Glucose oscillations, more than constant high glucose, induce p53 activation and a metabolic memory
in human endothelial cells
The p73 isoforms were not detectable
in human endothelial cells in the central and peripheral regions (Figure 1).
In human endothelial cell monolayers in culture, NS1 from any of the four DENV serotypes triggered endothelial barrier permeability.
Not exact matches
In experiments conducted on human lung endothelial cells and in mice, the researchers showed that NS1 caused permeability of the endothelium, which lines the walls of blood and lymph vessel
In experiments conducted on
human lung
endothelial cells and
in mice, the researchers showed that NS1 caused permeability of the endothelium, which lines the walls of blood and lymph vessel
in mice, the researchers showed that NS1 caused permeability of the endothelium, which lines the walls of blood and lymph vessels.
In a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collage
In a previous related study published
in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collage
in the Journal of Materials Science: Materials
in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collage
in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce
human umbilical vein
endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to
endothelial cells cultured on bovine collagen.
Specifically, the study — reported online
in The Journal of Infectious Diseases — shows that E. coli K1 modulates the protein peroxisome proliferator - activated receptor - gamma (PPAR - γ) and glucose transporter - 1 (GLUT - 1) levels at the blood - brain barrier
in human brain microvascular
endothelial cells.
In earlier studies involving animal models and human cancer cell lines, researchers found that breast cancer spreads when three specific cells are in direct contact: an endothelial cell (a type of cell that lines the blood vessels), a perivascular macrophage (a type of immune cell found near blood vessels), and a tumor cell that produces high levels of Mena, a protein that enhances a cancer cell's ability to sprea
In earlier studies involving animal models and
human cancer
cell lines, researchers found that breast cancer spreads when three specific
cells are
in direct contact: an endothelial cell (a type of cell that lines the blood vessels), a perivascular macrophage (a type of immune cell found near blood vessels), and a tumor cell that produces high levels of Mena, a protein that enhances a cancer cell's ability to sprea
in direct contact: an
endothelial cell (a type of
cell that lines the blood vessels), a perivascular macrophage (a type of immune
cell found near blood vessels), and a tumor
cell that produces high levels of Mena, a protein that enhances a cancer
cell's ability to spread.
In another test, the researchers looked to see if chemical signals released from the endothelial cells would cause the media layer to relax and constrict, as they do in the human bod
In another test, the researchers looked to see if chemical signals released from the
endothelial cells would cause the media layer to relax and constrict, as they do
in the human bod
in the
human body.
The researchers used the power of gene sequencing and clever computational methods to uncover the «source code» for
human endothelial cells and learn how that code is disturbed
in human disease.
In the chamber, tubes about the thickness of a human hair were lined with endothelial cells as in natural blood vessel
In the chamber, tubes about the thickness of a
human hair were lined with
endothelial cells as
in natural blood vessel
in natural blood vessels.
Malinski's team has developed unique methods and systems of measurements using nanosensors, which are about 1,000 times smaller
in diameter than a
human hair, to track the impacts of Vitamin D3 on single
endothelial cells, a vital regulatory component of the cardiovascular system.
The epithelium's maturation into a villus intestinal epithelium with long finger - like extensions was helped along by co-culturing
human intestinal microvascular
endothelial cells on the opposite side of the shared matrix - coated porous membrane
in the «vascular» channel where they assembled a surrogate blood vessel with a hollow lumen through which feeding medium was flowed.
In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channe
In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device
in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channe
in which
cells from a
human intestinal
cell line originally isolated from a tumor were cultured
in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channe
in one of two parallel running channels, separated by a porous matrix - coated membrane from
human blood vessel - derived
endothelial cells in the adjacent channe
in the adjacent channel.
We also demonstrate that NS1 from DENV1, DENV2, DENV3, and DENV4 triggers
endothelial barrier dysfunction, causing increased permeability of
human endothelial cell monolayers
in vitro.
Related to this, the discovery of an increased
endothelial cell population
in the periphery of the
human cornea has prompted an investigation for evidence of the existence of stem - like
cells in the
endothelial periphery.
The present demonstration of T
cell - mediated apoptosis of allogeneic corneal
cells from CD4 KO mice is consistent with previous findings, which noted the presence of apoptotic keratocytes and corneal
endothelial cells in rejected corneal allografts
in humans and rats respectively (5, 32).
Blake DA, Yu H, Young DL, Caldwell DR. Matrix stimulates the proliferation of
human corneal
endothelial cells in culture.
