Sentences with phrase «in fibroblast cell»

One of the many payoffs of the International HapMap Project was that it characterized genetic variation in fibroblast cell lines that could be ordered from Coriell for subsequent experiments.

Special focus is placed on Rho - kinase inhibition, relating to its potential to promote and support extracellular matrix production in cultured chondrocytes and its role in fibroblast cells as a part of direct chemical cellular differentiation.

To further facilitate genome editing in pigs, we report here establishment of a pig line with Cre - inducible Cas9 expression that allows a variety of ex vivo genome editing in fibroblast cells including single - and multigene modifications, chromosome rearrangements, and efficient in vivo genetic modifications.

Not exact matches

In a preliminary experiment, the team found that stem cells produced from fibroblasts of men with Klinefelter syndrome also lost the extra sex chromosome.

They turned the fibroblasts into stem cells and noticed that in the process, some of the cells lost the extra sex chromosome.

Ueli Schibler of the University of Geneva showed in 1998 that the per genes of rat connective - tissue cells called fibroblasts are active according to a circadian cycle.

Working with human breast tissue, the new study's authors attempted to induce EMT in normal cells; they figured they would just get fibroblasts, a type of connective tissue that is important in wound healing.

Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3 / 4, Sox2, and Klf4 and the other containing the c - Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras.

A team led by Byeong - Chun Lee of Seoul National University in South Korea created the dogs by cloning fibroblast cells that express a red fluorescent gene produced by sea anemones.

The production of virus - free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine.

Ding's team took cells called fibroblasts from the connective tissues of mouse fetuses and bathed them in a cocktail of the four polyarginine - tagged proteins for 12 hours, then they removed the reprogramming proteins for 36 hours, and repeated this cycle four times over.

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

«Fibroblast growth factor receptor inhibitors are new therapies being developed in clinical trials for patients whose cancer cells have genetic alterations in this family of genes,» says Roychowdhury, a member of the OSUCCC — James Translational Therapeutics Program.

Conventional, high - dose chemotherapy treatments can cause the fibroblast cells surrounding tumors to secrete proteins that promote the tumors» recurrence in more aggressive forms, researchers at Taipei Medical University and the National Institute of Cancer Research in Taiwan and University of California, San Francisco, have discovered.

It takes far more expertise, however, to remove dozens of PERV genes at once, as eGenesis does in pig fibroblasts, which are connective - tissue cells.

The researchers found that, in response to the maximum tolerated doses of several commonly used chemotherapy drugs, breast cancer - associated fibroblasts secrete large amounts of cell signaling proteins called ELR + chemokines.

Amounts of telomerase RNA increased in immortal cells derived from primary mouse fibroblasts.

To find out, John O'Neill, a biologist at the Medical Research Council's Laboratory of Molecular Biology in Cambridge, U.K., and his team studied skin cells known as fibroblasts, which are essential for wound healing.

The «target» cells on the other side of the BeWo barrier to the nanoparticles were human fibroblast cells, found in skin and connective tissue.

When compounds that block the «message» channels in cell membranes were added to the dish, there was no damage to fibroblasts.

Avivi's team has found out that fibroblast skin cells from the armpits of the rats can kill human cancer cells in a dish.

When cells that produced IL - 21 were put in culture with synovial fibroblasts (which are the main contributors to joint inflammation in rheumatoid arthritis), they induced the production of proinflammatory cytokines by these synovial fibroblasts, and cells that do not produce IL - 21, did not demonstrate this same outcome.

The study team removed fibroblasts (skin cells) from DBA patients, and in cell cultures, using proteins called transcription factors, reprogrammed the cells into iPSCs.

Importantly, the new technique grows the pluripotent stem cells as floating spheres in high concentrations of two growth factors, fibroblast growth factor - 2 and epidermal growth factor.

Two months after therapy, tissue analysis showed less scarring and higher levels of regenerative agents such as fibroblast growth factor — which accelerate healing — in rats treated with SIS plus stem cells compared with those treated with SIS alone.

In studies with human fibroblasts that make up connective tissue, Boger's team tested whether NOD1 activity could affect CMV replication in cultures of cells grown in the laIn studies with human fibroblasts that make up connective tissue, Boger's team tested whether NOD1 activity could affect CMV replication in cultures of cells grown in the lain cultures of cells grown in the lain the lab.

To see if the same chain of events happens in rheumatoid arthritis, Smith's team tested whether various kinds of fibroblasts would spur T cell migration in test tubes.

