34 + cells normalized relative to the values obtained in SFEM medium (dark gray bar) after culturing purified CD34 + cord blood cells for 7 days in StemSpan ™ serum -[SFEM, SFEM II (gold bar)-RSB- and animal component - free ACF (orange bar) media, and six media from other suppliers (light gray bars).

For lethal toxin experiments MNK - 3 cells were cultured in media containing 10 ng / ml IL - 7.

In addition, brains cultured with the conditioned media from GBP1 or GBP2 expressing cells revealed that diluted media induced ILP2 and ILP5 secretion more effectively.

MNK - 3 cells (an ILC3 cell line) were cultured in MNK - 3 media (DMEM (High glucose), 10 % FBS, 100 U / ml penicillin, 100 U / ml streptomycin, 2 mM Glutagro, 1 mM sodium pyruvate, 10 mM HEPES, 55 μM β - mercaptoethanol, 5 μg / ml gentamicin, 10 ng / ml IL - 7 and 10 ng / ml IL - 15).

(A, B) Culturing wild - type brains with conditioned media from GBP1 and GBP2 expressing cells induces low level of ILP2 (A) and ILP5 (B) accumulation in the insulin - producing cells.

(C, D) Culturing wild - type brains with conditioned media from GBP1 and GBP2 expressing cells decreases the densities of ILP2 (C) and ILP5 (D) signals in the insulin - producing cells.

After we confirmed there were no live cells in the media under a microscope, we used the conditioned media to culture brains from w1118 larvae as mentioned above.

Significant advancements have been made in the bioprocessing of hPS cells including the formulation of new cell culture media, the development of extracellular and acellular matrices that can replace feeder cells, the development of new enzymes for passaging and the discovery of small molecules that significantly enhance the survival and clonogenicity of dissociated cells, allowing single cell cryopreservation.

However, the industry appears to favor cells adapted to suspension cultures (e.g., HEK293), which can successfully grow in serum - free media and thus provide a heightened safety profile and reduced cost of goods (CoG)(33).

In the present study we mimicked pressure pulsations by cyclically stretching lamina cribrosa cells and collecting / purifying released exosomes from the cell culture media.

In addition to feeder free serum and LIF containing media, ES cells can be cultured in minimal serum free media (referred to as 2i) containing the MEK inhibitor PD0325901 that targets the phospho ERK branch of the FGF pathway and the GSK3 - β antagonist CHIR99021 [42In addition to feeder free serum and LIF containing media, ES cells can be cultured in minimal serum free media (referred to as 2i) containing the MEK inhibitor PD0325901 that targets the phospho ERK branch of the FGF pathway and the GSK3 - β antagonist CHIR99021 [42in minimal serum free media (referred to as 2i) containing the MEK inhibitor PD0325901 that targets the phospho ERK branch of the FGF pathway and the GSK3 - β antagonist CHIR99021 [42].

Briefly, NSCs (1 × 105 in 400 µL culture media) were seeded into the upper chamber of a 24 - well Boyden chamber with an 8 µm pore - size membrane, and tumor cell - conditioned medium (600 µL) was added to the bottom chamber.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

293FT cells (Invitrogen) were maintained in MEF media supplemented with 0.5 mg / ml Geneticin (Invitrogen) and cultured until reaching 90 — 95 % confluence before transfection.

Effect of stem cell activation, culture media of manipulated embryos, and site of embryo transfer in the production of F0 embryonic stem cell mice

One - cell embryos obtained from superovulated female mice mated with (C57BL / 6xCBA) males were cultured in KSOM media (Sigma - Aldrich) supplemented with 10 % FCS (Sigma - Aldrich) for 4 days.

Bacterial CFUs were quantified in cell - free culture media at 4 h after infection.

But in our current culture, students get information from 400 channels of television, the Internet, cell phones, and a multitude of other media outlets.

The harvested cells are sent to specialized laboratories that take the tissue, isolate the stem cells, and place them in culture media.

ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band cell — type of white blood cell Baso basophil — type of white blood cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood cells Hb, Hgb hemoglobin — iron rich protein bound to red blood cells that carries oxygen (anemia, red cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed - cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed - cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood cell — immature red blood cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed - cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet — cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood cell count (infection, inflammation, bone marrow suppression)

Helped design and carry out experimental procedures Performed Immunochemical staining Prepared Media and aliquots Conducted basic biological manipulations of bacteria Performed cell culture and seeding Participated in the Department's Quality Control, Quality Assurance, Safety, and preventive maintenance programs.