Sentences with phrase «in confocal»

Guts were fixed, stained to reveal GFP, Prospero and Delta, and examined in a confocal microscope.

In confocal microscopes, optical sectioning of a specimen is based on discriminating the out - of - focus reflected light by using a pinhole.

Experience in Confocal and Super-resolution microscopy as well as image analysis is highly desireable.

Aberrations in confocal and multi-photon fluorescence microscopy induced by refractive index mismatch.

So the Bessel beam can scan a cell with the same total flux as the light spots used in confocal microscopy, but that flux is distributed among the seven beams and the cell isn't flooded with excess light; thus, the cell isn't damaged nearly as quickly.

In confocal microscopy the focal plane is imaged with a single, intense point of light, centered between two cones of light that flood the rest of the sample.

Kozlowski tackled that problem computationally; using the popular MATLAB programming environment, she and her colleagues developed an algorithm that classifies the shapes of microglia in confocal microscopy images with 90 % accuracy.

GET YOUR GLOW ON The reef coral Montipora capitata naturally fluoresces in red and cyan, as seen in this confocal microscopy image.

Not exact matches

Helped by the recent development of fiber - optic - bundle - coupled laser - scanning confocal fluorescence imaging (Confocal Laser Endomicroscopy — CLE), which allowed the scientists to image blood flow more deeply in the brain than everconfocal fluorescence imaging (Confocal Laser Endomicroscopy — CLE), which allowed the scientists to image blood flow more deeply in the brain than everConfocal Laser Endomicroscopy — CLE), which allowed the scientists to image blood flow more deeply in the brain than ever before.

He studies DNA and RNA quadruplexes, nucleic acid structures that were first visualized via confocal microscopy in fixed, dead cells.

«Moving to non-invasive and repeatable methods of nerve fiber measurements such as in vivo corneal confocal microscopy would enhance study of peripheral neuropathy by enabling early detection of damage, progression of nerve fiber deterioration, and enable assessment of therapeutic strategies in the SIV / macaque model,» explains Dr. Mankowski.

«Furthermore, adapting in vivo corneal confocal microscopy for use in tracking HIV - induced PNS damage in patients may be of great value to identify early PNS damage independent of performing skin biopsies.»

Unlike nerves elsewhere in the body, the structure of the living corneal nerve can be seen and measured using a technique called confocal microscopy.

Together, these findings demonstrate that emerging noninvasive techniques to measure corneal nerve fiber alterations such as in vivo corneal confocal microscopy may be useful clinical tools to screen for and monitor progression of peripheral neuropathy in HIV - infected patients.

Using chemicals like these in combination with new imaging tools, such as the multi-photon confocal microscope, has enabled researchers to explore the minuscule world of the neuron and observe brain cells in action with far more precision.

The plankton molts were floating in seawater in a dish and imaged by laser scanning confocal microscopy.

Dr. Preul directs the Neurosurgery Research Laboratory of the Barrow Neurosurgical Institute, which has carried out the pioneer work in applying and developing confocal laser endomicroscopy for brain tumors.

In his early career he was also an influential inventor, creating the first confocal scanning microscope, a version of which is now standard in labs worldwidIn his early career he was also an influential inventor, creating the first confocal scanning microscope, a version of which is now standard in labs worldwidin labs worldwide.

In their new study, they adapted DNA - PAINT technology to microscopes that are widespread among cell biology laboratories, called confocal microscopes, and that are used by researchers to image whole cells and thicker tissues at lower resolution.

In addition to fruit flies, they successfully used the program to analyze images of zebrafish and mice, as well as data collected from a commercial light sheet microscope and a commercial confocal microscope.

The researchers use standard confocal microscopy to zero in on the constant, jiggling motions of a cell's particles.

The researchers use standard confocal microscopy to zero in on the constant, jiggling motions of a cell's particles — telltale movements that can be used to decipher a cell's stiffness.

The figure shows a confocal microscopy image of a mammalian cell undergoing apoptosis with the mitochondria stained in magenta and the protein Bax in green.

Grape - like structures of viral replication territories in the nucleus of a human cell visualized by click - chemistry and immuno - fluorescence confocal microscopy.

The ultimate dream is to take STEM into three dimensions with confocal electron microscopy, which images a material in slices by changing the focus of the beam.

The automated confocal micro-extensometer (ACME)-- developed by a team of researchers in Europe and the US — allows scientists to measure spatial variation in the mechanical properties of plant cells with unprecedented accuracy.

Using confocal laser microscopy and electron microscopy, coupled with the use of advanced synchrotron techniques at the Swiss Light Source, in Villigen (Switzerland), and at the Canadian Light Source in Saskatoon, Canada, the team was able to show that not only the morphology of microbial twisted structures is preserved after long incubations under diagenetic conditions, but also organic remnants can be detected in the mineralized twisted structures.

In a recent study published in the journal NPJ Biofilms and Microbiomes, they precisely measured the surfaces of biofilms using confocal reflection light microscopIn a recent study published in the journal NPJ Biofilms and Microbiomes, they precisely measured the surfaces of biofilms using confocal reflection light microscopin the journal NPJ Biofilms and Microbiomes, they precisely measured the surfaces of biofilms using confocal reflection light microscopy.