In keeping with this, recent studies showed that flavanols, a subclass of flavonoids that is richly represented in natural cocoa beans, increase NO production by cultured human vascular endothelial cells (16) and improve endothelium - dependent vasorelaxation (NO - dependent) in finger (2) and brachial (3) arteries of healthy human
In keeping with this, recent studies showed that flavanols, a subclass of flavonoids that is richly represented
in natural cocoa beans, increase NO production by cultured human vascular endothelial cells (16) and improve endothelium - dependent vasorelaxation (NO - dependent) in finger (2) and brachial (3) arteries of healthy human
in natural cocoa beans, increase NO production by cultured
human vascular
endothelial cells (16) and improve endothelium - dependent vasorelaxation (NO - dependent)
in finger (2) and brachial (3) arteries of healthy human
in finger (2) and brachial (3) arteries of healthy
humans.
It would seem, therefore, that cellular division has at least one significant negative control component
in both rabbit and
human corneal
endothelial cells whose activation may be delayed.
When
human corneal
endothelial cells were stimulated with EGF following wounding, staining also appeared
in the periphery (not shown).
Increased
endothelial cell density
in the paracentral and peripheral regions of the
human cornea.
Paull AC, Whikehart DR. Expression of the p53 family of proteins
in central and peripheral
human corneal
endothelial cells.
The impaired ability of
human corneal
endothelial cells (HCECs) to divide, both
in vivo and
in culture, has been well documented
in the scientific literature for a number of years [1 - 6].
BrdU staining with alkaline phosphatase was occasionally observed
in the wounded area's
human corneal
endothelial cells after wounding.
Initial imaging analysis of fluorescently labelled
human iPSC - derived hepatic endoderm
cells, umbilical cord - derived
endothelial cells (HUVECs), and mesenchymal stem
cells (MSCs) co-cultured
in a solidified matrix gel to promote 3D growth found that the different
cells collectively and automatically «condensed» into a multicellular central unit.
Specific inhibition of AQP1 water channels
in human pulmonary microvascular
endothelial cells by small interfering RNAs.
HIF - 2α expression regulates sprout formation into 3D fibrin matrices
in prolonged hypoxia
in human microvascular
endothelial cells.
Organ - on - a-chip technology may
in part overcome this limitation, as exemplified by the «breathing» lung - on - a-chip that recapitulates the alveolar - capillary interface by co-culturing
human alveolar epithelial
cells and capillary
endothelial cells on opposite sides of a flexible, porous, ECM - coated membrane.
Review of «Antitumor Effects of CD40 Ligand - Expressing
Endothelial Progenitor
Cells Derived From
Human Induced Pluripotent Stem
Cells in a Metastatic Breast Cancer Model» from Stem
Cells TM by Stuart P. Atkinson.
The Role of MGAT5
in Human Umbilical Vein
Endothelial Cells Possible Relevance to the Pathological Mechanism of Preeclampsia.
Highly active antiretroviral therapy drug combination induces oxidative stress and mitochondrial dysfunction
in immortalized
human blood — brain barrier
endothelial cells.
Researchers from the laboratories of Hiroshi Y. Yoshikawa and Hideki Taniguchi had previously demonstrated the
in vitro formation of a 3D transplantable liver «organ bud» from
human induced pluripotent stem
cells (iPSCs) co-cultured with mesenchymal and
endothelial progenitors, and allows for the growth of a small vascularized and functional organ [1 - 3].
Anastellin, a fragment of the first type III repeat of fibronectin, inhibits extracellular signal - regulated kinase and causes G1 arrest
in human microvessel
endothelial cells.
The
in vitro activity and specificity of
human endothelial cell - specific promoters
in porcine
cells.
The team identified the connections between rs9349379 and EDN1 by deleting a region of DNA at the SNP
in human pluripotent stem
cells and then converting these immature
cells into
endothelial cells and vascular smooth muscle
cells.
Production of factor VIII by
human liver sinusoidal
endothelial cells transplanted
in immunodeficient uPA mice.
By culturing RBECs and
human endothelial cells under flow conditions, the researchers found that
cell -
cell junctions they formed accurately mimicked
endothelial barrier formation
in the brain.
