Sentences with phrase «in suspension culture»

Indeed, we found that MELK overexpression in non-transformed HMEC - DD cells resulted in increased cell proliferation in suspension culture (Figure 4 — figue supplement 2A).

Indeed, when we assessed these cells in suspension culture, overexpression of MELK significantly increased the proliferation of HMEC cells (Figure 4 — figure supplement 1A).

Both normal and cancer breast stem cells give rise to 3D mammospheres in suspension culture.

In suspension culture, they formed embryoid bodies (EBs), which contained various cell types originating from three embryonic germ layers.

To induce cardiomyocyte differentiation, compact and well - shaped EBs formed in suspension culture containing 10 % of FBS for 6 days were plated in 24 - well plates in the presence of 15 % of FBS.

(b) Neural spheres were formed in suspension culture.

«Our team has learned to grow tomato plants in suspension culture that produce lycopene molecules with a heavier molecular weight.

Not exact matches

Just to work another pop culture reference in there, the «very high» to «low» suspension options will now range from a «depth» of 0 to «20,000 leagues.»

The ironic and indirect ways of affirming and denying — God bless the Czar and keep him far, far away — modes of speaking that are so important for Jewish humanism, are found in Yiddish, a plastic language that hung like a long suspension bridge over the chasm that separated the world of an isolated, vulnerable religious minority from the dangerous Gentile - dominated majority culture.

BREAKING: SyracuseU Chancellor Kent Syverud, in a campus - wide email, has said that in response to the suspension of Theta Tau, SU will be conducting «a top to bottom review of all our Greek life policies, activities and culture, to be commenced this week.»

USA Today examined the NFL's shift in culture in light of helmet - to - helmet hits from Washington safety Brandon Meriweather and Dashon Goldson's suspension for violating safety rules.

In this case, we began with carrot suspension cultures and have already produced small quantities of «heavy - labeled» lutein for animal trials,» Rogers said.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

Microcarriers represent a viable solution by enabling anchorage - dependent stem cells to be cultured in suspension, allowing significant scale - up in bioreactors.

This process (see figure) started with spin - embryoid body (spin - EB) formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.

Suspension culture of hepatocyte - derived reporter cells in presence of albumin to form stable three - dimensional spheroids.

After 6 — 7 days of suspension culture, the neurospheres were replated onto matrigel - coated 60 mm tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

Viral Manufacturing To manufacture viral vectors, two approaches generally are adopted for culturing purposes: scale - out of adherent - cell systems based on 2D planar technologies and scale - up of 3D suspension cell cultures in stirred - tank bioreactors.

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

However, the industry appears to favor cells adapted to suspension cultures (e.g., HEK293), which can successfully grow in serum - free media and thus provide a heightened safety profile and reduced cost of goods (CoG)(33).

However, suspension - adapted production cell lines cultured in large - scale bioreactors are more amenable to therapeutic product expression because of their enhanced scalability and elimination of detachment agents, which facilitates regulatory compliance.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

To derive NSCs as previously described [11], hESC colonies were harvested using a scraper and cultured in suspension as EBs for 8 days in ESC medium minus FGF2.

«Clearly, there is a great need for rigorous evaluation research, which should focus both on the impact of school discipline reforms and on their potential unintended consequences,» the authors note, emphasizing that reducing suspensions is a starting point in effective school discipline reform but that changing school culture can have «spillover» effects on teachers and peers which raise important questions for further study.

Over the past two years, suspensions have declined to one - fifth of the previous figure, thanks in part to a restorative justice program and an emphasis on positive school culture.

Research shows that implementing SWPBIS results in a significant drop in discipline referrals and suspensions, as well as increased academic achievement, lower dropout rates, higher teacher retention, and improved school culture.11

In addition to student proficiency and growth as measured by state tests, the inputs into CORE's School Quality Improvement Index (SQII) include such indicators as suspension and expulsion rates, chronic absenteeism, and school culture and climate surveys administered to students, teachers, and parents.

The report highlights work done in Manley High School under principal Warren Morgan (Cohort 8) to improve school culture and reduce suspensions.

«Our partnership with the Center for Restorative Approaches has helped us reduce our suspension rate to about 0.04 %, helping us create a positive school culture and ensuring all our kids are in school learning.»

As Indiana University researcher Russ Skiba, the leading researcher on school discipline, has pointed out, schools often apply suspensions and expulsions far too inconsistently to be useful in improving school cultures.

The key to reducing suspensions and expulsions is implementing a proactive, preventive schoolwide culture framework like PBIS or SEL, which promote the teaching, modeling, and acknowledgment of desired behaviors in children.

