Sentences with phrase «in embryonic stem»

It has also been reported that Kap1 controls endogenous retroelements during early embryonic development and in embryonic stem cells but not in adult fibroblasts [45].

Lineage commitment in the mammalian embryo is most often depicted as a series of binary choices between alternate cell states, and increasing evidence supports the hypothesis that fate decisions in embryonic stem (ES) cell cultures reflect these developmental processes [1].

The «Functional Genomics in Embryonic Stem Cells» consortium comprising 20 research groups (acronym FunGenES; http://www.fungenes.org) has analyzed the transcriptome of ES cells under a series of diverse stimuli during growth expansion and differentiation.

Human NIAM is involved in chromosome segregation, p53 regulation and cell proliferation in somatic cells, but its role in embryonic stem cells is unknown.

Commitment in embryonic stem cells is often depicted as a binary choice between alternate cell states, pluripotency and specification to a particular germ layer or extraembryonic lineage.

To investigate gene networks operating in pluripotent ES cells and their derivatives, the «Functional Genomics in Embryonic Stem Cells» consortium (FunGenES) has analyzed the transcriptome of mouse ES cells in eleven diverse settings representing sixty - seven experimental conditions.

This study confirms that in embryonic stem cells Myst2 is part of H3 and H4 histone acetylation complexes similar to those described in somatic cells.

Mll2 is required for H3K4 trimethylation on bivalent promoters in embryonic stem cells, whereas Mll1 is redundant.

This collaboration also showed that Gcn5 is important for Myc - mediated stem cell self - renewal in embryonic stem cells.

We replaced exon 3, which encodes a part of the RNA binding domain and is present in all Boule isoforms (Figure 3C), with a lacZ - neo vector through homologous recombination in embryonic stem cells.

We have a particular focus on glycosaminoglycans and have studied the role of heparan sulphate in embryonic stem cell behavior.

These are the same four genes that seemed to be very active in embryonic stem cells.

The scientists had strategically inserted these foreign DNA «markers» at particular points along the genome, next to genes expressed only in embryonic stem cells.

Publication:» Histone H3.3 is required for endogenous retroviral element silencing in embryonic stem cells», Simon J. Elsässer, Kyung - Min Noh, Nichole Diaz, C. David Allis, Laura A. Banaszynski, Nature, online April 4, 2015, DOI: 10.1038 / nature14345.

Further, we demonstrate that deletion of Alkbh1 in embryonic stem cells leads to upregulation of the core genes involved in pluripotency and that ALKBH1 is required during early differentiation.

To determine whether huntingtin is involved in trafficking a specific subset of proteins, we measured changes in global transcription levels in embryonic stem cells and neurons lacking huntingtin.

Initial analysis found a significant enrichment of PRC1 / 2 targets in hypermethylated promoters in bCSCs, similar to that observed in embryonic stem cells (ESCs)[2].

The creation of iPSCs began by studying proteins not only uniquely expressed in embryonic stem cells, but proteins known to be functionally important in creating the unique properties of these cells.

He studies post-transcriptional regulations during oocyte - to - zygote transition and the role of small RNAs in embryonic stem cells.

Paired SAGE - microarray expression data sets reveal antisense transcripts differentially expressed in embryonic stem cell differentiation.

Lane believes that research in embryonic stem cells will likely be short - lived, as scientists learn what they need to know about the function of cells.

Suppose you run a ChIP - seq experiment (think «GPS for proteins») for NANOG, an essential transcription factor in embryonic stem cells (ESCs).

Title: Interactions between Notch - and hypoxia - induced transcriptomes in embryonic stem cells Authors: Main H, Lee KL, Yang H, Edgren H, Sahlgren C, Jin S, Kallioniemi O, Poellinger L, Lim B and Lendahl U Date: 2010 Publication Details: Exp Cell Res.

We'll start with ChIP - seq data from the ENCODE project, find enhancers in embryonic stem cells, and conclude with a targeted CRISPR screen that can disrupt these enhancers.

