Some of these dyes, like Red No. 3, have been found to cause damage to the DNA
in human liver cells and to disrupt the function of the thyroid.
«To then determine how disrupting the gene might play out in the context of disease, we chemically knocked out the activity of SLC16A11
in human liver cells,» explains co-first author Victor Rusu, a former Harvard graduate student at Broad now at Jnana Therapeutics.
, to map the interaction between the proteins in HCV and
those in the human liver cells that HCV infects.
In a paper published in the journal Proceedings of the National Academy of Sciences, Sangeeta Bhatia of MIT and Charles Rice of Rockefeller University describe using microfabricated cell cultures to sustain hepatitis B virus
in human liver cells, allowing them to study immune responses and drug treatments.
There were also slight variations between how Zika establishes
itself in human liver cells versus neural stem cells, where it is more physiologically relevant.
Not exact matches
The researchers experimented with inducing oxidative stress
in a
human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme
in protecting the
cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the
liver.
Stem
cells have also been identified
in human milk, and have the potential to differentiate into mammary epithelial lineages under mammary differentiation conditions
in vitro, as well as other
cell types
in corresponding microenvironments, including bone
cells, brain
cells,
liver cells, pancreatic beta
cells and heart
cells.
In November 2010 Japanese researchers announced online in Analytical Chemistry that they had built a chip that simultaneously tests how liver, intestine and breast cancer cells respond to cancer drugs, and in February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cell
In November 2010 Japanese researchers announced online
in Analytical Chemistry that they had built a chip that simultaneously tests how liver, intestine and breast cancer cells respond to cancer drugs, and in February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cell
in Analytical Chemistry that they had built a chip that simultaneously tests how
liver, intestine and breast cancer
cells respond to cancer drugs, and
in February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cell
in February 2010 scientists publishing
in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cell
in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the
human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe
in cultured cell
in cultured
cells.
Liver cells carry out hundreds of different functions, only some of which Lagasse has tested
in mice, and it is unlikely that transplanted
cells could fulfill all of them
in humans.
Using a mathematical model known as the Ising model, invented to describe phase transitions
in statistical physics, such as how a substance changes from liquid to gas, the Johns Hopkins researchers calculated the probability distribution of methylation along the genome
in several different
human cell types, including normal and cancerous colon, lung and
liver cells, as well as brain, skin, blood and embryonic stem
cells.
If the procedure works
in humans, it would enable donated
livers from
humans, and possibly even from pigs, to be re-coated with a patient's own
cells, reducing the likelihood of organ rejection.
In this study, the Hiroshima University researchers developed an animal model using severely immunodeficient mice whose livers were partially populated with human cells, in order to reconstruct elements of the human immune syste
In this study, the Hiroshima University researchers developed an animal model using severely immunodeficient mice whose
livers were partially populated with
human cells,
in order to reconstruct elements of the human immune syste
in order to reconstruct elements of the
human immune system.
«We are the first to engineer a whole
liver organ with
human cells,» says Shay Soker, a co-developer of the
livers at Wake Forest University Baptist Medical Center
in North Carolina.
«Injecting the
human blood
cells resulted
in massive
liver cell damage and we were able to detect cytotoxic T lymphocytes that specifically targeted hepatitis B virus
in liver infiltration
cells.
Additionally, work
in a mouse model revealed similar
cells, indicating that the progenitors are conserved from mouse to
human, and therefore, they must be «important
cells with promising potential for
cell therapy
in treating
liver disease,» explained Dr. Gouon - Evans.
Next, the research team will examine specifically whether these
liver cells obtained from
human embryonic stem
cells in a dish help repair injured
livers in preclinical animal models of
liver disease.
This year they succeeded
in generating mini-livers, or
liver buds, from stem
cells that were taken from
human skin and reprogrammed to an embryonic state.
