Sentences with phrase «in human cancer cell lines»

Azacytidine and Decitabine induce gene - specific and non-random DNA demethylation in human cancer cell lines

To validate their computer modeling predictions, researchers performed experiments in human cancer cell lines, mouse liver samples and primary human hepatocytes.

Not exact matches

Introducing human prostate cancer cell lines into mice, Wu and his colleagues saw a particular enzyme called MAOA activate a cascade of signals that made it easier for tumor cells to invade and grow in bone.

«This model was trained on genetic data from human tumors in The Cancer Genome Atlas and was able to predict response to certain inhibitors that affect cancers with overactive Ras signaling in an encyclopedia of cancer cell lines,» GreeneCancer Genome Atlas and was able to predict response to certain inhibitors that affect cancers with overactive Ras signaling in an encyclopedia of cancer cell lines,» Greenecancer cell lines,» Greene said.

Professor Ali Tavassoli, who led the study with colleague Dr. Ishna Mistry, explains: «In an effort to better understand the role of HIF - 1 in cancer, and to demonstrate the potential for inhibiting this protein in cancer therapy, we engineered a human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environmenIn an effort to better understand the role of HIF - 1 in cancer, and to demonstrate the potential for inhibiting this protein in cancer therapy, we engineered a human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environmenin cancer, and to demonstrate the potential for inhibiting this protein in cancer therapy, we engineered a human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environmenin cancer therapy, we engineered a human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environmenin a hypoxic environment.

The researchers observed the effect of the synthetically produced molecule, JK - 31, on the growth and proliferation of a model human breast cancer cell line and found that it effectively blocked the protein cyclin - dependent kinase 1 (CDK1), which plays a key part in the process of the division of cancer cells, and therefore inhibited the proliferation of the cells.

They found out that TiY is capable of distinguishing TICs from non-TICs in various human lung cancer cell lines and patient - derived lung tumors.

Beyond lung cancer, TiY is able to target TICs in 28 types of human cell lines derived from the central nervous system, melanoma, breast, renal, ovarian, colon, and prostate cancer.

«Our study results suggest a new drug cocktail that is effective in both human lung cancer cell lines and fly models,» says Cagan.

Through these effects, the PERY peptide reduced the proliferation of several (but not all) cancer cell lines in culture and inhibited the growth of a human breast cancer xenograft in mice.

In earlier studies involving animal models and human cancer cell lines, researchers found that breast cancer spreads when three specific cells are in direct contact: an endothelial cell (a type of cell that lines the blood vessels), a perivascular macrophage (a type of immune cell found near blood vessels), and a tumor cell that produces high levels of Mena, a protein that enhances a cancer cell's ability to spreaIn earlier studies involving animal models and human cancer cell lines, researchers found that breast cancer spreads when three specific cells are in direct contact: an endothelial cell (a type of cell that lines the blood vessels), a perivascular macrophage (a type of immune cell found near blood vessels), and a tumor cell that produces high levels of Mena, a protein that enhances a cancer cell's ability to spreain direct contact: an endothelial cell (a type of cell that lines the blood vessels), a perivascular macrophage (a type of immune cell found near blood vessels), and a tumor cell that produces high levels of Mena, a protein that enhances a cancer cell's ability to spread.

The researchers showed that cabazitaxel worked better than docetaxel in human prostate cancer cells lines that were resistant hormone treatment, both in terms of slowing cancer - cell growth and in its ability to kill cancer cells.

In addition to the afatinib - resistant NSCLC cells, the researchers tested the neratinib and valproic acid combination on cell lines derived from human pancreatic and ovarian cancers containing K - Ras mutations and N - Ras mutations, respectively.

A team of researchers in Germany and Denmark led by Steven Johnsen, Professor at the University Medical Center Göttingen, Germany, used human prostate cancer cell lines and depleted them of the DNA - binding protein CHD1.

He is researching the functions and properties of human sulfotransferase (EST) enzymes in human breast cancer cell lines.

Slashing the amount of HSF1 in several lines of human cancer cells diminished growth and survival.

«We've seen this in six different types of human cancer cell lines in the lab,» Choudhury said.

In studies of laboratory - grown human tumor cell lines, the drug disrupted tumor cell division and prevented growth of advanced cancer cells.

In preclinical experiments using human prostate cancer cell lines, Fu's team showed that increased PLK1 expression activated an oncogene known as c - RAF, which has previously been shown to play a role in regulating cell growth and divisioIn preclinical experiments using human prostate cancer cell lines, Fu's team showed that increased PLK1 expression activated an oncogene known as c - RAF, which has previously been shown to play a role in regulating cell growth and divisioin regulating cell growth and division.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in micIn the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in micin breast cancer - derived cell lines and in human breast cancers replicated in micin human breast cancers replicated in micin mice.

It demonstrates that alterations in these cells lines do indeed match changes in samples of human bladder cancer.

In a paper published online May 17, 2016 in the journal Nature Communications, de Jong and co-first author Koji Taniguchi, MD, PhD, and colleagues, report that treating both ERK1 / 2 and the compensatory pathway, ERK5, concurrently with a combination of drug inhibitors halted colorectal cancer growth more effectively in both mouse models and human colorectal cancer cell lineIn a paper published online May 17, 2016 in the journal Nature Communications, de Jong and co-first author Koji Taniguchi, MD, PhD, and colleagues, report that treating both ERK1 / 2 and the compensatory pathway, ERK5, concurrently with a combination of drug inhibitors halted colorectal cancer growth more effectively in both mouse models and human colorectal cancer cell linein the journal Nature Communications, de Jong and co-first author Koji Taniguchi, MD, PhD, and colleagues, report that treating both ERK1 / 2 and the compensatory pathway, ERK5, concurrently with a combination of drug inhibitors halted colorectal cancer growth more effectively in both mouse models and human colorectal cancer cell linein both mouse models and human colorectal cancer cell lines.

