Sentences with phrase «in cultured cells»

Ellagic acid is a hunter of cancerigenous chemicals, inactivating them, impeding the capacity of other chemicals to trigger abnormal mutations into pathogens Moreover, Ellagic Acid from meeker red raspberries inhibits binding of carcer causing material to DNA, reducing the extent of the condition in cultured cells vulnerable to carcinogens.

In cultured cells, knocking down miR - 142 significantly reduced the organoid formation capacity of BCSCs.

The two components of Cas9 / gRNA have shown high DNA cleavage activity in cultured cells [6], [7], C. elegans [10], zebrafish [11] mice [12] and pigs [13].

In the current study, published online in eLife, Rohban, Carpenter, and their colleagues overexpressed 220 genes one - by - one in cultured cells, dyed the cells, and searched for signatures associated with each gene.

During this round compounds are tested in cultured cells or animals to find out how effective they are and whether they have any toxic effects.

Cenix BioScience is a contract research organization focused on the discovery and pre-clinical development of novel therapeutics, specializing in advanced applications of RNA interference (RNAi) gene silencing combined with high content phenotyping in cultured cells in vitro and in animals in vivo.

Here, we examine wild - type huntingtin's localization in cultured cells by expressing the full - length human protein tagged with enhanced green fluorescent protein (EGFP) within its unspliced genomic context.

Enriched by the rapid evolution of these technologies, TEFOR - TACGene has developed a solid expertise in the design, production and use of TALE - N and CRISPR / Cas9 systems, both for its own research projects in cultured cells and in collaboration, in many model organisms, including the rat, zebrafish, Drosophila, and Xenopus as part of the National Infrastructure in Biology and Health TEFOR supported by Investissement d'Avenir programme (2012 - 2019).

Develop KI techniques using modified oligonucleotides in cultured cells and in model organisms

In this course, trainees will perform experiments involving both classical physiological models, as well as modern reductionist approaches and confocal microscopy to follow trafficking of transporter proteins in cultured cells.

He presented his finding that mitochondrial dysfunction can induce senescence in cultured cells at the SENS6 Conference at the University of Cambridge.

Dr. Huizing demonstrated that elimination of the single mutant allele using a synthetic small interfering RNA (siRNA) rescued the abnormal phenotype in cultured cells from sialuria patients.

Programmable nucleases, ZFN, TALEN and RGENs enable gene knockout in cultured cells and organisms by producing site - specific DNA double - strand breaks, whose repair via error - prone non-homologous end joining (NHEJ) or microhomology - mediated end joining (MMEJ) gives rise to frameshift mutations.

The Cravatt laboratory soon developed powerful inhibitors of the newly discovered enzymes, and the two labs began working together, using the inhibitors and genetic techniques to explore the enzymes» functions in vitro and in cultured cells.

The team found that DHEA speeds up the circadian rhythm in human cells, as well as in cultured cells and tissues of mice.

Instead of testing the drugs in cultured cells as he might have done in the past, Califano tests them in PDX mice, developed individually for each patient in the trial.

The Krainer team, in collaboration with a team at Isis Pharmaceuticals, surveyed a large number of antisense oligonucleotides (ASOs) and found that some of these ASOs are able to correct the mRNA splicing defect in cultured cells from SMA patients.

Several tests in cultured cells indicated that ganciclovir would cause the death of HSV - TK - expressing stem cells, and testing in the mouse model confirmed that administration of the drug after successful treatment of brain metastases successfully eliminated therapeutic stem cells that also expressed HSV - TK.

After testing the suppressive efficacy of the PshRNA agent in cultured cells, they injected it into the mouse's eyes.

Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele in cultured cells.

With the help of a library of dengue antibodies extracted from people with the disease, Screaton's team discovered that the dengue antibodies enhanced Zika infection in cultured cells.

When the researchers turned off SIRT1 genes in cultured cells, the cells secreted less than half as much glucose as unaltered cells.

Previous research showed that COX - 2 inhibitors cut viral production in half in cultured cells, but how they did so was unclear.

The researchers found that overproducing the microRNAs in cultured cells cut down the concentration of E2F1, a protein involved in a cancer growth pathway, suggesting that, in healthy cells, the microRNAs keep cell growth in check.