Neil Shay, a biochemist and molecular biologist
in OSU's College of Agricultural Sciences, was part of a study team that exposed
human liver and fat
cells grown
in the lab to extracts of four natural chemicals found
in Muscadine grapes, a dark - red variety native to the southeastern United States.
In recent years, several groups of scientists have grown lung
cells from
human iPSCs, but the recipes aren't perfect — the resulting lung
cells grow amidst a jumble of
liver cells, intestinal
cells, and other tissues.
To validate their computer modeling predictions, researchers performed experiments
in human cancer
cell lines, mouse
liver samples and primary
human hepatocytes.
Even more encouraging, the engineered tissues still continued to produce
human neural, cartilage, and
liver cell proteins, the team reports online this week
in the Proceedings of the National Academy of Sciences.
Scientists pre-treated
human liver cells in vitro with SBEL1 prior to HCV infection and found that SBEL1 pre-treated
cells contained 23 percent less HCV protein than the control, suggesting that SBEL1 blocks virus entry.
Rather than artificially triggering cancer by engineering genetic mutations, this model more closely mimics
human liver cancer
in that tumors develop as a natural consequence of non-alcoholic steatohepatitis (NASH), a chronic metabolic disorder that causes
liver damage, fibrosis and numerous
cell mutations.
The Hippo signaling pathway, which is highly conserved up to
humans, was known to play a critical role
in organ size determination, like, for example,
in the
liver, but has not been demonstrated to influence neural stem
cells in the central nervous system.
However, chronic
liver inflammation
in both mice and
humans also led to the accumulation of immunosuppressive lymphocytes, a type of immune
cell Karin and Shalapour first described two years ago.
Instead,
human ABCB4 has a specific function
in liver where it channels certain fatty acids into the bile ducts
in order to protect the
liver cells against aggressive biliary acids.
In research published in Molecular Cell, Rutgers scientists discovered that a protein (p62), which is supposed to act as an antioxidant to prevent cell damage, was not working efficiently in laboratory mice with liver and heart disease that mimicked these conditions in human
In research published
in Molecular Cell, Rutgers scientists discovered that a protein (p62), which is supposed to act as an antioxidant to prevent cell damage, was not working efficiently in laboratory mice with liver and heart disease that mimicked these conditions in human
in Molecular
Cell, Rutgers scientists discovered that a protein (p62), which is supposed to act as an antioxidant to prevent cell damage, was not working efficiently in laboratory mice with liver and heart disease that mimicked these conditions in hum
Cell, Rutgers scientists discovered that a protein (p62), which is supposed to act as an antioxidant to prevent
cell damage, was not working efficiently in laboratory mice with liver and heart disease that mimicked these conditions in hum
cell damage, was not working efficiently
in laboratory mice with liver and heart disease that mimicked these conditions in human
in laboratory mice with
liver and heart disease that mimicked these conditions
in human
in humans.
The Simon lab is now working on testing the effects of the chimera on
human liver cells and
in mouse
livers, to further elucidate its role
in the disease.
For the animal experiments, Savio Woo of the Center for Gene Therapy at Baylor College of Medicine
in Houston and his colleagues first isolated
liver cells from transgenic mice that produce the
human protein a1 - antitrypsin
in their
livers, from where it is secreted into the blood.
«We were excited to see that
in human liver tumors mTORC1 signaling correlates with FGF21 expression,» comments
cell biologist Dr. Marion Cornu and first author of the study.
TOKYO — A Japanese group has generated functional
human livers by creating
liver precursor
cells in the laboratory and then transplanting them into mice to complete the developmental process.
A cocktail of
human cell types mixed
in a dish (inset, left) spontaneously forms a three dimensional
liver bud (inset, right) which is transplanted into a mouse for final development into a
A ONE - OFF treatment for diabetes is a step closer thanks to a better understanding of how
human liver cells can be transformed into something like the beta
cells that produce insulin
in a healthy pancreas.
