Sentences with phrase «in lentiviral»

Genes associated with melanoma are overexpressed or silenced with shRNA constructs in lentiviral vectors and the lab increasingly uses cDNA and sh (short hairpin) RNA libraries for our experiments.

The vast majority of the immune cells, including 100 percent of the T and natural killer cells, carried the normal gene that was carried in the lentiviral vector.

One of the earliest steps in lentiviral transduction is the interaction between the viral envelop and the receptors on the target cell surface.

«The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events.»

The plasmids expressing hsa - mir -7-1 and hsa - mir -7-2 precursors in lentiviral pCDH - CMV - MCS - EF1 - copGFP vector (System Bioscience) were supplied by Yin - Yuan Mo (University of Mississippi Medical Center).

Not exact matches

The study also examines the use of lentiviral vectors for cell delivery in treating liver diseases, a tool traditionally used in treating blood disorders.

Single - cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter.

The treatment involves collected blood stem cells carrying the genetic anomaly of patients and corrected them in the laboratory by introducing a healthy WAS gene using a lentiviral vector developed and produced by Genethon.

OMC also aims at potentiating the existing Conditional Mutagenesis Facility by implementing novel approaches to attain tissue -, stage - and site - specific transgene expression in mouse mutant strains, mainly through lentiviral vector - mediated transduction of blastocysts.

However, their initial finding demonstrated that progenitor derived from iPSCs generated using lentiviral gene transduction led to the high incidence of highly aggressive tumors in immunodeficient mice after transplantation under the kidney capsule.

Lombardo A, Genovese P, Beausejour CM, Colleoni S, Lee YL, Kim KA, Ando D, Urnov FD, Galli C, Gregory PD, Holmes MC, Naldini L. Gene editing in human stem cells using zinc finger nucleases and integrase - defective lentiviral vector delivery.

Role of the endocytic pathway in the counteraction of BST - 2 by human lentiviral pathogens.

In addition to benefitting your cells, medium exchanges may also impact the quality of the lentiviral particles you harvest.

A method to assess target gene involvement in angiogenesis in vitro and in vivo using lentiviral vectors expressing shRNA.

Overgrown cultures stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as well as actively dividing cells resulting in lower lentiviral titers.

Functional KL2 genes were delivered to the mice using lentiviral vectors, based on a crippled version of HIV that was first developed by Verma and Salk Institute colleagues in 1996.

Lentiviral vectors are promising agents for cancer immunotherapy, but this research is still in the pre - clinical / early clinical stages.

lentiviral vectors contain a deletion in the 3» LTR that prevents aberrant activation of nearby genes.

Many CRISPR plasmids are designed for lentiviral use, including Addgene's top requested plasmid in 2015:

Lentiviral vectors are incredibly popular in the lab - the Trono lab's lentiviral packaging and envelope plasmids are two of Addgene's most requestedLentiviral vectors are incredibly popular in the lab - the Trono lab's lentiviral packaging and envelope plasmids are two of Addgene's most requestedlentiviral packaging and envelope plasmids are two of Addgene's most requested plasmids!

These images show the development of cancer (dark purple) in the mouse lung, initiated by lentiviral vector.

In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector.

Review of «Safety and efficacy of a tCD25 pre-selective combination anti-HIV lentiviral vector in human hematopoietic stem and progenitor cells» from Stem Cells by Stuart P. Atkinson

Their goal was to figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries.

Lentiviral vector technology has come a long way since the 1990s, both in terms of research impact and safety.

Lentiviral barcoding libraries have been used for complex applications like lineage tracing in heterogeneous tumors.

A lentiviral vector with the same modifier, Fuw - dCas9 - Tet1CD, is available from Rudolf Jaenisch's lab in plasmid form or as ready - to - use lentivirus.

In 2003, VIRxSYS began the first clinical trial with a lentiviral vector.

Suppression of clonal dominance in cultured human lymphoid cells by addition of the cHS4 insulator to a lentiviral vector.

Clinical benefits of lentiviral vectors have been observed in small trials for other diseases, including the common hematopoietic disorders

Although adeno - associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more genetic cargo (8 kb vs 4.5 kb), and they remain popular for targeting and tracing cells in the brain (Figure 1).

Lentiviral particles with the VSV - G envelope have high infectivity in a wide range of cell types, so they're ideal for work with primary cells or other difficult - to - transfect cells.

Since lentiviral vectors can deliver a large amount of DNA (~ 8 kb) with a relatively low immune response, it should come as no surprise that researchers are interested in developing these vectors for gene therapy.

To sidestep the shortcomings of currently used cancer models, the Salk team harnessed the power of lentiviral vectors to infect nondividing as well as dividing cells and ferry activated oncogenes into a small number of cells in adult, fully immunocompetent mice.

Lentiviral vectors in cancer immunotherapy.

Many CRISPR plasmids are designed for lentiviral use, including Addgene's top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome - wide screening.

The Morris lab is also active in developing cell targeted non-coding RNA delivery vehicles, such as lentiviral derived conditionally replicating vector systems, cell - targeted aptamers, and peptide conjugated RNAs.

Therapeutic levels of fetal hemoglobin in erythroid progeny of -LCB- beta -RCB-- thalassemic CD34 + cells following lentiviral vector - mediated gene transfer.

Extended ß - globin locus control region elements promote consistent therapeutic expression of a γ - globin lentiviral vector in murine ß - thalassemia.

1833 cells were incubated 48 hours with retroviral or lentiviral medium in the presence of Polybrene (8 μg / ml).

HEK293T cells were transfected with the following retroviral or lentiviral constructs, with their packaging vectors indicated in the parentheses, using FuGENE 6 reagent (Promega): pMX - puro - STAT5A * (provided by T. Kitamura, University of Tokyo; pCMV - Gag - Pol and pCMV - VSV - G); pLenti - EF - FH - TAZ - S89A (psPAX2 and pVSV - G); PLKO - NS and shTAZ lentiviral construct (provided by C. Linardic, Duke University; pCMV - Rev, pCVM - VSVG; pMDL).

Prior to transplantation, we analyzed the in vitro production of specific growth factors by unmodified hNPCctx and by hNPCctx transduced with a lentiviral gene construct designed to constitutively express GDNF (hNPCctx - GDNF)[11], [44].

In this post, I'll summarize the basic steps and analyses, and give what I think are the main tips for each step of performing and analyzing a gene edit using Addgene's lentiviral CRISPR tools.

Large - Scale Production of Lentiviral Vector in a Closed System Hollow Fiber Bioreactor.

The defect in proplatelet formation was also observed in control CD34 (+) cell - derived megakaryocytes transduced with lentiviral particles encoding mutant ETV6.

We also demonstrated that this observation was not caused incidentally by a particular vectoring system: using intraductal injection of a lentiviral vector (Bu et al., 2009) to introduce caErbb2 to ductal epithelia, we also observed accelerated tumorigenesis in the parous group relative to the virgin group (Figure 1 — figure supplement 2).