Sentences with phrase «in chondrocyte»

The dual role of autophagy in chondrocyte responses in the pathogenesis of articular cartilage degeneration in osteoarthritis.

Interestingly, Rho kinase inhibition under hypoxic conditions produced a more effective increase in chondrocyte - specific gene expression and synthesis of extracellular matrix components by HCS - 2 / 8 cells.

The Rho - kinase inhibition stimuli under low oxygen conditions (of 5 percent oxygen atmosphere), which is a condition corresponding to conditions in the body's cartilage, produced a more effective increase in chondrocyte - specific gene expression and synthesis of extracellular matrix components by HCS - 2 / 8 cells.

This is a developing zebrafish skeleton showing Sox9 activates a green fluorescent protein reporter in chondrocytes.

Scientists have known that both inflammation and aging lead to diminished ATP production (and so lower adenosine levels) in chondrocytes.

In the meantime, Baojin Yao, postdoctoral fellow, focused on identifying mechanisms controlling the expression of the SOX9 gene in chondrocytes (Yao et al., Nucleic Acids Res., 2015).

Thus, SOX9 expression in chondrocytes involves various transcriptional modules regulated by SOX9 itself and by other factors.

, Angelica sinensis polysaccharides stimulated UDP - sugar synthase genes through promoting gene expression of IGF - 1 and IGF1R in chondrocytes: promoting anti-osteoarthritic activity, 2014

for article Activation of α2A - adrenergic signal transduction in chondrocytes promotes degenerative remodelling of temporomandibular joint.

Not exact matches

This may entail making small holes in the bone to allow new cartilage to grow (microfracture), taking cartilage from another part of the athlete's knee and transplanting it into the defect (osteochondral autograft transfer), taking cartilage cells from the knee and then having them grown in a lab for later re-implantation (autologous chondrocyte implantation), or taking cartilage from a person who has passed away and placing it in the defect (osteochondral allograft transfer).

For instance, to make a rod with collagen fibers aligned along its length (like a tendon) they cultured chondrocyte cells in a dog bone - shaped mold with loops on either end.

Then, to boost the number of cells, which is another hurdle in tissue engineering, the researchers mixed the chondrocytes with human mesenchymal stem cells from bone marrow.

Preliminary data from in vivo testing over 60 days show the combination does indeed encourage chondrocyte and cartilage production.

One exciting potential for Permacol is that it can be seeded with the patient's own cells, so that in the future it may be possible to repair cartilage or bone by seeding with chondrocytes or osteocytes.

The results showed that, when applied, a long - term inhibition of Rho - kinase signaling increased the expressions of chondrocyte - specific genes and differentiation markers in human chondrosarcoma 2/8 cells.

«This kind of markers can be useful to monitor responses during chondrocyte in vitro culturing, but also be applicable to monitor status of the chondrocytes during the different kinds of cartilage therapies.

«Our paper is a blueprint of how the cartilage - producing cell, called a chondrocyte, is made and maintained by Sox9,» said He, a postdoctoral research associate in the lab of Andy McMahon, director of the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC.

The findings suggest that reductions in the number of cartilage - producing cells, and greater risk for osteoarthritis, may be driven not just by lower adenosine levels but also by lower levels of the protein on the surface of chondrocytes designed to receive and pass on adenosine's signal.

Furthermore, organ cultured cartilage from ACH mice treated with statin resulted in increased expression of the aforementioned three genes and also in Runx2 and Col10a1, which indicated that statin stimulated both the differentiation and maturation of ACH mouse chondrocytes.

Additional tests in tissue samples from osteoarthritic patients who had joint replacements at NYU Langone found similarly increased levels of adenosine A2A receptors on chondrocytes.

Cronstein and his team also found that levels of adenosine A2A receptors went up on rat chondrocytes when osteoarthritis was present, in what the researchers say was a «failed attempt» to compensate for the loss of adenosine from the energy - processing (metabolic) changes underlying the inflammation.

Special focus is placed on Rho - kinase inhibition, relating to its potential to promote and support extracellular matrix production in cultured chondrocytes and its role in fibroblast cells as a part of direct chemical cellular differentiation.

A short - term inhibition of Rho - kinase failed to induce extracellular matrix production in fibroblasts or in HCS - 2 / 8 cells, while its long - term exposure increased the expressions of chondrocyte - specific genes and differentiation markers, and also promoted the synthesis of sulfated glycosaminoglycans by chondrocytic cells.

