Initial evaluation of this method showed 5 — 10 × increase
in transduction efficiency.
Not exact matches
At the end of the project, the
transduction efficiency of the newly made retroviral vectors was tested
in hematopoietic cells, and here my «old» techniques came
in handy.
The use of viral vectors
in research is beneficial for a number of reasons, including but not limited to: helping to get difficult - to - deliver DNA into mammalian cells, increasing the
efficiency of gene
transduction, allowing for control over which cells are infected through viral pseudotyping, and ease of vector cloning and modification.
In general, the higher the passage number of a culture the lower the transfection and
transduction efficiencies.
In addition, this study found that
transduction efficiency varied not only between serum from different manufacturers but also between different lots of serum from the same source.