Sentences with phrase «in plasma samples»

Kristin Møller Gabrielsen of the Norwegian University of Science and Technology in Trondheim and colleagues report in the journal Environmental Research that they examined the liver, muscle and kidney tissues taken from seven polar bears killed by Inuit hunters in East Greenland in 2011 and analysed the effect of more than 50 contaminants in plasma samples from Ursus maritimus, to see what effect organohalogen compounds could have on the bears» thyroid systems.

(D — I) Blood samples were taken at the indicated times, and radioactivity remaining in the plasma samples (10 μl) was determined by liquid scintillation counting (n = 3 — 4 / group).

Notably, we studied the feasibility for enrichment of ctDNA by physical and in silico size selection in plasma samples collected in 51 patients from multiple cancer types.

The Joslin researchers used global mass spectrometry to look for levels of approximately 2,400 metabolites (molecules produced during metabolism) in plasma samples from the patients.

«Based on our findings, the next phase will be to measure exosomes and exomeres in plasma samples to help predict organs that may be targeted for metastasis during tumor progression,» said Dr. Lyden.

Present in all plasma samples, PFOS levels ranged from 776 to 118,000 parts per trillion.

«We used magnetic beads of 3 micrometers in diameter to pull down the exosomes in plasma samples,» Zeng said.

The Stanford team found, however, that mitochondrial DNA levels were significantly elevated in plasma samples from a small number of HD patients.

Not exact matches

Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.

The paper studied 20 discharges of plasma chosen as a representative sample of those created in PPPL's National Spherical Torus Experiment (NSTX) prior to its recent upgrade.

However, many studies have found the inconsistency of EGFR mutation status in plasma DNA samples as compared to tumor tissue DNA samples.

They took sludge samples gathered from around the country and measured the metal content using a mass spectrometer that can discern different elements as they are ionized in a superhot plasma.

The technology relies on a method Waterloo's Prof. Janusz Pawliszyn developed in the 1990s called solid - phase microextraction (SPME), which uses a solid coating on a sample probe to selectively extract chemical substances from blood, saliva, urine and even plasma.

Malaria researcher Jonathan Kurtis of Brown University in Providence, R.I., and colleagues examined plasma samples and disease histories collected during a 2005 study of 453 Tanzanian youngsters.

By longitudinal analysis blood samples retrieved from persons who have received the Ebola vaccine, the researchers could show that a group of cytokines measured in plasma within seven days of the vaccine injection correlates with antibody responses developed six months later.

The resulting electric field tugged at ions in the plasma, triggering enough collisions that the sample's surface layer began to circulate.

Blood plasma concentrations of naloxone were measured nineteen times for each volunteer during each treatment session, with intense sampling in the first 15 minutes after dosing.

The world's first known case of AIDS has been traced to a sample of blood plasma from a man who died in the Democratic Republic of Congo in 1959

Similar to high - tech centrifuges, the paperfuge was able to separate plasma from blood samples in less than 1.5 minutes, the team reports today in Nature Biomedical Engineering.

«The signals are sampled very quickly and stored on the computer so we can understand what was happening in the plasma.

In each blood sample, the researchers separated the plasma, the liquid part of the blood, from the blood cells.

Yet all blood plasma samples drawn from 125 American alligators across 12 sites in Florida and South Carolina contained at least six of the 15 perfluorinated alkyl acids (PFAAs) that were tracked in the alligator study.

Using samples from the Dominantly Inherited Alzheimer Network (DIAN) study, the researchers found that people who carried the mutation responsible for this form of Alzheimer's also had altered levels of specific lipids in their blood plasma compared to the control group.

Both systems can simultaneously detect many targets such as cytokines, chemokines and inflammatory biomarkers in a single sample that can be serum, plasma and tissue culture supernatants.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

The difficulty in measuring such a small increment in chromosome 21 DNA concentration has recently been overcome with the use of massively parallel genomic sequencing.20 This technique can identify and quantify millions of DNA fragments in biological samples in a span of days.23 Three cohort studies have shown the feasibility of using the technique to identify fetuses with trisomy 21 by analysis of maternal plasma DNA.24 25 26 The sample numbers studied were small because, typically, only a few samples could be analysed in each sequencing run.

Samples matrixes suitable for metabolomics analyses include in vitro: cell lysate and medium, and in vivo: Plasma, serum, urine, CSF, micro-dialysate and tissues

The group has over 10 years» experience with this methodology, with specific expertise in cytokine / chemokine detection in tissue culture supernatants and plasma samples with panels containing up to 42 analytes.