In a related paper published online today in Nature Biotechnology, Konrad Hochedlinger of the Harvard Stem Cell Institute in Cambridge and his colleagues compared the gene expression patterns in mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tipIn a related paper published online today in Nature Biotechnology, Konrad Hochedlinger of the Harvard Stem Cell Institute in Cambridge and his colleagues compared the gene expression patterns in mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tipin Nature Biotechnology, Konrad Hochedlinger of the Harvard Stem Cell Institute in Cambridge and his colleagues compared the gene expression patterns in mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tipin Cambridge and his colleagues compared the gene expression patterns in mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tipin mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tips.

They start by harvesting skin cells known as fibroblasts and growing these in a sheet.

To find out, Deb and his co-authors genetically tagged cardiac fibroblasts in mice and watched as they transitioned into bone - forming, osteoblast - like cells after heart injury.

So Welham's team set out to engineer vocal fold mucosae in the lab using the two main cell types that make up the tissue — connective fibroblasts, which form the main body, and epithelial cells, which line the surface.

The researchers found that fibroblasts and Hofbauer cells were susceptible to infection by Zika virus in isolated cultures.

Hamlyn, working with Richard Schmidt of the School of Pharmacy at the University of Wales in Cardiff, exposed human fibroblast cells to six fungi strains.

For many years, tattoos were thought to work by staining fibroblast cells in the dermal layer of the skin.

It spurred the fastest growth of fibroblast cells, with 17 per cent more cells formed after six days as in a control culture.

A study by Mo's group in a 2011 issue of Experimental Biology and Medicine (236:604 - 613) showed that normal fibroblasts are 10-fold more resistant than murine B16 melanoma cells to geranylgeraniol - mediated growth suppression.

The tissue, called the stroma, includes fat cells, or adipocytes, that provide padding; fibroblasts, which make the framework for tissue; pericytes in blood vessels, which are contractile cells that help regulate blood pressure; as well as myoepithelial cells comprising the outer layer of the ductal system through which milk flows.

«We also showed for the first time that AHR signaling in primary human orbital fibroblasts disrupts the pro-fibrotic Wnt / β - catenin pathway, which is important for scar cell formation,» noted Dr. Phipps.

The orbital remodeling in TED is likely induced by infiltrating T lymphocytes and mast cells, which activate orbital fibroblast effector cells to either proliferate and form scar - producing cells called myofibroblasts or turn into pro-inflammatory fat cells.

The experiments, including studies conducted in a bioengineered 3 - D model of lung tissue seeded with relevant cells, showed that CHI3L1 regulates a pathway that recruits cells such as macrophages and fibroblasts that produce the scarring, or fibrosis.

In the online edition of Science, he and his colleagues report that Oct4 and Sox2 were capable of converting neonatal foreskin fibroblasts into cells similar to Yamanaka's, whereas Nanog significantly boosted the frequency of reprogramming and Lin28 upped it by a moderate amount.

Skin - producing cells called fibroblasts from the tip of an adult mouse's tail have been reprogrammed to make eggs, Japanese researchers report online October 17 in Nature.

Now Yamanaka and his colleagues report in the journal Cell that the same combination of genes induced pluripotency in commercially available human fibroblasts (connective tissue cells that play a crucial role in healing) derived from the facial skin of a 36 - year - old woman, the joint tissue of a man, aged 69, and a newborn, respectively.

Cells called cancer - associated fibroblasts arrange a normal meshwork of fibers into straight tracks, cell biologist Begum Erdogan of Vanderbilt University in Nashville and colleagues reported December 13 at the annual meeting of the American Society for Cell Biolcell biologist Begum Erdogan of Vanderbilt University in Nashville and colleagues reported December 13 at the annual meeting of the American Society for Cell BiolCell Biology.

Fibroblasts (red) express endothelial markers (green), making the heart cells in mice appear yellow.

To explore this idea, they induced heart attacks in mice and then studied the fibroblasts to see if the cells expressed markers characteristic of endothelial cells.

He says that the extracts from their fibroblasts do contain the sticky substance, but that in spalacids it does not seem to play a key role in the animals» natural resistance to cancer or activity against cancer cells in the lab.

When this article was first published on 15 August 2013, it did not explain that HMM - MA in the fibroblasts of Middle East mole rats does not seem to be significant in their resistance to cancer or activity against cancer cells.

Imagine researchers» excitement, then, when Kyoto University's Shinya Yamanaka reported last year that his group had created embryolike cells directly, without transferring chromosomes, simply by injecting mouse fibroblast cells with four genes that are active in embryonic cells but not in adult ones.

- catenin pathway activates certain lncRNAs to directly control gene expression in skin fibroblast cells.»