When the studies of mitochondrial carryover were done in embryonic stem cells derived from ePNT blastocysts (to allow for more cell divisions during which instability could manifest), some stem cell lines displayed instability and an increase in mitochondrial DNA carryover with time.

Efficient production of mesencephalic dopamine neurons by Lmx1a expression in embryonic stem cells

It will appeal to those interested in adult stem cells as well as those interested in embryonic stem cells.

FoxP1 promotes midbrain identity in embryonic stem cell - derived dopamine neurons by regulating Pitx3

BRD7, a novel PBAF - specific SWI / SNF subunit, is required for target gene activation and repression in embryonic stem cells.

Scientists investigating the mechanisms of Down Syndrome (DS) have revealed the earliest developmental changes in embryonic stem cells caused by an extra copy of human chromosome 21 — the aberrant inheritance of which results in the condition.

Tensions are growing in Berlin ahead of a parliamentary vote that medical scientists say will determine whether or not Germany can continue to participate effectively in embryonic stem cell research.

(D) RT - PCR detection of mRNA expression originating from the targeted † XP and † XPCS alleles in embryonic stem cell clones using primers F1 (hybridising outside the targeting construct) and mR as indicated in (A) results in a 1,416 - bp fragment.

mRNA expression from the targeted allele could be detected in embryonic stem cells by RT - PCR (Figure 1D), although expression was reduced approximately 5-fold relative to wt mRNA transcript levels as determined by Northern blotting of RNA from the testis of heterozygous animals (Figure 1E).

Integration of external signaling pathways with the core transcriptional network in embryonic stem cells.

N. Yasui et al., «Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation,» Molecular Cell, doi: 10.1016 / j.molcel.2014.05.002, 2014.

Research led by the Babraham Institute with collaborators in the UK, Canada and Japan has revealed a new understanding of how an open genome structure supports the long - term and unrestricted developmental potential in embryonic stem cells.

Previous research has shown that heterochromatin is maintained in an unusually open and uncompacted organisation in embryonic stem cells, which is different to all other cell types.

The study, «Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation,» was supported by the National Institutes of Health.

They hope to identify the complete set of DNA binding sites and corresponding target genes for the regulons in embryonic stem cells and a subset of the cells they differentiate into.

But Mary Herbert, a reproductive biologist at the University of Newcastle, UK, who is part of a team pursuing mitochondrial replacement, says that mitochondria behave very differently in embryonic stem cells compared to normal human development.

This Korean researcher racked up a series of important advances in embryonic stem cell technology, including the first lines of cells from patients

«Long - held assumption of gene expression in embryonic stem cells challenged.»

«We studied how the Sox2 gene is turned on in mice, and found the region of the genome that is needed to turn the gene on in embryonic stem cells,» said Professor Jennifer Mitchell of U of T's Department of Cell and Systems Biology, lead invesigator of a study published in the December 15 issue of Genes & Development.

Mitchell and her colleagues eliminated this possibility when they deleted these nearby regions in the genome of mice and found there was no impact on the gene's ability to be turned on in embryonic stem cells.

Yamanaka's group and two others followed up earlier this year with firmer evidence that these induced pluripotent stem (iPS) cells faithfully mimicked the patterns of gene activity and cellular differentiation observed in embryonic stem cells.

So researchers began testing genes that were only active in embryonic stem cells to try to pin down those capable of triggering the change.

The only previous demonstration — by researchers led by Shou - Wei Ding, PhD, a professor of Plant Pathology and Microbiology at UC Riverside and co-corresponding author of the current study — was done in embryonic stem cells and in newborn mice.

Researchers called them induced pluripotent cells, which are created by enticing cells to turn on genes normally found in embryonic stem cells.

Base oxidation regulates gene activity In cooperation with colleagues at LMU, as well as researchers based in Berlin, Basel and Utrecht, Carell and his group have now shown, for the first time, that a standard base other than cytosine is also modified in embryonic stem cells of mice.

It can be used in embryonic stem cell research, or in regenerative medicine where it is sometimes referred to as «therapeutic cloning.»