Sentences with phrase «in nucleosomes»

The activation induced cytidine deaminase (AID) efficiently targets DNA in nucleosomes, but only during transcription.

O'Sullivan came to the Karlseder lab seven years ago to explore whether histone / DNA interactions in nucleosomes altered telomere function.

The authors conclude that this newly discovered allosteric mechanism «suggests that allosteric modulation in nucleosomes may have biological relevance and potential for therapeutic interventions.»

Differentially methylated regions are hypomethylated and enriched in nucleosome - retaining regions.

In a study published in Molecular Cell this month, Alexei V. Tulin, PhD, Associate Professor at Fox Chase Cancer Center, and colleagues reported that chemical modification of one type of histone — called H2Av — leads to substantial changes in nucleosome shape.

In the new study, Tulin and his colleagues reevaluated how PARP1 is activated by changes in the nucleosome.

Gene ontology analysis of the genes affected across several tissues (Supplementary Fig. 8) shows enrichment for genes in the extracellular region and genes involved in nucleosome and chromatin assembly and in protein — DNA complexes.

Not exact matches

In this way the binding of the DNA to the corresponding nucleosome is changed so that the DNA for example becomes accessible for transcription enzymes and activates a particular gene.

In order to fit inside cells, DNA is wrapped around small protein spindles, forming strings of molecules called nucleosomes.

ASF1 in fact «chaperones» a histone protein, which when coiled in a complex with DNA, forms the basic structural building block of DNA called a nucleosome.

In January, a team at the University of Washington, Seattle, described in Cell a test that relies on tissue - specific differences in how DNA is packaged in structures called nucleosomeIn January, a team at the University of Washington, Seattle, described in Cell a test that relies on tissue - specific differences in how DNA is packaged in structures called nucleosomein Cell a test that relies on tissue - specific differences in how DNA is packaged in structures called nucleosomein how DNA is packaged in structures called nucleosomein structures called nucleosomes.

«Our cryo - EM images help us understand how the complex can recognize the presence of a histone modification in one nucleosome and place the same tag onto a neighboring nucleosome.»

Researchers now plan to test the nucleosome and methylation approaches in larger groups of people.

The helix winds onto nucleosomes to form chromatin, which winds and winds in its turn into formations similar to what you get when you keep twisting the two ends of a string.

In three out of five people with advanced cancers, the team could use those nucleosome fingerprints to trace circulating DNA to the cancerous tissue.

Put simply, nucleosomes and methylation can affect transcription leading to changes in cells.

The ability of PARP1 to control cellular processes is regulated by nucleosomes — the basic unit of DNA packaging, consisting of a segment of DNA wound in sequence around eight histone protein cores, similar to a thread wrapped around a spool.

In this system, the DNA strand, with its genes, is coiled around molecules known as histones, which themselves are assembled into larger entities called nucleosomes.

Since many of the genes containing «fragile» nucleosomes are controlled in a continuous manner by nutrient availability, modulation of promoter nucleosome stability may be a strategy used to coordinate growth - related transcription on a genome - wide level.

«The genome probably stays in a very compact state most of the time, with nucleosomes winding the DNA like a tight spring.

«It is important to understand how nucleosomes are moved, ejected or restructured, as this will affect the accessibility of promoter DNA, which in turn influences the expression of the corresponding genes,» explains David Shore, professor at the Department of Molecular Biology of the Faculty of Science at UNIGE.

They speculate that the dynamic nature of the «fragile» nucleosome plays an important role in increasing access of the proteins initiating the transcription to the promoter.

«It's the packing density that could change and limit the accessibility of chromatin, providing a local and global structural basis through which different combinations of DNA sequences, nucleosome variations and modifications could be integrated in the nucleus to exquisitely fine - tune the functional activity and accessibility of our genomes.»

The dynamics of nucleosome formation and positioning in promoters may, for example, help to understand why some genes are highly expressed, such as those involved in normal or malignant growth, while others, such as stress - induced genes, are only rarely expressed under normal conditions.

When a given gene needs to be transcribed to create new proteins, its promoter region must be unwrapped from the nucleosome so that it can be accessed by the factors involved in initiating the transcription process.

X-rays and microscopy showed that the primary level of chromatin organization involves 147 bases of DNA spooling around proteins to form particles approximately 11 nanometers (nm) in diameter called nucleosomes.

In collaboration with researchers at the Department of Computer Science (UNIGE) and the Swiss Institute of Bioinformatics at EPFL, David Shore's team has undertaken to characterize nucleosomes present in every gene promoter of yeast DNIn collaboration with researchers at the Department of Computer Science (UNIGE) and the Swiss Institute of Bioinformatics at EPFL, David Shore's team has undertaken to characterize nucleosomes present in every gene promoter of yeast DNin every gene promoter of yeast DNA.

One type, characterized by the presence of dynamic, unstable, nucleosomes, is found at highly expressed genes, such as those involved in the control of cell growth and division.