The researchers detected ctDNA in 19 (12 %) of the plasma samples.

The platform can analyze multiple biomarkers quickly and efficiently, as demonstrated by the fact that a total of 458 plasma samples were analyzed in triplicate, with 23 ELISA analyses per sample - replicate, in a single experiment.

Scientists at PNNL and the CDC previously conducted a global proteomics analysis of plasma and serum samples from the DASP and identified five candidates in a sample subset (Metz et al. 2008).

They identified more than 559 lipids that were significantly different (q < 0.05) between control and patient samples, of which 55 were structurally identified by matching to entries in a blood plasma lipid AMT tag database.

Fifty seventh - and eighth - graders from John Witherspoon Middle School in Princeton came to PPPL for a half day on March 4 to become scientists — doing a variety of hands - on science activities, from building a motor to sampling ice cream frozen with liquid nitrogen in a cryogenics demonstration, to watching cool plasma demonstrations of lightning, static electricity and stars.

The first manuscript entitled, Circulating Free Tumor - derived DNA (ctDNA) Determination of EGFR Mutation Status in Real - World European and Japanese Patients with Advanced NSCLC: the ASSESS Study, used samples from the large ASSESS study to evaluate EGFR mutation status by analyzing ctDNA from blood plasma.

The results of the study demonstrated that analyzing ctDNA from plasma is feasible for the identification of EGFR mutations with mutation status concordance in 1,162 matched samples of 89 % (sensitivity 46 %; specificity 97 %; positive predictive value [PPV] 78 %; negative PV 90 %).

The team used a high - tech sorter — an inductively coupled plasma mass spectrometer — that can detect trace amounts of metals to precisely measure the amounts of lead, aluminum, magnesium, iron and other metals in samples scraped from the pipes.

The SMA Foundation initially provided more than 120 plasma samples from SMA patients collected in the Biomarkers for SMA (BforSMA) clinical study for processing on Myriad RBM's DiscoveryMAP

The SMA Foundation initially provided more than 120 plasma samples from SMA patients collected in the Biomarkers for SMA (BforSMA) clinical study for processing on Myriad RBM's DiscoveryMAP ® 250 + biomarker discovery platform.

They are looking for protein indicators of aggressive breast cancer in the tissue and plasma samples.

Plasma was diluted in RIPA buffer (50 mm Tris, pH 7.5, 150 mm NaCl, 5 mm EDTA, 0.1 % SDS, 0.1 % Triton X100, 0.5 % sodium deoxycholate) with protease inhibitors, LDS buffer (Life Technologies), and sample reducing agent (Life Technologies), so that 2 µL of plasma were run perPlasma was diluted in RIPA buffer (50 mm Tris, pH 7.5, 150 mm NaCl, 5 mm EDTA, 0.1 % SDS, 0.1 % Triton X100, 0.5 % sodium deoxycholate) with protease inhibitors, LDS buffer (Life Technologies), and sample reducing agent (Life Technologies), so that 2 µL of plasma were run perplasma were run per lane.

Blood samples taken in one third of the volunteers (n = 1089) were tested for a variety of micronutrients, including hemoglobin, indicative of iron intake, soluble transferrin receptor, serum ferritin, retinol, vitamin C, beta - carotene, alpha - tocopherol, vitamin B6, cobalamin, holo - transcobalamin, plasma folate, RCB folate and vitamin D.

Guinea pigs were dosed with 100 mg of an oxime aceteyltransferase reactivator, and tissue and plasma samples were taken in a geometric time series.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

Our sample galaxies are known to contain an excessive amount of interstellar dust against sputtering destruction in hot plasma filling the interstellar space of elliptical galaxies.

A plasma viral load of > 100,000 copies / ml resulted in successful amplification of all four amplicons for 86 % of the samples, this value dropped to only 46 % for samples with viral loads of < 20,000 copies / ml.

The 39 plasma samples corresponding to the individuals identified in the prospective study were screened for the presence of antibodies to XMRV or other MLVs.

Radioactivity in serial plasma samples was determined by liquid scintillation counting and expressed relative to the number of counts in the circulation 1 minute after injection.

On arrival, the samples were centrifuged (1200 × g, 15 min, room temperature) to separate plasma from buffy coat and red blood cells, and the samples were frozen in liquid nitrogen until they were analyzed.

Quality - control samples were routinely frozen along with study samples to monitor plasma changes as a result of long - term storage and to monitor changes in assay variability.

Aliquots of the 38 plasma samples collected during each 24 - h GCRC admission were pooled as described in Subjects and Methods for measurement of average hourly plasma ghrelin concentrations.