Thus, Rho - kinase inhibition at low oxygen tension can be regarded as a potential way to enhance extracellular matrix production and maintain a chondrocyte phenotype in cell - based tissue engineering applications.

Downregulation of microRNA - 448 inhibits IL - 1ß - induced cartilage degradation in human chondrocytes via upregulation of matrilin - 3.

Perivascular cells, including pericytes in the smallest blood vessels (e.g., microvessels) and ARCs around larger ones, express mesenchymal stem cell markers and bear a multi-differentiation fate potential (differentiate into osteoblasts, chondrocytes, adipocytes, smooth muscle cells and myocytes) similar to that documented for MSCs in vitro.

Stage - specific embryonic antigen - 4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.

Using chondroitin sulfate to improve the viability and biosynthesis of chondrocytes encapsulated in interpenetrating network (IPN) hydrogels of agarose and poly (ethylene glycol) diacrylate.

In addition to iPS cells derived from progeria - patients, the researchers successfully applied their method to adult mesenchymal stem cells, which can differentiate into a variety of cell types, including adipocytes, osteoblasts, chondrocytes, cardiomyocytes, and, as described lately, beta - pancreatic islets cells.

Kenji demonstrated that SOXC proteins fulfill this function primarily through actions in perichondrium cells, but also through actions in growth plate chondrocytes.

In an attempt to bring patient - specific induced pluripotent stem cell (iPSCs) technology closer to the clinic, researchers have created iPSCs from patient - derived chondrocytes, using an non-integrative reprogramming method, and used these to then create large numbers of cartilage producing cells.

New SOX chondrogenic trio projects in the laboratory are addressing the roles of the proteins in chondrogenic precursor cells and in adult articular chondrocytes.

Elisseeff J, McIntosh W, Anseth K, Riley S, Ragan P, Langer R. «Photoencapsulation of chondrocytes in poly (ethylene oxide)- based semi - interpenetrating networks.»

Based on previous protocols [5, 6] they have now created a 3D protocol for chondrogenic lineage differentiation via the generation of a putative chondrogenic progenitor cell population, and have found that using this protocol C - iPSCs can be readily differentiated into cartilage in a manner comparable to that of mature chondrocytes [7].

The choice of the somatic cell for reprogramming, the reprogramming technology chosen, and the differentiation techniques utilised, all work synergistically towards the production of mature iPSCs - derived chondrocytes which are comparable to patient - derived chondrocytes, in line with Good Manufacturing Practice guidelines for an «off - the - shelf» stem cell product.

However, autologous chondrocytes lack long term proliferation and functionality in vitro, and so another cell source is being searched for which could produce chondrocytes with an increase in functional lifetime.

We investigated genes and pathways that mark OA progression in isolated primary chondrocytes taken from paired intact versus degraded articular cartilage samples across 38 patients undergoing joint replacement surgery (discovery cohort: 12 knee OA, replication cohorts: 17 knee OA, 9 hip OA patients).

Both control iPSCs derived from BJ fibroblasts (f - iPSCs) and chondrocyte derived - iPSCs (C - iPSCs) were fully pluripotent and genomically stable (See figure), and were first differentiated in a monolayer exposing the iPSCs in a defined, three stage manner to activin A, WNT3A, FGF2, BMP4, follistatin, GDF5, and neurotrophin 4.

Chlorogenic acid suppresses interleukin - 1β - induced inflammatory mediators in human chondrocytes.

Inside the joint, the cartilage cells (chondrocytes) rest like bricks in a glistening mortar made of resilient material.

In addition to the promotion and maintenance of joint integrity, chondrocytes also secrete a special substance that is added to the synovial fluid in the joint capsulIn addition to the promotion and maintenance of joint integrity, chondrocytes also secrete a special substance that is added to the synovial fluid in the joint capsulin the joint capsule.

These substances are well known for promoting the increased production of chondrocytes or the cells that make up the cartilage found in the ends of adjoining bones.

These are composed of specialized cells that are called chondrocytes which function in the maintenance of the integrity of the cartilage.

The results showed that the combination of avocado / soybean unsaponifiables (ASU), glucosamine hydrochloride, and chondroitin sulfate is effective in equine cartilage cells (chondrocytes) and bone cells (osteoblasts) at inhibiting expression and decreasing production of mediators involved in joint cartilage breakdown.

Excessive biomechanical loading of the inadequately supported epiphyseal articular cartilage leads to secondary disturbances in the nutrition, metabolism, function and viability of the chondrocytes in the developing joint surface.