RT - PCR and in situ hybridization confirmed the presence of 5 - HT3A mRNA
in the geniculate ganglion.
In our study, pentobarbital inhibited 5 - HT evoked calcium signals
in geniculate ganglion neurons and, at anesthetic levels in mice, significantly inhibits 5 - HT3 function in vivo.
RT - PCR shows 5 - HT3A transcripts
in geniculate ganglia as well as in brain (Fig. 1A).
In mice expressing GFP under the control of the 5 - HT3A promoter, a subset of cells
in the geniculate ganglion and nerve fibers in taste buds are GFP - positive.
Consistent with GFP expression
in the geniculate ganglion, most taste buds innervated by the geniculate ganglion contained some GFP - labeled intragemmal nerve fibers.
A, RT - PCR showing the presence of 5 - HT3A transcripts in brain and geniculate ganglion of WT mice, but the lack of 5 - HT3A mRNA
in geniculate ganglion of 5 - HT3AKO mice.
They hooked a potential receptor, serotonin 3, to green fluorescent protein and showed that the nerve terminals in the mouse tongue have serotonin 3 receptors, and these receptors are made by the nerve cells located
in the geniculate ganglion.
At lower levels, in the retina and
in the geniculate, cells in these two subdivisions differ in their color selectivity, contrast sensitivity, temporal properties, and spatial resolution.
Not exact matches
It now looks like they have their lateral
geniculate nucleus (LGN)-- part of the thalamus
in the middle of the brain — to thank for this «blindsight».
When they tracked the cells» axons from the eye into the brain, they saw that many of them terminated
in the lateral
geniculate nucleus (LGN), the first relay station
in the brain for visual information.
Specifically, they have found evidence of processing
in the human lateral
geniculate nucleus (LGN), a small node
in the thalamus
in the middle of the brain that relays nerve impulses from the retina to the primary visual cortex.
In both primates and rodents, messages from the two eyes enters the brain through a small structure called the lateral geniculate nucleus or LGN, which is made of slivers of nerve cells, arranged like sponge in a layer cak
In both primates and rodents, messages from the two eyes enters the brain through a small structure called the lateral
geniculate nucleus or LGN, which is made of slivers of nerve cells, arranged like sponge
in a layer cak
in a layer cake.
C, D, Ca2 + signals from GFP +
geniculate ganglion cells
in response to 10 μm ATP, 10 μm ATP + 1 μm ODS, 10 μm 5 - HT, 10 μm 5 - HT + 1 μm ODS, and 55 mm KCl (C) or 10 μm ATP, 10 μm CPG, 10 μm CPG + 1 μm ODS, and 55 mm KCl (D).
Approximately 25 % of all WT
geniculate neurons (5/19; Fig. 5) responded to exogenous 5 - HT with an increase
in intracellular calcium, whereas no neurons from 5 - HT3AKO mice responded (0/25, p = 0.011, Fisher's exact test; Fig. 5).
Geniculate ganglia were rapidly excised from CO2 - euthanized mice and placed
in enzyme solution [minimum essential medium with Earle's balanced salts (MEM / EBSS; Hyclone) containing 1.25 mg / ml trypsin (Sigma - Aldrich) and 2.5 mg / ml collagenase A (Roche Diagnostics)-RSB- for 30 min.
In situ hybridization on sections of gustatory ganglia (
geniculate, petrosal / nodose) and a somatosensory ganglion (trigeminal) were performed to reveal serotonin receptor gene expression at the cellular level.
Somatosensory innervation of these taste fields arises from the trigeminal nerve; therefore, cells of the
geniculate ganglion that innervate taste fields are gustatory rather than somatosensory
in function.
Interestingly, when nonbarbiturate anesthetics are used, a concentration - dependent increase
in sideband excitation is observed
in a subset of
geniculate ganglion neurons (Wu et al., 2015).
GFP immunoreactivity was detected
in a subset of
geniculate ganglion neurons (169/682, 24.8 %; Fig. 2B, C), whereas the P2X3 signal was present
in nearly all ganglion cells (664/682, 97.4 %; Fig. 2B, C).
To first determine whether gustatory afferents express GFP
in 5 - HT3AGFP mice, we examined the
geniculate ganglion and taste buds.
In addition, we tested whether other 5 - HT receptors were upregulated after genetic deletion of 5 - HT3 by measuring 5 - HT responses in isolated geniculate ganglion neurons of WT and 5 - HT3AKO mic
In addition, we tested whether other 5 - HT receptors were upregulated after genetic deletion of 5 - HT3 by measuring 5 - HT responses
in isolated geniculate ganglion neurons of WT and 5 - HT3AKO mic
in isolated
geniculate ganglion neurons of WT and 5 - HT3AKO mice.
Here the axons going down the optic nerve, across the optic chiasm, and into the expected targets
in the brain: the lateral
geniculate and the superior colliculus.
The anterograde (forward) tracing results of retinal projections
in the garden warbler demonstrated virtually identical connections between the eye and the brain as known from other lateral - eyed bird species [e.g. 15]--[18]: fibers either projected onto the contralateral optic tectum (part of the tectofugal system), to the nucleus of the basal optic root (part of the accessory pathway)(data not shown) or curved into the thalamus innervating the dorsal
geniculate complex (Gld, for details, see Fig. 1C, 2A, D).