Writing
in the latest issue of the journal Nature, researchers
in the laboratories of Gladstone Senior Investigator Sheng Ding, PhD, and UCSF Associate Professor Holger Willenbring, MD, PhD, reveal a new cellular reprogramming method that transforms
human skin
cells into
liver cells that are virtually indistinguishable from the
cells that make up native
liver tissue.
Geneticist Yoav Gilad of the University of Chicago and his colleagues used a new technique to examine the genes
in the
liver cells of four primates:
humans, chimpanzees, orangutans and macaques.
Using
cells from mice and
human livers, Toronto General Hospital Research Institute researchers demonstrated for the first time how under specific conditions, such as obesity,
liver CD8 + T
cells, white blood
cells which play an important role
in the control of viral infections, become highly activated and inflammatory, reprogramming themselves into disease - driving
cells.
Now,
in a new study published
in the journal
Cell, scientists at the Salk Institute for Biological Studies have discovered that a synthetic form of vitamin D, calcipotriol (a drug already approved by the FDA for the treatment of psoriasis), deactivates the switch governing the fibrotic response
in mouse
liver cells, suggesting a potential new therapy for fibrotic diseases
in humans.
In further investigations of
human liver cells from nearly 50 donor tissues of
humans with varying degrees of body mass index (BMI) and
liver fat, higher levels of CD8 + T
cells were linked with higher levels of blood sugar or more advanced fatty
liver disease.
Salk researchers found that a drug already approved by the FDA for the treatment of psoriasis deactivates the switch governing the fibrotic response
in mouse
liver cells, suggesting a potential new therapy for fibrotic diseases
in humans.
The new cellular and molecular data uncovered
in the current study will be «exploited
in the future to further improve
liver bud organoids» and «precisely recapitulate differentiation of all
cell types»
in fetal
human development, the authors write.
Additionally, overexpression of POSTN
in human mammary epithelial and breast cancer
cells resulted
in enhanced tumor growth and metastasis (Wang et al., 2013), which is similar to a colon cancer
cell model where overexpression of POSTN resulted
in an increase
in the number and size of
liver metastases (Bao et al., 2004).
Researchers observed that the lab - grown
liver buds have molecular and genetic signature profiles that very closely resemble those found
in naturally developing
human liver cells.
The scientists took the genes for the most effective
liver cancer antigen receptors on those T
cells, put those receptors on
human T
cells and the resulting engineered
human T
cells eradicated the cancer as well, without hurting normal
liver cells, they report
in the journal Hepatology.
Next steps include He's collaboration with Piedmont Atlanta Hospital to retrieve T
cells,
liver cancer
cells and healthy tissue normally removed from patients during surgery, put the mouse receptor genes on these T
cells and monitor
in a dish both how those
cells now fight the tumor and react to healthy
human tissue.
Because of this, a major goal
in regenerative medicine is to attain self - organizing
human tissues —
in which
cells experience a series of coordinated molecular events precisely timed and spaced to form functioning three dimensional
liver buds, the authors write.
However, Takebe's
liver bud has the advantage of being grown from iPS
cells, rather than, for example, the primary
human hepatocytes used
in Bhatia's graft, which could make it useful
in modelling rare diseases or examining the specific genetic backgrounds of the iPS
cell donors.
If the marriage of stem
cells and CRISPR follows a similar path, it might not be long before pigs have enough Homo sapiens
in them not only to grow
human hearts, lungs,
livers, and kidneys for transplant but also to model
human diseases more closely than current lab animals do and to test experimental drugs.
«This data allows classification of all
human protein - coding genes into those coding for house - hold functions (present
in all
cells) and those that are tissue - specific genes with highly specialized expression
in particular organs and tissues, such as kidney,
liver, brain, heart, pancreas.
Human iPS
cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using our novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction
in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.
Liver X receptors and oxysterols promote ventral midbrain neurogenesis
in vivo and
in human embryonic stem
cells