Sentences with phrase «in human breast cancer»

Should collaborations like these prove to be important also in human breast cancer, disrupting key interactions may be a novel treatment strategy for the disease.

We further confirmed that consistent with the results of the human breast cancer cell lines (Figure 2), the protein level of APC was elevated in the human breast cancer cells isolated from the anti-miR-142-3p-expressing breast cancer xenograft (Figure 7B).

We further confirmed that the protein level of APC was elevated in the human breast cancer xenograft cells transfected with the anti-miR-142-3p lentivirus (Figure 7B).

We further confirmed that the protein level of APC was elevated in the human breast cancer xenograft cells transfected with the anti-miR-142-3p-expressing lentivirus than those transfected with the control lentivirus in vivo (Figure 7B).

Using RNA interference (RNAi), first author Richard Possemato targeted these genes in human breast cancer cells implanted in mice.

This activity is a hot spot for mutations in human breast cancer patients,» says Inder Verma, Ph.D., the study's senior investigator.

MicroRNA - 34a suppresses cell proliferation by targeting LMTK3 in human breast cancer mcf - 7 cell line.

Previous studies reported that there is a reduction or loss of expression of the APC protein in 40.7 % of primary human breast cancers (Ho et al., 1999), and it appears to be more often due to promoter methylation (36 - 54 %) and loss of heterozygosity (LOH)(23 %) than to somatically acquired APC mutations in human breast cancers (Sarrio et al., 2003, Jin et al., 2001, Banerji et al., 2012, 2012).

It has also been found that a drug called megesterol (Megace), which is used in human breast cancer as well as a birth control product; medroxyprogesterone acetate (Depo - Provera) will also cause this disease to go into remission.

Additional experiments using a combination of maraviroc and a drug that blocks the VEGF protein suggest that the treatment duo could be an effective way to prevent metastatic disease in human breast cancer patients, according to the researchers.

He is researching the functions and properties of human sulfotransferase (EST) enzymes in human breast cancer cell lines.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

«If further studies validate that these processes are critical in human breast cancers,» Koshy notes, «the possibility exists that agents that favorably modify the biophysical properties of the extracellular matrix, or that target the receptors and signaling molecules associated with how cells sense this matrix, could be used as a new avenue for the prevention or treatment of breast cancers.»

The team is continuing with their experiments, to study the activity of this and other therapeutic ASOs in human breast cancer, with the hope of later moving the research into human clinical trials.

Differentially expressed proteins in human breast cancer cells sensitive and resistant to paclitaxel.

The analysis of doxorubicin resistance in human breast cancer cells using antibody microarrays.

We examined the regulation of activation of matriptase in human breast cancer cells, in comparison to non-transformed mammary epithelial cells 184A1N4 and MCF - 10A.

The role of enoyl - CoA hydratase short chain 1 and peroxiredoxin 3 in PP2 - induced apoptosis in human breast cancer MCF - 7 cells.

Ab7291 staining alpha tubulin in human breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).

Interleukin - 6 induces an epithelial - mesenchymal transition phenotype in human breast cancer cells.

Then, using semi-quantitative real - time PCR, we found that the expression of miR - 142 was able to upregulate the expression of miR - 150 in human breast cancer MDA - MB - 231 cells (Figure 5C).

We confirmed that the protein level of APC was elevated and the expression of miR - 150 was decreased in the human breast cancer xenograft cells transfected with the anti-miR-142-3p lentivirus (Figure 5E and Figure 7B).

A 2013 article published in the academic journal Food & Function notes that limonoids have anti-proliferative and anti-aromatase properties in human breast cancer cells.

Parabens can mimic estrogen, and have been detected in human breast cancer tissue.

In those cases, radiation therapy after or before the surgery often extends the period of time the cat remains in remission - just as it does in human breast cancer.

PIK3CA and p53 are the two most frequently mutated genes in human breast cancer and are associated with different molecular subtypes.

We further confirmed that the protein level of APC was elevated in the human breast cancer xenograft formed by the human BCSCs transfected with the anti-miR-142-expressing lentivirus (Figure 7B), which is consistent with our observations using the breast cancer cell lines.

The study, published in the Journal of the National Cancer Institute (JNCI), identified four genes that are linked to tamoxifen resistance and poor prognosis of breast cancer, by comparing results obtained in a new animal model, in human breast cancer cells grown in culture, and in publically available datasets collected from thousands of estrogen receptor positive breast cancer patients treated with tamoxifen.

«The interesting thing is that when we looked the same dog genes in human breast cancer, epigenetic aberrations occur in the same regions of DNA.

Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF - a and the NF -?

«We have found that the same genes responsible for tamoxifen resistance in our animals are also turned off in human breast cancer cells that do not respond to the drug,» she says «Because these genes were epigenetically silenced — meaning they were not irreversibly altered, just switched off — it was possible to turn them back on.

And we confirmed that the growth of the tumors formed by the human BCSCs transfected with the anti-miR-142-expressing lentivirus was significantly slower than those of the control tumors formed by the control lentivirus transfected BCSCs (Major points raised by the editors and the reviewers # 3) These data suggest that the regulation of APC and the Wnt signaling is at least one of the important pathways targeted by miR - 142 in human breast cancer cells and BCSCs.

1) The results of the inhibition of miR -142-3p in the human breast cancer xenograft cells and the Wnt signaling pathway inhibition in human breast cancer cells have confirmed that our finding that miR - 142 targets APC, activates the Wnt signaling pathway and upregulates miR - 150 is applicable to human breast cancers.