Sentences with phrase «in microarray analysis»

Yet, pericytes themselves have been shown to be a primary source of Ang1 (refs 21, 22, 23)(which was confirmed in the microarray analysis (Fig. 1a)-RRB-, suggesting an autocrine mode of action.

Apart from science and engineering, bioinformatics is a vital tool in microarray analysis due to the vast amounts of data that are generated from a microarray experiment.

Not exact matches

Other labs have used DNA microarray analysis to characterize gene expression changes in this model.

Headquartered in San Diego, California, Illumina develops microarray technologies used in large - scale analysis of genetic variation and function.

Jason: I was analyzing some microarray data, and I reached the limit of what I knew how to do, in terms of data analysis.

Typical microarray - based gene expression analyses compare gene expression in adjacent normal and cancerous tissues.

They have now used the tools of Social Network Analysis (SNA) to help them unravel the connections and identify the biomarkers present in patient genomic microarray data.

Stephen W. Scherer, Ph.D., of the Hospital for Sick Children, Toronto, and colleagues performed chromosomal microarray analysis (CMA) and whole - exome sequencing (WES) in a group of 258 unrelated children with ASD to determine the molecular diagnostic yield (the percentage of subjects with a genetic alteration [mutation] that may contribute to the features of autism spectrum disorder) of these tests.

A: Sheltzer: I was analyzing some microarray data, and I reached the limit of what I knew how to do in terms of data analysis.

For expression analysis in mice, we used microarray data as described above to select two internal control genes, cyclophilin B (Cphn2) and ribosomal protein S3 (Rps3).

GeneTraffic is a bioinformatics server system for microarray data storage and analysis in Minimum Information about a Microarray Experiment (MIAME) compliant fomicroarray data storage and analysis in Minimum Information about a Microarray Experiment (MIAME) compliant foMicroarray Experiment (MIAME) compliant format [50].

The analysis of doxorubicin resistance in human breast cancer cells using antibody microarrays.

SAM analysis was used to identify statistically significant changes in microarray data, and the bioinformatics programs GoMiner, Gene Set Enrichment Analysis (GSEA), and HiMAP were used to identify significant ontological categories and analyze the N - methyl - D - aspartate (NMDA) receptor inteanalysis was used to identify statistically significant changes in microarray data, and the bioinformatics programs GoMiner, Gene Set Enrichment Analysis (GSEA), and HiMAP were used to identify significant ontological categories and analyze the N - methyl - D - aspartate (NMDA) receptor inteAnalysis (GSEA), and HiMAP were used to identify significant ontological categories and analyze the N - methyl - D - aspartate (NMDA) receptor interactome.

Gene Set Enrichment Analysis (GSEA) is a microarray data - mining technique used to determine whether there is coordinated differential expression or «enrichment» in a set of functionally related genes when comparing control and experimental samples [35].

The department has expertise and infrastructure to support bacterial and viral analysis, and the detailed evaluation of cellular and serological immune responses including flow cytometry and cell sorting, Luminex multiplex platforms, real time PCR, microarrays, comprehensive histological staining, immunohistochemistry and in - situ hybridisation.

The Sequencing and Microarray Facility provides essential genomic analysis services to support researchers in basic science, translational, and populationbased research programs

This will be achieved by exposing the coral to seawater with high calcium concentrations to induce an alteration in gene expression profiles, identified using a DNA microarray analysis.

Parallel whole - genome microarray analysis was used to determine the transcriptome in matched samples.

Along with the cellular genes emphasized from the microarray analysis, two genes that are suspected to be involved in the scleractinian coral calcification process were tested for expression (Figs. 5A and 5B).

Clinical Diagnostics The platform will provide service and support in order to promote and adapt the use of high throughput techniques, such as next - generation sequencing, microarray - based analyses and multiplex protein analysis, in routine diagnostics.

In addition, analysis of the microarray results obtained with RNA from the optimized protocol showed that, as expected, the genes involved in methane production (methanogenesis) were among the most highly expressed genes in M. barkerIn addition, analysis of the microarray results obtained with RNA from the optimized protocol showed that, as expected, the genes involved in methane production (methanogenesis) were among the most highly expressed genes in M. barkerin methane production (methanogenesis) were among the most highly expressed genes in M. barkerin M. barkeri.

Microarray analysis revealed temperature - dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype.

At Berkeley, she developed computationally intensive statistical methods for the analysis of microarray data with applications in cancer biology.

Proteomic analysis to profile protein abundance resulted in the identification and relative quantification for 912 proteins with two or more unique peptides and 86 proteins with significant abundance changes after treatment with the neurotoxins, while microarray analyses to profile gene expression revealed 181 genes with significant changes in mRNA after treatment.

Demonstration of loss of heterozygosity by single - nucleotide polymorphism microarray analysis and alterations in strain morphology in Candida albicans strains during infection.

