Sentences with phrase «in cell suspensions»

Not exact matches

McG will get a suspension, maybe a fine, maybe a day in a holding cell, who cares?

After cells held in suspension in an agarose solution are grown around the vascular structure, a solvent can be used to wash the sugar away.

Because the acoustic tweezers operate in a vertical channel that holds the cell - containing liquid, the researchers can trap the cells in suspension or allow them to settle onto the surface of the substrate.

Coffey is part of a trial sponsored by the drug company Pfizer awaiting approval in the United Kingdom that will slip a patch of hESC - derived RPE cells under the retina instead of injecting them in a liquid suspension.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

However, in addition to depleting CD8 + and CD4 + cells, the cell suspensions were also treated with anti-Thy 1.2 monoclonal antibody plus complement to remove any putative CD4 −, 8 − T cells (i.e., double negative T cells).

The capacity of CD8 + T cells to mediate corneal allograft rejection could have been due to the CTL population that might have been present in the CD8 + T cell suspensions used in the adoptive transfer inocula.

Using an in vitro proxy assay, i.e. the ability of single cells to generate multicellular spheres in suspension (mammosphere assay), it was determined that the mesenchymal subpopulation of HMLE cells is enriched for mammosphere - forming cells.

Briefly, single - cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 - well microtiter plates (Corning Inc., Corning, NY) along with 2 × 104 51Cr - labeled BALB / c corneal epithelial or endothelial cells in a total volume of 200 μl / well.

Spleens were processed into cell suspensions through a 70 - μm strainer by a syringe plunger in RPMI 1640 with 10 % FBS and then treated with ACK lysis buffer to remove RBCs.

Microcarriers represent a viable solution by enabling anchorage - dependent stem cells to be cultured in suspension, allowing significant scale - up in bioreactors.

This process (see figure) started with spin - embryoid body (spin - EB) formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.

A cell - dissociation enzyme can be added during the harvest step in order to facilitate a single - cell suspension.

Single spleen cell suspensions from all three categories of mice were prepared in complete RPMI 1640 medium (CRPMI) containing 5 × 10 − 5 M 2mercaptoethanol (Sigma).

The cells can be grown both in suspension and as monolayer.

Treated cell suspensions were over-layered in agarose coated slides as described [19].

Single cell suspension of HCT - 116 was plated in 6 well ultra-low attachment plates in serum deprived conditions in 37 °C incubator with 5 % CO2.

Suspension culture of hepatocyte - derived reporter cells in presence of albumin to form stable three - dimensional spheroids.

The cell sheet engineering techniques have been developed in order to avoid the limitations of tissue reconstruction using biodegradable scaffolds or single - cell suspension injections.

The balloon catheter is placed and fixed in the target coronary artery and cell suspension is transfused via the catheter.

Briefly, 1 mL cell suspension (1 × 106 cells) was incubated for few minutes in rat specific Fc block solution (BD Biosciences, San Jose, CA, USA) followed by incubation with fluorescence - tagged primary antibodies - CD11b - FITC (BD Biosciences), TNF - α - PE (BD Biosciences) and NF - κB - FITC (in 1:100 dilution) at 4 °C for 30 min in dark.

Embryoid bodies (EB) are the three - dimensional aggregates formed in suspension by pluripotent stem cells (PSC), including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC).

Single cell suspensions were washed in PBS and then stained for 20 — 30 min on ice in the dark with fluorophore - labeled Abs (for a complete list see Tables 1 and 2) and fixable viability dye (eBioscience).

Cell suspensions were diluted to a final concentration of 104 cells / µl in DMEM / F12 (3 ∶ 1) and kept on ice until transplantation.

In suspension culture, they formed embryoid bodies (EBs), which contained various cell types originating from three embryonic germ layers.

Single - cell suspensions were obtained from spleen, mesenteric lymph node (MLN), and organs of mice and were stimulated in vitro with 50 ng / ml PMA and 500 ng / ml ionomycin (both from Sigma - Aldrich) for 5 — 6 h in the presence of GolgiStop or GolgiPlug (BD Biosciences).

Viral Manufacturing To manufacture viral vectors, two approaches generally are adopted for culturing purposes: scale - out of adherent - cell systems based on 2D planar technologies and scale - up of 3D suspension cell cultures in stirred - tank bioreactors.

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

However, the industry appears to favor cells adapted to suspension cultures (e.g., HEK293), which can successfully grow in serum - free media and thus provide a heightened safety profile and reduced cost of goods (CoG)(33).

However, suspension - adapted production cell lines cultured in large - scale bioreactors are more amenable to therapeutic product expression because of their enhanced scalability and elimination of detachment agents, which facilitates regulatory compliance.

PC3 - GFP cells (∼ 70 % confluent) were harvested in trypsin (0.25 %) / EDTA and resuspended in fresh medium to generate a single - cell suspension with a density of ∼ 10 cells / mL.

Sterilized cover slips were placed in each well followed by addition of cell suspensions (1 × 105 cells / mL, 2 mL per well).

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

After 8 days growing in suspension, the cell aggregates were transferred to gelatin - coated dishes containing the same medium to allow the cells to attach.

