Sentences with phrase «in cell culture systems»

Flavonoids have been shown to reduce biomarkers of oxidative cell damage in cell culture systems, animal models and human studies.

Antioxidants have been shown to reduce bio-markers of oxidative cell damage in cell culture systems, as well as in many animal models and human studies.

«It is difficult to know whether the anti-amyloidogenic effect of resveratrol observed in cell culture systems can support the beneficial effect of specific diets,» Marambaud explains.

We thank Dominic Fan and Ewa Kruzel for sharing their expertise in cell culture systems, Walter Pagel for critical editorial review, and Lola López for expert assistance in the preparation of this manuscript.

«The fact that glutamine addiction has mainly been investigated in cell culture systems may have overestimated the lethality of glutamine deprivation,» said Martin Eilers, University of Würzburg, who led the study together with Kempa.

In the cell culture systems, c - MYC protein will always be at a high level.

Glutamine addiction has often been studied in cell culture systems that are genetically altered to overproduce c - MYC, a central regulator of growth and proliferation in all cells that is frequently de-regulated in cancers.

Not exact matches

Another ingredient in this skin serum is ZPRO ™, which is the ZO Protein and Antioxidant System, made of butterfly bush meristem cell culture and hydrolyzed sericin.

The trophoblasts along with blood vessel cells were added to small dextran beads that were then spun around in a container filled with cell culture fluid, creating shear stress and rotational forces to better mimic the environment at the maternal - fetal interface than static cell - culture systems.

New developments include reaction systems for catalysis research, sampling from reactors, systems for high - throughput applications in synthesis, the handling of microorganisms or cell - culturing procedures, and more.

An interdisciplinary scientist is needed to demonstrate how the mechanical properties of the culture system alter signaling in the cells.

However, this coupling does not exist in cell culture models with exogenously expressed c - MYC — the type of systems that are mainly used to investigate glutamine addiction in cancer.

«As a biologist, I needed simple yet tunable cell - culture gels that would give me a defined system to study metabolites in the laboratory.

The researchers then used a 3 - D cell culture system to coax their mutant and healthy hPSCs down the differentiation pathway into becoming kidney progenitor cells and finally the proximal tubule cells found in kidney nephrons.

In a typical 3D culture system, cells cling to each other, forming spheroids that float in liquid culture media or expand into a soft matriIn a typical 3D culture system, cells cling to each other, forming spheroids that float in liquid culture media or expand into a soft matriin liquid culture media or expand into a soft matrix.

They found that indeed, they do, and that stimulating these cells led them to kill cells infected with HIV - 1 derived from latently infected cells, both in culture and in mice engineered to have a human immune system.

protected animals»); studies on in vitro systems (whole perfused organs, tissue slices, cell and tissue cultures, and subcellular fractions); and human studies (including estimations of occupational and environmental exposure, postmarketing surveillance, epidemiology, and the ethical and strictly controlled use of human volunteers).

Our system runs in a normal cell culture dish, and there are no pumps; we use pressure heads to perfuse media through the vasculature.

We have been using cell culture models and zebrafish as an assay to determine the effects of BMP on signaling, but we also want to be able to understand what the effect of the mutation is in the tissue and organ systems.

They tested the system on cell cultures, and then in live mice with breast cancer and mice with constricted arteries in their legs.

The scientists then tried the proline / polyproline combination in a test system of adherent cell cultures that were subjected to freeze - thaw conditions.

The proposed manipulation system makes it possible to place cells in a microwell array and observe the adherence, spreading, culture, and death of the cells.

Using her new culture system, she joined forces with colleagues to research which cells in an embryo contribute to which parts of the adult animal, a process called fate - mapping.

In this tissue system, we can culture patient - derived megakaryocytes — the bone marrow cells that make platelets — and also endothelial cells, which are found in bone marrow and promote platelet production, to design patient - specific drug administration regimes.&raquIn this tissue system, we can culture patient - derived megakaryocytes — the bone marrow cells that make platelets — and also endothelial cells, which are found in bone marrow and promote platelet production, to design patient - specific drug administration regimes.&raquin bone marrow and promote platelet production, to design patient - specific drug administration regimes.»

