Across all four cancer types, the new method of selecting candidate genes based on inter-tumor variation
in gene expression outperformed the other methods, including the standard method of comparing mean expression in adjacent normal and tumor tissues.
What we were surprised to find out was that the real differences we could detect
in terms of when we did the swap experiments to say which yeast could
outperform the other — what we learned was that the GAL1
gene, that the part [of] that, the DNA sequence is outside of the GAL1
gene, it acts as a switch to turn up or turn down GAL1
expression, that had evolved considerably from the ancestral situation; and same for the GAL3.And then what had happened was that each function had been optimized, that GAL3 had sort have been tuned to be sort of a loosely regulated kind of available anytime sensor of galactose and GAL1 had evolved to be an incredibly tightly regulated,
in fact, it's the most tightly regulated
gene you know of
in yeast.