Flow cytometry allows researchers to quantify and isolate rare viable cell subsets from heterogenous populations in single - cell suspensions based on differential expression of surface antigens.

Single - cell suspension was obtained by filtering the supernatant through a 40 - μm cell strainer, and cell suspension was then gently loaded onto a layer of Histopaque - 1077 gradient (1 × 106 — 3 × 106 cells / mL HistoPaque in a total of 3 - mL volume) and then centrifuged at 400 × g for 30 min at room temperature.

Both normal and cancer breast stem cells give rise to 3D mammospheres in suspension culture.

Characterization and applications of serum - free induced adhesion in jurkat suspension cells, Biotechnology Bioengineering (2010).

Consistent with this finding, enumeration of annexin V + DCs and active Caspase - 3 + DCs in spleen cell suspensions failed to show differences in their frequency in wild - type and EBI2 - deficient mice (not shown).

Indeed, when we assessed these cells in suspension culture, overexpression of MELK significantly increased the proliferation of HMEC cells (Figure 4 — figure supplement 1A).

Indeed, we found that MELK overexpression in non-transformed HMEC - DD cells resulted in increased cell proliferation in suspension culture (Figure 4 — figue supplement 2A).

Small touches, like politely hanging up your mystical hammer on a coat rack or getting cell reception in another world, are a way of spoofing the impossible and diffusing the heavy reliance on suspension of disbelief.

For example, nearly half of all suspensions issued in California public schools during the 2011 — 12 school year were for «willful defiance,» a category of student misconduct that includes refusing to remove a hat or turn off a cell phone, or school uniform violations.

Another explanation is that CPS has no standards for in - school suspension rooms, so they are little more than holding cells that offer little or no education or counseling to help change behavior.

Instead of studying, our students are increasingly spending time in suspension or in prison cells.

The discipline policy shifts by a range of governments have yielded real change: In 2014, California became the first state in the nation to ban «willful defiance» suspensions for its youngest students — a category of misconduct that includes refusing to remove a hat, to wear the school uniform, or to turn off a cell phonIn 2014, California became the first state in the nation to ban «willful defiance» suspensions for its youngest students — a category of misconduct that includes refusing to remove a hat, to wear the school uniform, or to turn off a cell phonin the nation to ban «willful defiance» suspensions for its youngest students — a category of misconduct that includes refusing to remove a hat, to wear the school uniform, or to turn off a cell phone.

Problem: This approach would be better, but would still be biased because cell phone violations not resulting in a suspension are much less likely to be tracked.

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Show More Vehicle Equipment Exterior Features Aerodynamic multi-reflector halogen headlamps w / auto - offColor - keyed combination hard / soft shell spare tire coverColor - keyed rear spoiler w / stop lampHigh solar energy - absorbing glassLED rear lampsSide - opening rear door w / hold - open feature Color - keyed bumpersColor - keyed rear door handleFoldable pwr mirrorsIntermittent rear wiperRear privacy glassWasher - linked variable intermittent windshield wipers Show More Interior Features (10) cup / bottle holders60 / 40 split fabric - trimmed rear bench w / slide / recline function, 1 - touch fold - flat lever, height - adjustable headrest, center armrestAmber - illuminated Optitron instrument cluster - inc: speedometer, tachometer, fuel gauge, LCD odometer w / (2) tripmetersCruise control w / brake deceleration featureDirect tire pressure monitor systemDual glove boxes w / upper box one - push open / closeFender - mounted mast antennaMulti - function center console - inc: (2) illuminated cupholders, storage box, cell phone storage pocketPwr windows w / driver - side auto up / down & jam protectionRear window defogger w / timerTilt / telescoping steering wheel (3) 12V auxiliary pwr outletsAir conditioning w / air filtrationCargo area storage pockets in wheel housing w / (2) cupholdersDigital clockDoor panel storage pockets, bottle holdersDual visor vanity mirrors w / sliding extensions, roof - mounted vanity lightIlluminated entry - inc: key illumination, interior light, front map lightOutside temp gaugeRear deck storage - inc: water - repellant foldable deck boardRemote hood / fuel filler door releases Show More Mechanical Features 2.4 L DOHC SFI 16 - valve VVT - i 4 - cyl engineAutomatic limited - slip differentialElectric pwr rack & pinion steeringFront wheel driveFull - size spare tirePwr vented front / rear solid disc brakes 4 - speed ECT automatic transmissionDouble - wishbone rear suspension w / coil springsElectronic brakeforce distribution (EBD) Front / rear stabilizer barsIndependent MacPherson strut front suspension Show More Safety Features 3 - point seat belts for all seating positions w / adjustable front seatbelt anchorsChild restraint system (ISOFIX CRS) top tether anchors for all rear seating positionsDriver / front passenger advanced airbag systemDrivers emergency locking retractor (ELR) seatbeltFront crumple zoneSide impact door beams Child protector rear door locksCollapsible steering columnDriver / front passenger front seat - mounted side airbagsFront & 2nd row roll - sensing side curtain airbagsPassengers automatic locking retractor (ALR) & emergency locking retractor (ELR) seatbelts Show More Vehicle Warranty Used Cars may have a Remaining - Factory Warranty.