Sentences with phrase «in different cell lines»

The overall score encompass several factors: reproducibility of the antibody staining in different cell lines (and if signal strength correlates with RNA expression levels); assays for enhanced antibody validation by using antibodies binding to different epitopes on the same target protein (independent antibodies), by knockdown / knockout of the target protein (genetic methods) and by matching of the signal with a GFP - tagged protein (recombinant expression); experimental evidence for location described in literature.

In the first step, stainings in different cell lines with the same antibody are reviewed and the results are compared with available protein / gene characterization data for subcellular location.

The results, although still preliminary in a quantitative sense, demonstrate that the reactivation of methylation - silenced genes does occur in different cell lines, suggesting it is a general phenomenon.

By functionally linking the signal transduction of melanopsin to the control circuit of the nuclear factor of activated T cells, we have designed a synthetic signaling cascade enabling light - inducible transgene expression in different cell lines grown in culture or bioreactors or implanted into mice.

Not exact matches

The scientists also generated a panel of (reconstructed) ancestral and existing TRIM5 genes (19 total), expressed them in cultured cell lines, and exposed the cells to 16 different retroviruses (lentiviruses and others) to see which TRIM5 versions conferred resistance to which viruses.

But in many cell lines and patient samples that developed resistance to the combination therapy, the researchers observed something different happening.

The work, funded by the US National Human Genome Research Institute, aims to create human cell lines with subtly different genomes in order to test ideas about which mutations cause disease and how.

As those cells proliferated in laboratory dishes, the bits of human DNA were also copied, creating cell lines, each of which had a different fragment.

DNA from the cell nucleus, which includes both maternal and paternal lines, tells a different story: The two types of elephants are in fact separate species, leading recently to a proposed relisting of the forest elephant as Loxodonta cyclotis.

The enzyme was tested in three different cell lineages: leukemia cells incapable of producing asparagine at normal levels (MOLT4); another line of leukemia cells, in this case capable of producing asparagine at normal levels (REH); and non-malignant cells, used as a control (HUVECs).

Then, they produced the hemagglutanin with different combinations of these mutations in an experimental cell line (testing the mutations in H7N9 viruses themselves could be dangerous).

Even then, with a completely clean lab highly focused on meticulous technique, a new contamination struck in 2013 — something Myers identified when several different cell lines showed up with exactly the same mutation.

Further experiments showed that each iPS cell line had a different pattern of DNA methylation, a molecular coating of the DNA that turns genes on or off in the cell.

Several different experiments localized the active gene and its protein to stem cells buried within the crypts, or deep grooves in the intestinal lining.

Given their similar profiles, Seyedsayamdost and coworkers hypothesized that TDA might exhibit anticancer properties as well, and indeed observed its strong anticancer activity in a screen against 60 different cancer cell lines.

In a paper published online yesterday in Stem Cells, the researchers report that they succeeded in generating pluripotent human ES cell lines — i.e., cells that can develop into many different kinds of cells — from one of the 13 late - arrested embryoIn a paper published online yesterday in Stem Cells, the researchers report that they succeeded in generating pluripotent human ES cell lines — i.e., cells that can develop into many different kinds of cells — from one of the 13 late - arrested embryoin Stem Cells, the researchers report that they succeeded in generating pluripotent human ES cell lines — i.e., cells that can develop into many different kinds of cells — from one of the 13 late - arrested embCells, the researchers report that they succeeded in generating pluripotent human ES cell lines — i.e., cells that can develop into many different kinds of cells — from one of the 13 late - arrested embryoin generating pluripotent human ES cell lines — i.e., cells that can develop into many different kinds of cells — from one of the 13 late - arrested embcells that can develop into many different kinds of cells — from one of the 13 late - arrested embcells — from one of the 13 late - arrested embryos.

The researchers have isolated the sesquiterpene lactone damsin from the plant and studied its effect on cancer stem cells in three different breast cancer cell lines.

Part of the trouble is the ingredients: Subtle variations in tissue - culture chemicals and Matrigel, or in different stem cell lines and how they are grown first in 2 - D culture, can have a big impact on how the organoids turn out, Novitch says.

Multi-colored metastasis in the peritoneal lining of the abdomen comprised of red and yellow fluorescent cells demonstrating that pancreatic cancer spreads through interactions between different groups of cells.

The team used five types of antibiotics — including one used to treat acne (doxycycline)-- on cell lines of eight different types of tumour and found that four of them eradicated the cancer stem cells in every test.

«Our partners in the US took 1000 Genomes Project cell lines and treated them with different compounds, so we knew which compound had a toxic effect for each cell line,» explains Julio Saez - Rodriguez, former Research Group Leader at EMBL - EBI now at RTWH Aachen University.

«We've seen this in six different types of human cancer cell lines in the lab,» Choudhury said.

In experiments with cancer cell lines, the PIM1 inhibitors killed cells in a MYC - dependent manner, and in two different mouse models — one in which mice were implanted with patient tumors and the other in which a genetic alteration of MYC predisposes the mice to tumor development — the administration of PIM1 inhibitors resulted in significant tumor regressioIn experiments with cancer cell lines, the PIM1 inhibitors killed cells in a MYC - dependent manner, and in two different mouse models — one in which mice were implanted with patient tumors and the other in which a genetic alteration of MYC predisposes the mice to tumor development — the administration of PIM1 inhibitors resulted in significant tumor regressioin a MYC - dependent manner, and in two different mouse models — one in which mice were implanted with patient tumors and the other in which a genetic alteration of MYC predisposes the mice to tumor development — the administration of PIM1 inhibitors resulted in significant tumor regressioin two different mouse models — one in which mice were implanted with patient tumors and the other in which a genetic alteration of MYC predisposes the mice to tumor development — the administration of PIM1 inhibitors resulted in significant tumor regressioin which mice were implanted with patient tumors and the other in which a genetic alteration of MYC predisposes the mice to tumor development — the administration of PIM1 inhibitors resulted in significant tumor regressioin which a genetic alteration of MYC predisposes the mice to tumor development — the administration of PIM1 inhibitors resulted in significant tumor regressioin significant tumor regression.