In recognition of the need for an accelerated «culture shift» in the way SFUSD approaches solutions to disruptive student behavior and growing rates of suspensions, the Board of Education adopted Resolution No. 96 - 23AIn recognition of the need for an accelerated «culture shift» in the way SFUSD approaches solutions to disruptive student behavior and growing rates of suspensions, the Board of Education adopted Resolution No. 96 - 23Ain the way SFUSD approaches solutions to disruptive student behavior and growing rates of suspensions, the Board of Education adopted Resolution No. 96 - 23A1.

The buzz in the staff lunchroom can be as telling of school culture as the suspension rate.

In its first semester of use, the urban Title I school reduced the number of in - school and out - of - school suspensions, and improved the school culturIn its first semester of use, the urban Title I school reduced the number of in - school and out - of - school suspensions, and improved the school culturin - school and out - of - school suspensions, and improved the school culture.

But we are by no means done in taking a hard look the policies, community issues and school cultures that lead to inequity in suspensions and expulsions.

Expulsion and suspension were a necessary evil in order to develop a positive school culture.

Developing a school culture in which suspension is the last resort rather than the first is achievable, said Morales, using her own school — which serves a low - income, mostly Latino student population — as a prime example.

For example, one school saw a dramatic improvement in five high - priority metrics included in the culture plan, including a 59 % reduction in the amount of out - of - school suspensions between the 2012 - 13 and 2013 - 14 school years, a 56 % reduction in classroom off - task / disruptive behaviors across nine grade levels, and an increase from 57.2 % to 59 % in Academic Performance Index in just one school year, with a Value Added grade of C for the first time in more than five years.

The district has also seen a 50 % decrease in middle school suspensions in 1 year through dedicated climate in culture efforts, which started with the middle school, and has increased family involvement and gained financial stability.

Enjoy both natural attractions and fine culture in Greenville by exploring the waterfalls at Falls Park on the Reedy, featuring a pedestrian suspension bridge, and heading to the Greenville County Museum of Art, home to works by Jasper Johns, Andy Warhol, and Georgia O'Keefe.

Taken together, the works suggest both a maker and viewer in states of transitory suspension, caught, perhaps, between a life lived directly, without mediation, and reality experienced one step removed, as an observer, or as a consumer of culture.

This work operates with and in the tension between the ethical demand of expression and the institutionalization of taste and sensibility, dwelling on various dysphoric affects of contemporary life — states of suspension, alienation, and impasse — as they manifest in artistic practice and material culture.

... Resuming Fragmented Histories, Steirischer herbst & Künstlerhaus, Halle für Kunst & Medien Last year at Marienbad, Bureau for Open Culture, EFA Project Space, New York, US ArtBO International Art Fair Bogota, CO In the Cut, ACCA Australian Centre for Contemporary Art, Melbourne, AUS The Sea is my Land, MAXXI Museum Rome, Rome, IT En suspension... a Marc Camille Chaimowicz's statement, Frac Carquefou, FR Cinematic Scope, Georg Kargl Fine Arts, Vienna, AT Zero Point of Meaning, Camer Austria, Graz, AT Europen Kunsthalle in Bregenz, KUB Arena Kunsthaus Bregenz, Bregez, AT Speak Memory, Miriam and Ira D. Wallach Art Gallery — Colombia University, New York, US Tomorrow Was Already here, MARCO, MX Sin Motive aparante — For no Apparent Reason, CA2M Centre de Art Dos de Mayo, Madrid, In the Cut, ACCA Australian Centre for Contemporary Art, Melbourne, AUS The Sea is my Land, MAXXI Museum Rome, Rome, IT En suspension... a Marc Camille Chaimowicz's statement, Frac Carquefou, FR Cinematic Scope, Georg Kargl Fine Arts, Vienna, AT Zero Point of Meaning, Camer Austria, Graz, AT Europen Kunsthalle in Bregenz, KUB Arena Kunsthaus Bregenz, Bregez, AT Speak Memory, Miriam and Ira D. Wallach Art Gallery — Colombia University, New York, US Tomorrow Was Already here, MARCO, MX Sin Motive aparante — For no Apparent Reason, CA2M Centre de Art Dos de Mayo, Madrid, in Bregenz, KUB Arena Kunsthaus Bregenz, Bregez, AT Speak Memory, Miriam and Ira D. Wallach Art Gallery — Colombia University, New York, US Tomorrow Was Already here, MARCO, MX Sin Motive aparante — For no Apparent Reason, CA2M Centre de Art Dos de Mayo, Madrid, ES

The Suspension Spike, Changing the Discipline Culture in NYC's Middle Schools — Citing the efforts of multiple JFSF partners and allies, the Office of the NYC Comptroller urged middle schools to adopt restorative practices as an alternative to harsh discipline and stressing the need for support with high expectations and accountability in this report.