Sentences with phrase «in oocyte maturation»

Both animal and human studies have demonstrated the important roles of neurotrophins (BDNF, NTF3 and NTF4) in oocyte maturation mediated by their receptors (NTRK1 — 3 and NGFRAP1)[20], [25].

To find out more about the pathway's role in oocyte maturation, Nick Duesbery, a postdoc in Vande Woude's lab, was seeking compounds that block MAPK's activity.

Not exact matches

They were interested in one of the cell's key signaling pathways, the MAPK pathway, which helps control cell growth, embryonic development, and the maturation of oocytes into eggs.

In biology, folliculogenesis refers to the maturation of the ovarian follicle, a densely - packed shell of somatic cells that contains an immature oocyte.

Following incubation in medium supplemented with ovarian factors (BDNF, IGF - I, estradiol, GDNF, FGF2 and leptin), a greater percentage of oocytes demonstrated nuclear maturation and subsequently, underwent parthenogenesis relative to control.

As outlined below, we used a microfluidic quantitative PCR (qPCR) system to elucidate the gene expression profiles of individual human oocytes and small numbers of cumulus cells using a combination of a large number of samples and targets [12], and then extended our studies via the use of parthenogenesis, in conjunction with gene expression profiling, as a functional assay of cytoplasmic maturation of oocytes.

Oocyte maturation and embryo development data were entered into a two - by - two contingency table, and Fisher's exact test was used to generate P - values in Prism version 5.02 for windows (GraphPad Software, Inc.).

However, detailed protein expression of individual ovarian factors and their receptors in oocytes need to be further studied in order to elucidate the factors that may affect oocytes in specific maturation stages.

In contrast, nuclear maturation of MI oocytes was much more efficient with values ranging from 75 to 94 % in the three different mediIn contrast, nuclear maturation of MI oocytes was much more efficient with values ranging from 75 to 94 % in the three different mediin the three different media.

We derived a protocol for paracrine / autocrine supplementation for in vitro maturation of cumulus - free oocytes that includes supplementation with BDNF, IGF - I, GDNF, leptin, FGF2 and estradiol as shown in Figure 2C.

In order to improve culture conditions for maturation of cumulus - free oocytes in vitro, we began by assaying the expression of 15 growth factors (BDNF, IGF - I, estradiol, GDNF, leptin, FGF1, FGF2, GM - CSF, EGF, TGF - α, TGF - β1; 2; 3, and ET - 1; 2) and 27 of their cognate receptors that have been shown to regulate oocyte maturatioIn order to improve culture conditions for maturation of cumulus - free oocytes in vitro, we began by assaying the expression of 15 growth factors (BDNF, IGF - I, estradiol, GDNF, leptin, FGF1, FGF2, GM - CSF, EGF, TGF - α, TGF - β1; 2; 3, and ET - 1; 2) and 27 of their cognate receptors that have been shown to regulate oocyte maturatioin vitro, we began by assaying the expression of 15 growth factors (BDNF, IGF - I, estradiol, GDNF, leptin, FGF1, FGF2, GM - CSF, EGF, TGF - α, TGF - β1; 2; 3, and ET - 1; 2) and 27 of their cognate receptors that have been shown to regulate oocyte maturation.

Following in vitro maturation, oocytes at metaphase II with the presence of a first polar body were activated using 10 µM calcium ionophore A23187 (Sigma - Aldrich) for 5 min followed by 2 mM DMAP (Sigma - Aldrich) for 4 h at 37 °C in 6 % CO2, 5 % O2 and 89 % N2.

Since the first report of in vitro human oocyte maturation in 1969 [3], several reports have documented blastocyst (BL) development or live birth achieved from oocytes matured in vitro [1], [4], [5].

Based on our gene expression data, FGF2 could affect oocyte maturation in a paracrine manner via the cumulus cells.

Oxygen concentration during mouse oocyte in vitro maturation affects embryo and fetal development.

Simulated physiological oocyte maturation (SPOM): a novel in vitro maturation system that substantially improves embryo yield and pregnancy outcomes.