The goal of the first experiment was to see whether lncRNAs are differentially expressed in prostate cancer by measuring total RNA from prostate cancer cell lines and normal epithelial prostatic cells using NCode human ncRNA array and SurePrint G3 human lncRNA microarrays.

Dr. Bauman and her colleagues treated human head and neck cancer cells in the laboratory with varying doses of sulforaphane and a control, and compared them to normal, healthy cells that line the throat and mouth.

Recent studies with human breast cancer cell lines have also shown that Shp2 mediates survival signals in cancer cells.

For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.

To determine whether the reactivation of methylation - silenced genes by EGCG is a general phenomenon that also occurs in other cell lines, we studied the effects of EGCG treatment on the methylation status and mRNA levels of p16INK4a or RARβ in three other human cancer cell lines (Fig. 3C) ⇓.

The example shown reveals that these estrogen agonists show the strongest connectivity to each other in MCF7, a human breast cancer cell line that expresses the estrogen receptor.

Interestingly, two recent reports that have catalogued recurrent focal deletions in large cohorts of human tumors and cancer cell lines.

Sphere - forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines.

Decreased functional expression of Grp78 and Grp94 inhibits proliferation and attenuates apoptosis in a human gastric cancer cell line in vitro.

Significantly increased expression of OCT4 and ABCG2 in spheroid body - forming cells of the human gastric cancer MKN - 45 cell line.

The researchers then injected AAV2 into human breast cancer cell line - derived tumors in mice without functioning immune systems.

They fused mouse B cells — antibody - producing cells of the immune system — with human myeloma (also known as B cell cancer) cell lines in a new technique called hybridoma technology.

Using cutting - edge techniques enabled by next - generation sequencing, the authors generated complete methylome maps at single nucleotide resolution in a low - passage breast cancer cell line and normal breast tissue (primary human mammary epithelial cells).

Ab7291 staining alpha tubulin in human breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).

Pim - 3, a Proto - Oncogene with Serine / Threonine Kinase Activity, Is Aberrantly Expressed in Human Pancreatic Cancer and Phosphorylates Bad to Block Bad - Mediated Apoptosis in Human Pancreatic Cancer Cell Lines

«Basically, this study shows that the genetic makeup of individual human embryonic stem cell lines is unique in the numbers of copies of certain genes that may control traits and things like disease susceptibility,» said Teitell, who also is an associate professor of pathology and laboratory medicine and a researcher at UCLA's Jonsson Comprehensive Cancer Center.

I wanted to inactivate the gene BRAF (a kinase implicated in several human cancers) in A549 cells (a human lung cancer cell line), armed only with viruses obtained through Addgene's viral service and the methods sections of scientific articles (gasp).

The goal was to deepen the knowledge about the pharmacological and cytotoxic effects of the drug Temodex in vitro in different human brain cancer cell lines.

(a kinase implicated in several human cancers) in A549 cells (a human lung cancer cell line), armed only with viruses obtained through Addgene's viral service and the methods sections of scientific articles (gasp).

The human breast cancer cell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotcell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotCell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotics.

In a series of lab experiments with cell lines, human xenograft tumors in mice and primary human prostate cancer samples, the researchers demonstrated that miR - 34a inhibits prostate cancer stem cells by suppressing CD4In a series of lab experiments with cell lines, human xenograft tumors in mice and primary human prostate cancer samples, the researchers demonstrated that miR - 34a inhibits prostate cancer stem cells by suppressing CD4in mice and primary human prostate cancer samples, the researchers demonstrated that miR - 34a inhibits prostate cancer stem cells by suppressing CD44.

In studies using human cancer cell lines, a new, never - described compound known as BMH - 21 destroyed this critical communication pathway.

Inhibition of growth, production of insulin - like growth factor - II (IGFII), and expression of IGF - II mRNA of human cancer cell lines by antagonistic analogs of growth hormone - releasing hormone in vitro.

canSAR contains information on the entire human proteome — the protein equivalent of the human genome - as well as linking to information on more than 1,000 cancer cells lines, used by researchers to study the effect of new drugs in the lab.

Cultures of the human triple negative breast cancer cell line, HCC1806, were maintained in RPMI 1640 medium supplemented with 10 % FBS.

On the basis of studies on the XMRV - producing human prostate cancer cell lines CWR22Rν1 and CWR - R1 and their progenitor tumour xenograft CRW22, they concluded that XMRV infections were caused by contamination during in vivo passaging in nude mice.

Growth hormone (GH) receptors in prostate cancer: gene expression in human tissues and cell lines and characterization, GH signaling and androgen receptor regulation in LNCaP cells.

Important features of XMRV biology include (1) tropism for a variety of cell lines, including prostate cancer DU145 and LNCaP cells [27], [43], [48], and human neural cell types [57], (2) adaptations that promote growth in prostate epithelium and human - derived prostate cancer cell lines including an androgen response element in the promoter region [58] and downregulation of APOBEC3G [59], and (3) cellular effects with potential oncogenic properties including increased tumor aggressiveness mediated by downregulation of p27 [60] and differential regulation of several microRNAs [61].