There are many hundreds, if not thousands, of different stem cell lines in use around the world.

We found that the U87MG cell line from ATCC had a different DNA profile than the original cell line in Uppsala», says Bengt Westermark.

In the different cell types, the loops begin and end at different specific chromosomal locations, so each cell line's genome appears to have a unique population of loops.

The study used a well - known line of pancreatic cancer cells (AsPC - 1) in the laboratory and assessed how well this grew when treated with either the chemotherapy drug gemcitabine or different levels of commercially available chokeberry extract alone, and when treated with a combination of gemcitabine and chokeberry extract.

Cell lines are therefore indispensable in medical research and a large number of cell lines exist that originate from many different tumor tyCell lines are therefore indispensable in medical research and a large number of cell lines exist that originate from many different tumor tycell lines exist that originate from many different tumor types.

Moreover, cell lines vary in their ability to make different tissues, such as heart muscle or cartilage, so a suite of clinical - grade lines is needed, Braude says.

When the team split this cell line into different dishes, sometimes the donor egg's mtDNA won out; but in others, the carried - over mtDNA dominated.

In principle, tissue banks of parthenogenetic cell lines could include enough different immune protein combinations to treat up to half of the U.S. population — men as well as women — Lanza says.

In parthenote - derived cell lines, they found a distinctive signature: Most genes were different on the two sets of chromosomes, but genes located close to the chromosome's center were largely identical.

The scientists were thus able to transfer the genetic information from the known fluorescent protein eqFP615 into the DT40 chicken B cell line in order to produce protein variants of the new infrared marker Amrose with different spectral properties.

The generality of this observation was further confirmed by examining the expression of miR - 7 in 2 different cell lines, CN34 and MCF7, and their metastatic variants, CN34BoM, CN34BrM, and MCF7BoM.

We capitalized on one of the innate responses of microvascular endothelial cells, i.e., the up - regulation of cell adhesion molecules in response to inflammatory agents, to generate endothelial cell lines from different organs.

The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in generating microvascular endothelial cell lines from a number of different organs.

C, miR - 7 expressions in both parental cells and CSCs from different cell lines were measured by TaqMan PCR.

However, efforts to examine the in vitro behavior of tumor cells with endothelial cells from different anatomical regions have been prohibited because, in large part, of the limited number of organ - derived endothelial cell lines available for study.

The resulting endothelial cell lines described in this study retain their phenotype after repeated passage, require minimal nutritional support and, importantly, may be useful for identifying factors that regulate endothelial cell proliferation and tumor cell adhesion in different anatomical regions.

The immortalized endothelial cell lines established from H - 2Kb - tsA58 mice provide, for the first time, a cell culture system to examine factors regulating angiogenesis and tumor cell arrest in different organ systems.

These stem cells live in the lining of the intestine, known as the epithelium, and generate all of the different cell types that make up the epithelium.

In other studies, however, using cell lines either in vivo or in vitro suggest that the alpha2 beta1 integrin may serve to enhance metastasis to different organIn other studies, however, using cell lines either in vivo or in vitro suggest that the alpha2 beta1 integrin may serve to enhance metastasis to different organin vivo or in vitro suggest that the alpha2 beta1 integrin may serve to enhance metastasis to different organin vitro suggest that the alpha2 beta1 integrin may serve to enhance metastasis to different organs.

To create these cells, we terminally differentiated three hiPS cell lines (ChiPSC12, ChiPSC18, and ChiPSC22; abbreviated C12, C18, and C22) from different individuals into three lines of Cellartis enhanced hiPS - HEP cells; one of these lines is included in each complete kit.

For this purpose, at least 30 different cell lines derived from biopsies (HNSCC — head and neck cancer — patients) wil be collected and grown in culture to esstablish cell lines.

The goal was to deepen the knowledge about the pharmacological and cytotoxic effects of the drug Temodex in vitro in different human brain cancer cell lines.

Originally three cell lines, U-2 OS, A-431 and U-251 MG, originating from different human tissues were chosen to be included in the immunofluorescent analysis.

To directly evaluate the relationship between ABL family kinases and metastasis, we analyzed ABL1 and ABL2 protein abundance in MDA - MB - 231 — derived breast cancer cell lines with different organ metastasis tropisms (19).

The effect of a novel antagonist of growth hormone releasing hormone on cell proliferation and on the key cell signaling pathways in nine different breast cancer cell lines.

In contrast, no difference was observed in the sensitivity of these cell lines to cytosine arabinoside (ARA - C), a standard chemo - therapeutic drug which targets different pathways (data not shownIn contrast, no difference was observed in the sensitivity of these cell lines to cytosine arabinoside (ARA - C), a standard chemo - therapeutic drug which targets different pathways (data not shownin the sensitivity of these cell lines to cytosine arabinoside (ARA - C), a standard chemo - therapeutic drug which targets different pathways (data not shown).

We use this in vitro model of spontaneous tumorigenesis to generate a library of cell lines with 25 different de novo JAK1 - activating mutations, 6 of which are targeting two residues, Phe958 and Pro960, located in the hinge region at the periphery of the ATP - binding pocket.