Research on vaccine biomarkers, including in - depth comparative analysis of data from different platforms, large - scale RNA sequencing, harmonisation of standard operating procedures (SOPs) for sample, microarray and data analysis, as well as transcriptome mapping (more than 1400 samples analysed).

Finally, the researchers used microarray analysis to investigate the gene expression and molecular pathways in the muscles of DS mice.

We also performed microarray analysis of PyMT - Slc6a14 + / + and PyMT - Slc6a14 − / − mammary tumours to evaluate the expression of amino acid transporters without any biased preconception of the identity of the transporters that might be subject to changes in expression in association with Slc6a14 deletion.

Microarray analysis of prostate cancer progression to reduced androgen dependence: studies in unique models contrasts early and late molecular events.

Similarly, microarray analyses comparing global gene expression in cells that overexpress wild - type STAT3 or phosphorylation - deficient mutant indicate that a subset of genes are regulated by unphosphorylated STAT3, including well - known oncogenes such as MRAS and MET (Yang et al., 2005).

ViroChip microarray, PCR, fluorescence in situ hybridization (FISH), serological, and deep sequencing analyses were performed in 4 different laboratories (Fig. 1).

Waters, who is director of biological sciences at PNNL, was invited to be on the committee because of her strong expertise in microarray and omics data analysis.

Finally, RNA samples were prepared following the same procedure and subsequent microarray analyses were performed in a central facility using Affymetrix Mouse 430 v. 2 arrays.

Microarray analysis, the researchers found, revealed genetic conditions in 2.5 % of the pregnancies with a normal karyotype.

Microarray Analysis of Retinal Gene Expression in the DBA / 2J Model of Glaucoma Steele, MR., Inman DM., Calkins, D.J., Horner, P.J. & Vetter, M.L. (2006) Investigative Ophthalmology & Visual Science, 47:977 - 985.

264/5: 45 Diagnostic utility of whole genome sequencing as an alternative to chromosomal microarray analysis in pediatric medicine.

Normalisation and statistical analysis of microarray data was performed in R using the Bioconductor packages Limma, Affy and BeadExplorer.

In brief, 330 of 364 genes, tested by quantitative or conventional PCR, gave comparable expression patterns to the data obtained by microarray analysis.

As part of an ongoing functional genomic screening approach, we have been using microarray analysis and next generation sequencing to identify mouse genes that are specifically expressed in meiosis, and are likely involved in aspects of chromosome biology that are required for meiotic ploidy reduction.

«But a major limitation of cluster analysis,» he said, «is that it doesn't use information external to the microarray data - the kinds of things that are important in solving problems of biological interest.»

Microarray analysis yielded results in 87 % of the stillbirths, compared to 70 % for karyotype analysis.

Prior to working at City of Hope, she worked as a research associate in Dr. Peter Lee's lab at Stanford University since 2006 and facilitated in investigating mechanisms causing immune dysfunction in melanoma and breast cancer patients» peripheral blood, lymph node and tumor specimens utilizing techniques including microarray analysis, RT - PCR, Phospho - flow cytometry, histoloogy, and functional FACS studies.

In addition to analysis using the full HuProt microarray, CDI can custom print microarrays containing up to 14 separate subarrays - each containing only proteins of interest as determined during the discovery phase.

The immunopurification (IP) of Argonaute (Ago), a central component of the RISC in the human and mouse, followed by microarray analyses (Ago IP / microarray method) makes it possible to isolate any Ago - associated miRNAs and mRNAs without relying on the mechanism of regulation (i.e. mRNA decay or translational suppression), or sequence conservation, enabling a comprehensive identification of the miRNA - target genes in an unbiased manner.

Combining our in vitro assay with microarrays analyses, we have uncovered previously unknown effectors and target genes of Wnt.

«Low - Calorie Diet Slows Aging in Mice in Study,» claimed a recent headline.17 According to the article, «Putting elderly mice on a very low - calorie diet for as little as four weeks reversed many of the changes in the activity of various genes that had occurred during normal aging...» The resesearchers were not looking at actual signs of disease, nor were they measuring lifespan, but instead focused on the analysis of 11,000 different genes using a method called microarray technology in which Spindler has large financial holdings.

Additionally, in March 2009, NASA awarded CombiMatrix $ 858,000 from NASA over three years for the Company's semiconductor microarray that can be used in automated genetic analyses suitable for use in satellites.

Experienced in developing pipelines and subsequent analysis / interpretation of high - throughput array data (microarrays, SNPs and CNVs), NGS data (RNA - Seq, miRNA - Seq and ChIP -...

A detailed description of the methods and analyses of methylated DNA immunoprecipitation (MeDIP) and microarrays hybridization used in this study were previously described [43] and can be found in File S1.

Q - MeDIP analysis of DNA methylation differences between CPA (black) and control (white) groups in men and women T cells samples for four gene promoters predicted to be more methylated in the men CPA group by microarray analysis.