Susan Amara, USA - «Regulation of transporter function and trafficking by amphetamines, Structure - function relationships
in excitatory amino acid transporters (EAATs), Modulation of dopamine transporters (DAT) by GPCRs, Genetics and functional analyses of
human trace amine receptors» Tom I. Bonner, USA (Past Core Member)- Genomics, G protein coupled receptors Michel Bouvier, Canada - Molecular Pharmacology of G protein - Coupled Receptors; Molecular mechanisms controlling the selectivity and efficacy of GPCR signalling Thomas Burris, USA - Nuclear Receptor Pharmacology and Drug Discovery William A. Catterall, USA (Past Core Member)- The Molecular Basis of Electrical Excitability Steven Charlton, UK - Molecular Pharmacology and Drug Discovery Moses Chao, USA - Mechanisms of Neurotophin Receptor Signaling Mark Coles, UK - Cellular differentiation,
human embryonic stem
cells, stromal
cells, haematopoietic stem
cells, organogenesis, lymphoid microenvironments, develomental immunology Steven L. Colletti, USA Graham L Collingridge, UK Philippe Delerive, France - Metabolic Research (diabetes, obesity, non-alcoholic fatty liver, cardio - vascular diseases, nuclear hormone receptor, GPCRs, kinases) Sir Colin T. Dollery, UK (Founder and Past Core Member) Richard M. Eglen, UK Stephen M. Foord, UK David Gloriam, Denmark - GPCRs, databases, computational drug design, orphan recetpors Gillian Gray, UK Debbie Hay, New Zealand - G protein - coupled receptors, peptide receptors, CGRP, Amylin, Adrenomedullin, Migraine, Diabetes / obesity Allyn C. Howlett, USA Franz Hofmann, Germany - Voltage dependent calcium channels and the positive inotropic effect of beta adrenergic stimulation; cardiovascular function of cGMP protein kinase Yu Huang, Hong Kong -
Endothelial and Metabolic Dysfunction, and Novel Biomarkers
in Diabetes, Hypertension, Dyslipidemia and Estrogen Deficiency, Endothelium - derived Contracting Factors
in the Regulation of Vascular Tone, Adipose Tissue Regulation of Vascular Function
in Obesity, Diabetes and Hypertension, Pharmacological Characterization of New Anti-diabetic and Anti-hypertensive Drugs, Hypotensive and antioxidant Actions of Biologically Active Components of Traditional Chinese Herbs and Natural Plants including Polypehnols and Ginsenosides Adriaan P. IJzerman, The Netherlands - G protein - coupled receptors; allosteric modulation; binding kinetics Michael F Jarvis, USA - Purines and Purinergic Receptors and Voltage-gated ion channel (sodium and calcium) pharmacology Pain mechanisms Research Reproducibility Bong - Kiun Kaang, Korea - G protein - coupled receptors; Glutamate receptors; Neuropsychiatric disorders Eamonn Kelly, Prof, UK - Molecular Pharmacology of G protein - coupled receptors,
in particular opioid receptors, regulation of GPCRs by kinasis and arrestins Terry Kenakin, USA - Drug receptor pharmacodynamics, receptor theory Janos Kiss, Hungary - Neurodegenerative disorders, Alzheimer's disease Stefan Knapp, Germany - Rational design of highly selective inhibitors (so call chemical probes) targeting protein kinases as well as protein interaction inhibitors of the bromodomain family Andrew Knight, UK Chris Langmead, Australia - Drug discovery, GPCRs, neuroscience and analytical pharmacology Vincent Laudet, France (Past Core Member)- Evolution of the Nuclear Receptor / Ligand couple Margaret R. MacLean, UK - Serotonin, endothelin, estrogen, microRNAs and pulmonary hyperten Neil Marrion, UK - Calcium - activated potassium channels, neuronal excitability Fiona Marshall, UK - GPCR molecular pharmacology, structure and drug discovery Alistair Mathie, UK - Ion channel structure, function and regulation, pain and the nervous system Ian McGrath, UK - Adrenoceptors; autonomic transmission; vascular pharmacology Graeme Milligan, UK - Structure, function and regulation of G protein - coupled receptors Richard Neubig, USA (Past Core Member)- G protein signaling; academic drug discovery Stefan Offermanns, Germany - G protein - coupled receptors, vascular / metabolic signaling Richard Olsen, USA - Structure and function of GABA - A receptors; mode of action of GABAergic drugs including general anesthetics and ethanol Jean - Philippe Pin, France (Past Core Member)- GPCR - mGLuR - GABAB - structure function relationship - pharmacology - biophysics Helgi Schiöth, Sweden David Searls, USA - Bioinformatics Graeme Semple, USA - GPCR Medicinal Chemistry Patrick M. Sexton, Australia - G protein - coupled receptors Roland Staal, USA - Microglia and neuroinflammation
in neuropathic pain and neurological disorders Bart Staels, France - Nuclear receptor signaling
in metabolic and cardiovascular diseases Katerina Tiligada, Greece - Immunopharmacology, histamine, histamine receptors, hypersensitivity, drug allergy, inflammation Georg Terstappen, Germany - Drug discovery for neurodegenerative diseases with a focus on AD Mary Vore, USA - Activity and regulation of expression and function of the ATP - binding cassette (ABC) transporters
Figure 4 shows the mean survival curves obtained with the MTT assay
in human umbilical vein
endothelial cells (HUVEC), under conditions of normal and low proliferation rates (20 % and 2 % of FBS).