In cultured cells, drugs that inhibit Cdk1 prevented HIF - 1alpha levels from falling and restored its ability to halt cell division, suggesting they may be effective treatments for certain cancers.

Bassem Hassan (VIB / KU Leuven): «Since we show that APP and APPL show similar activities in cultured cells, we suspect that APP in the human brain functions in the same manner as APPL in the brain of fruit flies.

The team first tested the antibody in cultured cells engineered to express the Nav1.7 sodium channel.

Now that they have shown how this defense mechanism works in cultured cells, they hope to study the importance of LINE - 1 jumping and the defenses against it in specific diseases.

This soluble factor was found at higher levels in the blood of animals with lung tumors, could increase the activation of osteoblasts and contributed to the maturation of neutrophils in cultured cells.

When the Stanford team measured pyrophosphate levels in cultured cells derived from ank and normal mice, they found that the chemical accumulated in cells from the ank mice but decreased in the culture medium.

Further analyses of NMP in cultured cells and live mice confirmed the solvent's antimyeloma activity.

By studying the same process in cultured cells, the scientists found that the light stimulated a molecular chain reaction that activated a normally dormant protein called TGF - beta 1.

Upon expression in cultured cells, the new subunits yielded prominent, typical glutamate - and NMDA - activated currents only when they were in heteromeric configurations with NR1.

When tested in cultured cells, the compound specifically activated chaperone - mediated autophagy without touching other pathways.

The technology substantially expands our ability to change gene expression in cultured cells and animals..»

Researchers from Duke University had previously used CRISPR to correct genetic mutations in cultured cells from Duchenne patients, and other labs had corrected genes in single - cell embryos in a laboratory environment.

In November 2010 Japanese researchers announced online in Analytical Chemistry that they had built a chip that simultaneously tests how liver, intestine and breast cancer cells respond to cancer drugs, and in February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cells.

The scientists also generated a panel of (reconstructed) ancestral and existing TRIM5 genes (19 total), expressed them in cultured cell lines, and exposed the cells to 16 different retroviruses (lentiviruses and others) to see which TRIM5 versions conferred resistance to which viruses.

An analysis of all gene transcription in the cultured cell lines turned up a large gene complex in which gene expression differed conspicuously in cells from patients compared to controls.

Not exact matches

Applied to cell cultures in a petri dish, it works.

The trial was actually supposed to launch back in August but was delayed because growing and culturing the genomically edited cells took longer than originally expected.

Avita Medical, which formed after the merger of Clinical Cell Culture and Visiomed Group, has new contracts under way in Greece and Mexico.

Based on a 3D image such as an MRI scan, Aspect's machine builds relatively complex organic structures out of a «hydrogel» embedded within cells taken from the body and grown in a cell culture.

In September 2015, the two men moved to the Bay Area and started culturing cow muscle and connective tissue cells.

Animal cells are cultured from stem cells and incubated in a «bioreactor» into tissue that can be «harvested» and formed into familiar foods like meatballs, patties, and fish sticks.

Post, of MosaMeat, who created the first hamburger from cultured animal cells in 2013 with backing from Google billionaire Brin, believes some of his competitors have set unrealistic timelines to market in part because that's what tech investors want to hear.

The company debuted its first synthetic meatball in 2016 and followed up with the world's first cell - cultured chicken and duck earlier this year.

Rather than obtaining meat from animals raised on environmentally destructive factory farms and slaughtered in filthy slaughterhouses, clean meat is produced by taking a small sample of animal cells and replicating them in a culture outside of the animal.

Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs of the growth medium necessary for culturing the desired cells.

In my cell biology course, we investigate the biology and chemistry of a cell surface receptor that helps induce good feelings in us when it binds to a chemical compound found in incense; this may help explain why so many different cultures and religions have independently evolved the use of incense in their ceremonies and ritualIn my cell biology course, we investigate the biology and chemistry of a cell surface receptor that helps induce good feelings in us when it binds to a chemical compound found in incense; this may help explain why so many different cultures and religions have independently evolved the use of incense in their ceremonies and ritualin us when it binds to a chemical compound found in incense; this may help explain why so many different cultures and religions have independently evolved the use of incense in their ceremonies and ritualin incense; this may help explain why so many different cultures and religions have independently evolved the use of incense in their ceremonies and ritualin their ceremonies and rituals.