Sentences with phrase «in supernatants»

Furthermore, an increased amount of IL - 2 was present in the supernatants of anti-CD3 — stimulated DKO and Cbl - b − / − — stimulated CD8 + T cells relative to DGKζ − / − CD8 + T cells, which was increased relative to WT CD8 + T cells (Fig. 3E).

Assessment of hASC - CM composition found high expression of various human growth factors (IL ‐ 6, 8, 12, eotaxin, IP10, MCP ‐ 1, VEGF, and TIMP ‐ 1) in the supernatant following the co-culture of hASCs with islet cells, while IP10, eotaxin, VEGF, and TIMP ‐ 1 became increased with time during islet co ‐ culture, suggesting the presence of paracrine cross ‐ talk between islets and hASCs.

Not exact matches

«It's an interesting project because backwash supernatant disinfection is not widely used in Australia,» said Michael Bambridge, Managing Director of CST Wastewater Solutions.

A major NSW regional council has introduced a supernatant recycling system into the filtration plant in the town water supply, helping to conserve potable water and providing substantial water savings over the long term.

Hybridoma supernatants were screened for anti-rGRF activity by use of a pituitary culture assay system that can detect growth hormone - releasing factor in the femtomole range.

The supernatants were discarded, and the tissue was resuspended in DMEM supplemented with 10 % fetal bovine serum, 2 - mm l - glutamine, sodium pyruvate, nonessential amino acids, and a vitamin solution (Life Technologies, Inc., Rockville, MD).

For each pool, 55 µL of rabbit sera and 55 µL of viral supernatant at a concentration of 100 TCID50 were mixed, incubated for 1 hour at 37 °C, and inoculated onto A549 cells in wells of a 96 - well plate as described above.

Both systems can simultaneously detect many targets such as cytokines, chemokines and inflammatory biomarkers in a single sample that can be serum, plasma and tissue culture supernatants.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

The group has over 10 years» experience with this methodology, with specific expertise in cytokine / chemokine detection in tissue culture supernatants and plasma samples with panels containing up to 42 analytes.

Both technologies allow the simultaneous detection of multiple factors in serum, plasma and tissue culture supernatants.

Vaccinia virus (Western Reserve) recombinant for the codon - optimised gp120 gene from HIV - 1 (Bx08) expressing gp120 protein in (VERO) cell culture supernatant.

Our lab uses this kit routinely to to quantify p24 levels in culture supernatant.

The supernatant of apoptotic cells causes transcriptional activation of hypoxia - inducible factor — 1α in macrophages via sphingosine -1-phosphate and transforming growth factor - β.

Stimulation of pericytes with supernatants from either Ang2 overexpressing HUVEC or supernatants from control HUVEC resulted in significantly increased pericyte migration following Ang2 stimulation (Fig. 2c, Supplementary Fig. 4c, d).

In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and AsiIn this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asiin serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asiin the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asiin Africa and Asia.

Cell debris was removed from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.

To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue culture supernatants.

The results revealed that the supernatant of 72 - hour ATRA - treated NB4 cells was sufficient to induce the tyrosine phosphorylation of STAT2 and the endogenous RIG - G level in U3A cells, in comparison with the relative consistent level of total STAT2 (Fig. 3B).

(C) RIPK2 in HCT116 cells were IP with an anti-RIPK2 antibody (A) and the supernatant after IP in (A) was IP in (B) with the same RIPK2 antibody, and in vitro kinase (IVK) assay was carried out on both samples.

Because ATRA could cause IFNα synthesis and secretion in NB4 cells by up - regulating IRF - 1 (23), we thus quantitated the IFNα in culture supernatant of ATRA - treated NB4 cells.

After 24 hours (on day 1) the mixed viral supernatant was removed, the cells were washed twice with PBS and then cultured in fresh MEF medium.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

On day 3 the mixed viral supernatant was again removed, the cells were washed twice with PBS and then cultured in fresh MEF medium.

Single - cell suspension was obtained by filtering the supernatant through a 40 - μm cell strainer, and cell suspension was then gently loaded onto a layer of Histopaque - 1077 gradient (1 × 106 — 3 × 106 cells / mL HistoPaque in a total of 3 - mL volume) and then centrifuged at 400 × g for 30 min at room temperature.

After the supernatant was filtered through 0.45 - μm membrane, it was added to target cells in the presence of 8 μg / ml polybrene (Millipore, Billerica, MA).

In addition, we showed that, although the supernatants from each of the HCMV - infected cultures contained infectious virus, the viral copy number and the quantity and timing of virus production varied among the various organ - specific MSC.

These changes were accompanied by severe EC damage, decreased E-cadherin RNA level, elevated IFN - gamma in splenocyte culture supernatant, and production of significant IgM antibody against intestinal microbiota.

The supernatant, the medium in which the culture is grown, contains a multitude of beneficial byproducts of the growth process, including vitamins, enzymes, antioxidants, and immune stimulators.

«The supernatant decreased the in vitro uptake of calcium by 15 % (p less than 0.001).