The researchers removed the organ of Corti, which is housed within a seashell - shaped cavity called the cochlea and contains sensory hair cells, from newborn mice and kept the cells alive
in culture plates.
After collecting biopsies from banked human donor eyes, the team expanded the numbers of cells
in a culture plate using human serum to nourish them.
The research highlights the importance of studying the effects of drugs not only on the tumor cells
in a culture plate, but also on the interaction of the tumor cells with their own microenvironment.
Not exact matches
In Roof's words, the tectonic
plates underlying American
culture are shifting.
Martinez says the new license
plates will send a strong message about the significant role that chile plays
in the state's
culture.
Tatura is home to the Tatura Racecourse Reserve, where the Tatura & Shepparton Racing Club Inc hosts a minimum of three full TAB race meetings each season, including the iconic Italian
Plate Festival
in December - a celebration of the local Italian community and
culture - and the Tatura Easter Cup.
Written by our friend Rachael of La Fuji Mama The Hawaiian
plate lunch is an institution
in Hawaiian food
culture — an inexpensive
plate of comfort food that can be purchased at restaurants and roadside stands all over the state.
This is the
plate in our
culture,» he said.
This is a
culture plate of Lactococcus, milk fermentation tubes and mini-cheeses made
in Dr Mark Turner's lab.
«
In the last few years, it has become possible to order
cultures of human cells for testing, but they're grown on a
plate, a two - dimensional environment,» says Radisic.
Examining agar
plates cultured from anthrax
in the letters, investigators spotted mutant colonies and then sequenced them.
If the cells grew on
culture plates, the team inferred that the human gene could fill
in for its yeast equivalent.
COS - 7 cells,
plated at 70 % confluence
in 100 - mm tissue
culture dishes, were transfected with vector alone or pcDNA3 - ACVR1 (c. 617G) or pcDNA3 - ACVR1 (c. 617A).
B, CSCs were isolated from 231BrM infected with KLF4 or control lentivirus and
cultured in low - attachment
plates.
D, the same primary breast cancer cells infected with KLF4 or control lentivirus were
cultured in low - attachment
plates and the number of mammospheres was counted after 10 days.
The tumor cells were dissociated and the cells were infected with KLF4 lentivirus and
cultured in low - attachment
plates.
C, primary breast cancer cells isolated from patients with advanced cancer were directly infected with KLF4 or control lentivirus, and the CSC population (CD24 − CD44 + ESA +) was measured by FACS after
culturing the cells for 72 hours
in low - attachment
plates.
For intracellular cytokine staining, lymphocytes were
cultured in 96 - well flat - bottom
plates at 1 × 106 cells / well
in 200 μl RPMI 1640 supplemented with 10 % FBS
in the presence or absence of GP33, GP276, and NP369 peptides.
This process (see figure) started with spin - embryoid body (spin - EB) formation from
cultures of single - cell hiPSC suspensions
in 96 well
plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.
Thirdly, the current, almost universal dependency of organoid
culture on the use of Matrigel as a replacement for the function of the extracellular basement matrix
in providing structural support and survival signals to the epithelial cells hampers clinical application, considering its origin from a mouse sarcoma cell line, its poorly defined composition and its mechanical rigidity after
plating.
First, cells from Cellartis Human iPS Cell Line 22 (ChiPSC22) were modified to stably express AcGFP1, then these cells were
cultured using the DEF ‑ CS
culture system
in a 48 - well
plate.
Delaney et al. [4] who have shown that cord blood - derived CD34 + cells
cultured in StemSpan ™ medium with cytokines, on
plates coated with Notch ligand, are expanded ex vivo while still maintaining their ability to engraft into immunocompromised mice.
Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic
culture for 5 days results
in radial migration of EGFP + pyramidal neurons to the cortical
plate (CP) and the projection of their axon
in the intermediate zone (IZ).
Assays for virus - specific neutralizing antibodies were performed on serial 2-fold diluted samples of each serum using 2 % FBS - DMEM as the diluent
in 96 - well tissue
culture plates (Falcon 3072); the final volume of the serially diluted samples
in each well was 60 µl after addition of 120 TCID50 of SARS - CoV
in 60 µl into each well.
For cell proliferation assay, 5 × 104 cells were seeded
in triplicate wells per cell line per time point
in 12 - well tissue
culture plates.
To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and
plated on polyornithine - coated tissue
culture dishes
in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).