He devised a three - chambered cell - culture system in which the chambers were separated by impermeable barriers of sterile silicon grease, preventing the diffusion of fluid from one chamber to another.

In recent years cultured Drosophila S2 cells have been used extensively as a model system to study infection and immune responses.

The immortalized endothelial cell lines established from H - 2Kb - tsA58 mice provide, for the first time, a cell culture system to examine factors regulating angiogenesis and tumor cell arrest in different organ systems.

AHERF Neurosciences Grant (G9410 -020-Y01)- Co-PI; «Differential expression and sorting of the neuron - specific class III b - tubulin isotype in human neuronal and neuroendocrine tumor cell lines maintained in an organ culture system».

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

Drawing upon the cell culture expertise of Noriyoshi Sakai, Ph.D., and Kayoko Kurita, of Fukui Prefectural University, the Japanese - U.S. team developed a system that enables immature sperm cells, or spermatagonia, taken from male zebrafish to survive long enough in vitro that they can receive foreign genes inserted by a retrovirus.

In the health care industry, many life - saving products, such as recombinant therapeutic proteins, vaccines, and gene therapy vectors, are produced from large - scale cell culture systems.

They use a wide range of tools and systems, including genetics in model organisms, cell culture, biochemistry, proteomics, and genomics.

With this information, they turned to a cell culture system to see if any Cpf1 nucleases would exhibit in vivo activity in mammalian cells.

The CRISPR / Cas system is amazingly versatile and has proven to edit the genome effectively not only in mice and flies, but also in monkeys and, yes, in human cultured cells.

It facilitates self - renewal of both pluripotent human embryonic stem cells and induced pluripotent stem cells in a chemically defined, feeder - free and animal - protein free cell culture system.

Cells grown with this culture system are maintained in an undifferentiated state with virtually no detectable background differentiation, eliminating the need for cell selection.

Bystander effects have been described in numerous in vitro cell culture systems.

This kit utilizes the DEF ‑ CS culture system, recognized for its suitability for genome engineering (Valton et al. 2014) and single - cell cloning (Feng et al. 2014), to promote reliable growth of hiPS cells in a feeder - free and defined environment.

Unlike «closed» systems, in which cells are enclosed in chips, Griffith's open platform would allow researchers to work directly with the cultured tissue — for example, by chemically inducing inflammation and testing how different drugs interact with the inflamed tissue.

A novel 3D mammalian cell perfusion - culture system in microfluidic channels.

Before and after using this system, hiPS cells are grown in the DEF ‑ CS culture system, which maintains cells as a karyotypically stable and pluripotent monolayer.

Cellartis human iPS cells from the ChiPSC22 line (Figure 2) and the ChiPSC18 line (Figures 3 — 7 and Table I) were grown in the Cellartis DEF ‑ CS 500 Culture System before editing.

Gesicle - mediated knockout of AcGFP1 hiPS cells does not alter pluripotency when performed in the DEF ‑ CS culture system.

Taken together, these data indicate that hiPS cells expanded in the DEF ‑ CS culture system can be successfully edited using gesicles while retaining their pluripotency.

The DEF - CS system is a robust culture system for efficient expansion of human induced pluripotent stem (iPS) cells in a feeder - free and defined environment.

First, cells from Cellartis Human iPS Cell Line 22 (ChiPSC22) were modified to stably express AcGFP1, then these cells were cultured using the DEF ‑ CS culture system in a 48 - well plate.

CD81 - negative hiPS cells remain pluripotent in the DEF ‑ CS culture system.

Laminin - 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno - free and biologically relevant cell culture system.

Since human embryonic stem cells grow in an adherent culture system, for cells being reprogrammed this «new culture system» is an adherent culture system (to try and mimic the conditions the embryonic stem cells want to be happy).

These results provide the validity to the newly developed hypoxia / reoxygenation culture system for examining the regulation of CSCs in breast cancer cell lines by niche factors in the tumor microenvironment and developing differential targeting strategies to eradicate breast CSCs.