With advances in spatial resolution reaching the atomic scale, two - dimensional (2D) and 3D
imaging in electron microscopy has become an essential methodology in various fields of study.
In this Review, we highlight recent developments and inventions made by introducing the fourth dimension of
time in electron microscopy.
Research problems that are just out of reach today but that could be made accessible by
advances in electron microscopy include studies of the little pores that form in our cells walls and which are centrally important in the regulation of all life processes as well as new nano - structured materials that are ultra-light yet strong, allowing reduced energy consumption in vehicles.
Joachim Frank received his Nobel Medal on Dec. 10 in Stockholm and described his work
in electron microscopy in his Nobel lecture.
They also utilized immunogold labeling — in which gold particles are attached to antibodies that bind to particular proteins in order to make them
visible in electron microscopy — to show that filaments within the feathers were keratin.
Carol Mason, PhD, another Zuckerman Institute principal investigator, contributed
expertise in electron microscopy, a powerful technology that allows individual cells to be observed in high resolution.
Brookhaven physicist Qiang Li (right) and materials scientist Lijun
Wu in an electron microscopy lab in the Condensed Matter Physics and Materials Science Department at Brookhaven Lab.
The researchers gathered single cells of warnowiids off the coasts of B.C. and Japan, sequenced their genomes, and analyzed how the eyes are built using new
methods in electron microscopy that allow the reconstruction of three dimensional structures at the subcellular level.
I had obtained a master?s degree in physiology years earlier, and the technician job had been created to utilize my specialized
skills in electron microscopy.
Taking advantage of the latest
advances in electron microscopy and computer - controlled imaging, Seung and his team are creating some of the most detailed three - dimensional reconstructions of cortical gray matter ever made.
Advances
in electron microscopy and native mass spectrometry enabled the researchers to determine that a critical component of the signaling system, anchored protein kinase A, remains intact during activation.
Advances
in electron microscopy had also enabled a resolution of a few nanometres to be reached, although this was based on high - energy electron beams that could damage the materials being studied.