Unfortunately, inclusion of the 2i
in our
culture system resulted
in massive death of irradiated MEF cells
plated at a relatively low density, suggesting that the 2i strategy might not be compatible with irradiated MEF cells.
For intracardiac injections, cells were harvested from subconfluent
culture plates, washed with phosphate - buffered saline (PBS), and resuspended at 106 / ml (1833) or 5 × 106 / ml (SCP28)
in PBS; 0.1 ml of the suspended cells was injected into the left cardiac ventricle using 30 - gauge needles.
To induce cardiomyocyte differentiation, compact and well - shaped EBs formed
in suspension
culture containing 10 % of FBS for 6 days were
plated in 24 - well
plates in the presence of 15 % of FBS.
Briefly, NHLF cells were grown
in 96 - well CoStar tissue
culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours
in 100 µL complete medium per well.
In this instance we did not detect a plating difference in the populations and approximately 16 % of the deposited cells survived to give rise to day 10 cultures (unpublished data
In this instance we did not detect a
plating difference
in the populations and approximately 16 % of the deposited cells survived to give rise to day 10 cultures (unpublished data
in the populations and approximately 16 % of the deposited cells survived to give rise to day 10
cultures (unpublished data).
«Before, you could
in principle test chemical compounds of interest against hundreds of cell lines, but it would require a lab member going to a freezer, taking out hundreds of vials — one for each cell line — and setting up hundreds of tissue
culture plates.
Cells were
plated in 12 - well
plates at 0.8 × 105 cells / well (A427) or 1.8 × 105 cells / well (H2122)
in RPMI medium containing 10 % FBS and
cultured overnight.
NHLF cells were
cultured in 96 - well Optilux
plates as described
in Materials and Methods.
After 3 days of RA treatment, the EBs were
plated on the matrigel - coated
culture dishes
in the N2B27 medium for several days.
EBs were
cultured in 6 - well ultra-low attachment
plates (Corning, Lowell, MA), and the
culture medium was changed every 3 days.
Inverted microscopes are used to image lives cells
in culture (flasks, dishes, or multi-well
plates) using transmitted light, phase contrast, DIC, or fluorescence modes.
Cells were
plated in six - well
plates at 2.5 × 105 (A427) or 4 × 105 (H2122) cells / well
in RPMI 1640 medium containing 10 % FBS and
cultured for 24 h.
Culture medium was collected, and PGE2 levels were determined using a PGE2 EIA kit (Cayman Chemicals).
(A) BaF3 cells control or stably transduced with wild - type or with V617F, Y931C or V617F / Y931C mutant were washed 3 times
in PBS to remove the residual IL - 3 and seeded
in 6 - well
plates at a concentration of 100,000 cells / mL
in culture medium.
These inserts are added to wells containing complete
culture medium with 10 % FBS as chemoattractant
in 24 - well fluorescence opaque
plates allowing only monitoring of fluorescence from the bottom.
E, following 48 hours
in 21 % or 1 % oxygen ± Doxycycline (Dox)- inducible shRNA lines to Notch1 (MCF7Notch1) and Notch4 (MCF7Notch4) were
plated in mammosphere
culture.
Cells were then
plated in 24 - well tissue
culture treated
plates and incubated
in a humidified 5 % CO2 incubator at 37 °C.
The skin biopsies were washed
in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell
culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and
cultured at 37 °C
in a 5 % CO2 incubator.
Cells were
cultured in 21 % or 1 % oxygen for 48 hours following transfection and then
plated in mammosphere
culture.
Bulk -
cultured PC3 or PC3 - GFP cells, or clone - derived
cultures were
plated on glass coverslips and used
in immunofluorescence staining for CD44, α2β1 integrin, and β - catenin using monoclonal antibodies and protocols described previously (27 — 29).
Sorted cells
cultured in 48 - well
plates for 17 days were washed once with PBS and fixed for 5 min.
A, following 48 hours
in 21 % or 1 % oxygen, ± YC1 cells were
plated in mammosphere
culture.
To eliminate the possibility of any undifferentiated cells
in the
cultures accounting for this observation, DMSO - differentiated cells were treated with the anti-proliferative agent cytosine arabinosidase (Ara - C), starting two days after aggregate
plating.
B, following 48 hours
in 21 % or 1 % oxygen, cells were
plated in mammosphere
culture.
C, N1 - ICD was expressed
in 231 cells
in 21 % or 1 % oxygen and cells were
plated in mammosphere
culture.