Three recent experimental studies focused on low consumption / exposure.949596
In one study, 29 smokers each consumed a single cigarette, immediately after which they had a significant decrease in blood vessel output power and significant increase in blood vessel ageing level and remaining blood volume 25 minutes later, as markers of atherosclerosis.94 In another study, human coronary artery endothelial cells were exposed to the smoke equivalent to one cigarette, which led to activation of oxidant stress sensing transcription factor NFR2 and up - regulation of cytochrome p450, considered to have a role in the development of heart disease.95 These effects were not seen when heart cells were exposed to the vapour from one e - cigarette.95 A study exposed adult mice to low intensity tobacco smoke (two cigarettes) for one to two months and found adverse histopathological effects on brain cells.
In one study, 29 smokers each consumed a single cigarette, immediately after which they had a significant decrease
in blood vessel output power and significant increase in blood vessel ageing level and remaining blood volume 25 minutes later, as markers of atherosclerosis.94 In another study, human coronary artery endothelial cells were exposed to the smoke equivalent to one cigarette, which led to activation of oxidant stress sensing transcription factor NFR2 and up - regulation of cytochrome p450, considered to have a role in the development of heart disease.95 These effects were not seen when heart cells were exposed to the vapour from one e - cigarette.95 A study exposed adult mice to low intensity tobacco smoke (two cigarettes) for one to two months and found adverse histopathological effects on brain cells.
in blood vessel output power and significant increase
in blood vessel ageing level and remaining blood volume 25 minutes later, as markers of atherosclerosis.94 In another study, human coronary artery endothelial cells were exposed to the smoke equivalent to one cigarette, which led to activation of oxidant stress sensing transcription factor NFR2 and up - regulation of cytochrome p450, considered to have a role in the development of heart disease.95 These effects were not seen when heart cells were exposed to the vapour from one e - cigarette.95 A study exposed adult mice to low intensity tobacco smoke (two cigarettes) for one to two months and found adverse histopathological effects on brain cells.
in blood vessel ageing level and remaining blood volume 25 minutes later, as markers of atherosclerosis.94
In another study, human coronary artery endothelial cells were exposed to the smoke equivalent to one cigarette, which led to activation of oxidant stress sensing transcription factor NFR2 and up - regulation of cytochrome p450, considered to have a role in the development of heart disease.95 These effects were not seen when heart cells were exposed to the vapour from one e - cigarette.95 A study exposed adult mice to low intensity tobacco smoke (two cigarettes) for one to two months and found adverse histopathological effects on brain cells.
In another study,
human coronary artery
endothelial cells were exposed to the smoke equivalent to one cigarette, which led to activation of oxidant stress sensing transcription factor NFR2 and up - regulation of cytochrome p450, considered to have a role
in the development of heart disease.95 These effects were not seen when heart cells were exposed to the vapour from one e - cigarette.95 A study exposed adult mice to low intensity tobacco smoke (two cigarettes) for one to two months and found adverse histopathological effects on brain cells.
in the development of heart disease.95 These effects were not seen when heart
cells were exposed to the vapour from one e - cigarette.95 A study exposed adult mice to low intensity tobacco smoke (two cigarettes) for one to two months and found adverse histopathological effects on brain
cells.96
Human corneal
endothelial cells expressed both p53 and TAp63; expression was greater
in the central endothelium than
in the peripheral endothelium (Figure 2).
However, p53 expression has previously been detected
in the cytoplasm of normal
human corneal
endothelial cells [13], and these
cells have a limited potential for
cell division.
Interferon -LCB- alpha -RCB- but Not Interleukin 12 Activates STAT4 Signaling
in Human Vascular
Endothelial Cells
Human endothelial cells exposed to BMP sprouting
in fibrin matrix.
Human pulmonary artery
endothelial cells cultured with serum from homozygous carriers showed an increase
in MCP - 1 release
in carriers of the minor allele, with the difference eliminated upon addition of tocilizumab.
Expression of the p53 family of proteins
in central and peripheral
human corneal
endothelial cells
Purpose: To determine the protein and mRNA expression of p53, p63, and p73
in central and peripheral
human corneal
endothelial cells.
This data should be useful for determining the growth potential and characteristics of
human corneal
endothelial cells in the development of artificial corneas.
The study was
in vitro, and not even on
human cells: they used bovine aortic
endothelial cells (BAECs).
In human beings with analogous conditions, a full - thickness or
endothelial corneal transplant replaces the lost
endothelial cells.