Although traditional in vivo imaging tools, such as widefield and confocal microscopy, and newer ones, such as light - sheet microscopy, can image in three dimensions, they sacrifice substantial spatiotemporal resolution to do so and, even then, can often be used for only very limited durations before altering the physiological state of the specimen.

To verify these results, researchers in Ozcan's lab used other imaging devices, including a scanning electron microscope and a photon - counting confocal microscope.

The authors came to their conclusion after studying skin cells in mouse models using a confocal and two - photon microscopes, and developing mathematical models.

Confocal microscope images show far fewer horizontal cells generated in mice without Onecut1 (bottom panels) compared to those in normal mice (top panels).

Additionally, high magnification confocal analysis revealed that the remaining hemocytes in these animals appeared morphologically identical to those overexpressing Bax (Figure 3F).

Our success in these areas is complemented by our substantial investment in cutting - edge facilities for nuclear magnetic resonance, protein crystallization, X-ray crystallography, transgenics, histochemistry, electron microscopy, confocal microscopy, and fluorescence activated cell sorting.

Embryonic hemocytes lend themselves beautifully to live imaging studies since fluorescent probes can be expressed specifically in these cells using hemocyte specific promoters and their movements subsequently imaged within living embryos using confocal timelapse microscopy.

Confocal analysis revealed that apoptotic hemocytes are very different in morphology to those exposed to Mcf1 appearing hugely swollen and containing fluorescent puncta having engulfed their dying neighbouring hemocytes (Figure 3E).

State - of - the - art light - sheet and confocal microscopes allow recording of entire embryos in 3D and over time (3D + t) for many hours.

This is a dynamic process in which the bacteria are recognised by, and adhere to, the phagocytes in a process that can be dramatically seen in real time using time - lapse confocal microscopy.

Korenberg's team is developing a 3D coordinate system to align various types of neuroimaging data in the macaque brain, from whole - brain MRI connectivity to single - cell confocal data and, for some areas, subcellular resolution with electron microscopy.

In the first type, which was conducted at Rice, the team used confocal microscopy to film «giant unilamellar vesicles» (GUVs), synthetic membrane - enclosed structures that are about the same size as a living cell.

Schopf used several techniques to analyze the fossils, including Raman spectroscopy — which enables scientists to look inside rocks to determine their composition and chemistry — and confocal laser scanning microscopy — which renders fossils in 3 - D.

IDMIT is an infrastructure for preclinical research in infectious diseases and immunology which is certified ISO9001 and which includes 1) A large animal facility with capacity to host NHP in BSL2 and BSL3 containment, 2) State - of - the - art laboratories for cell biology, immunology, molecular biology, flow cytometry and mass cytometry (CyTof), cell - sorting and confocal microscopy in BSL3 containment; 3) A biological resources centre with high storage capacity; 4) Highly innovative technologies for in vivo imaging of large animals in BSL2 and BSL3 containment, including a two - photon microscope, a PET - CT facility, and several optic based technologies (fibered endo - microscopy, near infra - red imaging).

Druckenmiller houses a specialized laser scanning confocal microscope (the only one of its kind in the state of Maine), as well as a scanning electron microscope and a number of conventional fluorescent microscopes, all of research quality.

Neurobiologist Craig Bailey uses confocal microscopy to track the learning - related changes in synapses of the sea slug Aplysia.

We will consider near - infrared, confocal endomicroscopy (two - colour CellVizio technology), two - photon microscopy, single photon emission tomography (SPECT) and positron emission tomography (PET) scan platforms available from CEA, HZI and INRA, and will develop new tracers to tag antigens, adjuvants, particles / vehicles and vectors for the in vivo visualisation of dissemination and biodistribution.

Confocal microscopy of convertases and known disease - related human convertase mutants in cells and tissues will also be used to shed light on subcellular targeting mechanisms.

Computerized image analysis of cell - cell interactions in human renal tissue by using multi-channel immunoflourescent confocal microscopy Proc.

A microscope and its parts, image formation, Köhler illumination, optical aberrations, types of lenses, phase contrast, interference contrast, polarization, fluorescence microscopy, laser confocal microscopy, two - photon confocal microscopy, superresolution microscopy, study of dynamic processes in living cells, immunofluorescence.

We, therefore, treated macrophages of six AD patients and 3 controls by curcuminoids in vitro and measured Aβ uptake using fluorescence and confocal microscopy.

In recognition of their work in developing the scanning confocal microscope to the point where it is used in hundreds of research laboratories worldwide and reveals microstructures not discernible by other methodIn recognition of their work in developing the scanning confocal microscope to the point where it is used in hundreds of research laboratories worldwide and reveals microstructures not discernible by other methodin developing the scanning confocal microscope to the point where it is used in hundreds of research laboratories worldwide and reveals microstructures not discernible by other methodin hundreds of research laboratories worldwide and reveals microstructures not discernible by other methods.