Recently, the lab disabled 62 genes (
in a pig genome) all in one go — the largest number of simultaneous gene edits ever accomplished.
Not exact matches
As for «playing God» — the argument that it is unethical to change a
pig in the way that
genome - editing does — she retorts that «the highest moral standard is human life.
Working out how to do it
in the
pigs would point the way toward, say, adding copies of the cancer - fighting gene p53 into a person's
genome.
Many people feared that retroviruses lurking
in animal
genomes could harm transplant recipients, but this year a team eliminated,
in one fell swoop, 62 copies of a retrovirus's DNA littering the
pig genome.
Subsequent analysis of two - thirds of the
genome in the 2009 A (H1N1) isolated from more than a dozen
pigs shows that it closely matches the human version of the virus.
Eager to get the record straight, Laurent Frantz, now a bioinformaticist at the University of Oxford
in the United Kingdom, carried out sophisticated computer analyses of 103 whole
genomes sequenced from wild boars and domesticated
pig breeds from all over Europe and Asia.
Farmers may have domesticated
pigs 9000 years ago, but
genome studies now show that
in those early centuries, trysts with wild animals were quite common, particularly
in Europe.
His adviser at the time, animal genomicist Martien Groenen of Wageningen University and Research Centre
in the Netherlands, had sequenced these
genomes and had gathered additional, albeit less complete, genetic data from 600 other wild and domesticated
pigs as part of another study.
There are even a few islands — those that contain genes involved
in behavior and size, key traits for domesticated animals — that are
in the
genomes of both European and Asian
pigs, Larson reports.
This is one of the first population genomics analyses to use high - coverage whole -
genome sequencing
in pigs, each individual has been sequenced more than 20-fold depth with the
genome coverage of 95 %.
Substantially, a nearly complete catalogue of the genetic variants has been compiled, which allowed the scientist to identify a
genome - wide set of loci for local adaptation
in Chinese
pigs.
We could say that the Iberian
pig is very similar to the
pigs which existed
in the sixteenth century and no great changes have been registered
in this
genome.
A
genome - wide scan for signatures of directional selection
in domesticated
pigs.
Adaptation and possible ancient interspecies introgression
in pigs identified by whole -
genome sequencing Ai, H., X. Fang, B. Yang, Z. Huang, H. Chen et al. 2015.
Now, three decades after those weapons were destroyed, scientists have sequenced the
genome of the organism — a pathogen typically found
in pigs called Brucella suis.
The major project to chart the
pig genome shows that the wild boar originated
in Southeast Asia about 4 million years ago.
Recently, prospects for xenotransplantation brightened when Harvard geneticist George Church demonstrated the removal of dozens of endogenous retroviruses from the
pig genome,
in a tour de force of the CRISPR / Cas9 gene editing technique.
«The
pig is one of our most important domesticated animals, and it was high time for its
genome to be mapped», says Professor Leif Andersson, who participated
in the project.
Uppsala researchers Patric Jern, Alexander Hayward, Göran Sperber, and Jonas Blomberg used the computer program RetroTector and detailed sequence comparisons
in so - called phylogenetic studies to map the retrovirus part of the
pig genome.
The efficient
genome editing shown here demonstrates that these
pigs can serve as a powerful tool for dissecting
in vivo gene functions and biological processes
in a temporal manner and for streamlining the production of
genome - edited
pigs for disease modeling.
To further facilitate
genome editing
in pigs, we report here establishment of a
pig line with Cre - inducible Cas9 expression that allows a variety of ex vivo
genome editing
in fibroblast cells including single - and multigene modifications, chromosome rearrangements, and efficient
in vivo genetic modifications.