Because ATRA could cause IFNα synthesis and secretion in NB4 cells by up - regulating IRF - 1 (23), we thus quantitated the IFNα
in culture supernatant of ATRA - treated NB4 cells.
Our lab uses this kit routinely to to quantify p24 levels
in culture supernatant.
Not exact matches
Hybridoma
supernatants were screened for anti-rGRF activity by use of a pituitary
culture assay system that can detect growth hormone - releasing factor
in the femtomole range.
Both systems can simultaneously detect many targets such as cytokines, chemokines and inflammatory biomarkers
in a single sample that can be serum, plasma and tissue
culture supernatants.
UNISI provides expertise
in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers
in multiple samples (including serum, plasma and tissue
culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.
The group has over 10 years» experience with this methodology, with specific expertise
in cytokine / chemokine detection
in tissue
culture supernatants and plasma samples with panels containing up to 42 analytes.
Both technologies allow the simultaneous detection of multiple factors
in serum, plasma and tissue
culture supernatants.
Vaccinia virus (Western Reserve) recombinant for the codon - optimised gp120 gene from HIV - 1 (Bx08) expressing gp120 protein
in (VERO) cell
culture supernatant.
In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asi
In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV
in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asi
in serum and cell
culture supernatants using a Taqman probe containing locked nucleotides,
in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asi
in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating
in Africa and Asi
in Africa and Asia.
Cell debris was removed from
culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m
in a BECKMAN 70Ti rotor for 3 hrs.
Assessment of hASC - CM composition found high expression of various human growth factors (IL ‐ 6, 8, 12, eotaxin, IP10, MCP ‐ 1, VEGF, and TIMP ‐ 1)
in the
supernatant following the co-
culture of hASCs with islet cells, while IP10, eotaxin, VEGF, and TIMP ‐ 1 became increased with time during islet co ‐
culture, suggesting the presence of paracrine cross ‐ talk between islets and hASCs.
To characterize the isolate that replicated
in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue
culture supernatants.
After 24 hours (on day 1) the mixed viral
supernatant was removed, the cells were washed twice with PBS and then
cultured in fresh MEF medium.
UNISI will provide expertise
in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers
in multiple samples (including serum, plasma and tissue
culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.
On day 3 the mixed viral
supernatant was again removed, the cells were washed twice with PBS and then
cultured in fresh MEF medium.
In addition, we showed that, although the
supernatants from each of the HCMV - infected
cultures contained infectious virus, the viral copy number and the quantity and timing of virus production varied among the various organ - specific MSC.
These changes were accompanied by severe EC damage, decreased E-cadherin RNA level, elevated IFN - gamma
in splenocyte
culture supernatant, and production of significant IgM antibody against intestinal microbiota.
The
supernatant, the medium
in which the
culture is grown, contains a multitude of beneficial byproducts of the growth process, including vitamins, enzymes, antioxidants, and immune stimulators.