In 2012, for example, Willerslev's lab published an analysis of proteins, which are generally longer lived postmortem than genetic material, of 43,000 - year - old woolly mammoth bones.16 And last year, Willerslev, Orlando, and colleagues published a genome - wide nucleosome map and survey of cytosine methylation levels in the DNA they pulled from the 4,000 - year - old hair shafts of a Paleo - Eskimo, effectively launching the field of ancient epigenetics.17 Also last year, Pääbo's group at the Max Planck Institute for Evolutionary Anthropology published the first full DNA methylation maps of the Neanderthal and Denisovan genomes.18 «For the first time we'll be able to address what is the role of epigenomics and epigenetics in evolution,» Willerslev sayIn 2012, for example, Willerslev's lab published an analysis of proteins, which are generally longer lived postmortem than genetic material, of 43,000 - year - old woolly mammoth bones.16 And last year, Willerslev, Orlando, and colleagues published a genome - wide nucleosome map and survey of cytosine methylation levels in the DNA they pulled from the 4,000 - year - old hair shafts of a Paleo - Eskimo, effectively launching the field of ancient epigenetics.17 Also last year, Pääbo's group at the Max Planck Institute for Evolutionary Anthropology published the first full DNA methylation maps of the Neanderthal and Denisovan genomes.18 «For the first time we'll be able to address what is the role of epigenomics and epigenetics in evolution,» Willerslev sayin the DNA they pulled from the 4,000 - year - old hair shafts of a Paleo - Eskimo, effectively launching the field of ancient epigenetics.17 Also last year, Pääbo's group at the Max Planck Institute for Evolutionary Anthropology published the first full DNA methylation maps of the Neanderthal and Denisovan genomes.18 «For the first time we'll be able to address what is the role of epigenomics and epigenetics in evolution,» Willerslev sayin evolution,» Willerslev says.

In an interdisciplinary team effort, we study the conformation of the genome at various scales, from the nucleosome fiber to the distribution of chromosomes territories in the nuclear spacIn an interdisciplinary team effort, we study the conformation of the genome at various scales, from the nucleosome fiber to the distribution of chromosomes territories in the nuclear spacin the nuclear space.

There was a striking pattern of such motifs repeated about 10.5 bp downstream of the dominant TSS, suggesting that the nucleosomes have something to do with TSS preference in broad promoters.

Evidence of heterogeneous distribution of nucleosomes in chromatin fibers using STORM super-resolution imaging (Cosma's group, Cell, 2015).

Nucleosome maps of the human cytomegalovirus genome reveal a temporal switch in chromatin organization linked to a major IE protein.

Intriguingly, in contrast to the canonical nucleosome (where 147 bp of DNA are wrapped around the histone octamer), only the central 121 bp were visible, suggesting flexible CENP - A nucleosomal ends.

The data illustrates how RSC remodels the nucleosome in vitro and shed light on its in vivo function.

The DNase I data exploits a structural feature of regulatory DNA that's been known for decades: the binding of sequence - specific transcriptional regulators in place of nucleosomes creates DNase I hypersensitive sites, or DHSs.

Sequencing of DNase I hypersensitive sites in (125 cell types), FAIRE analysis of reduced nucleosome crosslinking (25 cell types), and genomic DNase I footprinting (41 cell types).

In a biochemical study in 2017 in eLife, Panne's group reported on the first step of nucleosome assemblIn a biochemical study in 2017 in eLife, Panne's group reported on the first step of nucleosome assemblin 2017 in eLife, Panne's group reported on the first step of nucleosome assemblin eLife, Panne's group reported on the first step of nucleosome assembly.

In the interferon beta system, the transcription factors, or «enhanceosome,» lands on a stretch of DNA between nucleosomes.

In contrast, closed chromatin (heterochromatin) is inaccessible because it's tightly wrapped around nucleosomes.

In a second study, published in 2016 in the EMBO Journal, the Panne group reported on the second nucleosome assembly steIn a second study, published in 2016 in the EMBO Journal, the Panne group reported on the second nucleosome assembly stein 2016 in the EMBO Journal, the Panne group reported on the second nucleosome assembly stein the EMBO Journal, the Panne group reported on the second nucleosome assembly step.

Similar affinity - tagged CENP - A nucleosomes assembled in vitro may also be used to purify components that selectively target to such nucleosomes.

Linking DNA methyltransferases to epigenetic marks and nucleosome structure genome - wide in human tumor cells.

BAZ2A is an essential component of the nucleolar remodeling complex (NoRC), which mediates recruitment of histone modifyine enzymes and DNA methylase involved in the silencing of ribosomal RNA transcription by RNA polymerase I. BAZ2B is believed to be involved in regulating nucleosome mobilization along linear DNA.

We have developed a quantitative nucleosome assembly (NAQ) assay that measures nucleosome formation in vitro (Figure 2).

The Cac2 subunit is essential for productive histone binding and nucleosome assembly in CAF - 1.

269/5: 00 Cell - free DNA comprises an in vivo nucleosome footprint that informs its tissue (s)- of - origin.

While somatic cells have dense and compacted clutches that contain tens of nucleosomes, clutches in pluripotent cells contain fewer and less compacted nucleosomes.

Correlation coefficients of total expression levels (log10) with nucleosome formation potential scores in housekeeping (Hkg) and tissue specific genes (Tsg).

Looking more closely at the passage above and comparing it to the content of the National Science Education Standards (NSES)(National Research Council, 1996) shows that a single paragraph from the most commonly used high school biology textbook in the United States includes at least six scientific terms (eukaryotic, chromosome, prokaryote, chromatin, histone, and nucleosome) that are, unlike DNA and protein, not included in the NSES.