Sentences with phrase «in endothelial cell density»

This study examines long - term changes in endothelial cell density in eyes implanted with Artisan (Ophtec BV) iris - fixated phakic intraocular lens.

We considered focusing our studies on the peripheral corneal endothelium and endothelial limbus after reports by Schimmelpfenning [16] and Daus et al. [17] indicated a significant increase in endothelial cell density of the peripheral corneal endothelium.

Not exact matches

Endothelial cells (passage 5) from each line were plated onto 96 - well plates at a density of 1000 cells / well in 10 % DMEM.

Matrigel - containing plates were allowed to incubate at 37 °C for 30 min and then seeded with endothelial cells at a density of 4 × 104 cells / well in 10 % DMEM.

Endothelial cells plated onto two - chamber slides at a density of 2 × 105 cells / well in 10 % DMEM were incubated overnight.

In B, endothelial cells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldehydIn B, endothelial cells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldecells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldecells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldehydin two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldehydin 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldeCells were washed with label - free medium and fixed in 4 % paraformaldehydin 4 % paraformaldehyde.

Increased endothelial cell density in the paracentral and peripheral regions of the human cornea.

Morphologically similar endothelial cells have been observed when they were differentiated into mature endothelial (CD133 --RRB- by culturing them in high density culture from low density culture at different passages.

The low density culture protocol helps in generating larger number of CD133 + endothelial population from initially fewer CD133 + endothelial progenitor cells; EPCs (Figure S1).