Engagement of FcεRI results
in tyrosine phosphorylation of kinases and adaptors, and then an increase in intracellular Ca2 + concentration (27, 28).
Specifically, they are interested in those genes that are involved
in tyrosine phosphorylation — the attachment of a phosphate group (a phosphorous surrounded by oxygen atoms) to distinct sites in protein chains where there is a tyrosine residue.
Not exact matches
The sequence similarity between MAD - 3 and pp40 includes a casein kinase II and consensus
tyrosine phosphorylation site, as well as five repeats of a sequence found
in the human erythrocyte protein ankyrin.
At a molecular level, loss of insulin signaling
in astrocytes impaired
tyrosine phosphorylation of Munc18c.
Zhang, Y. and Wolf - Yadlin, A. and Ross, P. L. and Pappin, D. J. and Rush, J. and Lauffenburger, D. A. and White, F. M. (2005) Time - resolved mass spectrometry of
tyrosine phosphorylation sites
in the epidermal growth factor receptor signaling network reveals dynamic modules.
(e, g) Western blot (WB) analysis of
tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP)
in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells.
Fyn membrane localization is necessary to induce the constitutive
tyrosine phosphorylation of Sam68
in the nucleus of T lymphocytes.
C - Terminal
Tyrosine Residue Modifications Modulate the Protective
Phosphorylation of Serine 129 of α - Synuclein
in a Yeast Model of Parkinson's Disease.
Effects of
tyrosine phosphorylation of cortactin on podosome formation
in A7r5 vascular smooth muscle cells.
Tyrosine is an amino acid present
in proteins that contains a hydroxyl moiety, and kinases are enzymes that catalyze
phosphorylation (addition of a phosphate group) of various substrates
in the cell.
Depletion of ABL kinases does not affect YAP1 protein abundance, localization, or
tyrosine phosphorylation in breast cancer cells.
During the past decade, data on the putative roles of STAT proteins
in mediating gene expression without
tyrosine phosphorylation have been accumulating.
The results revealed that the supernatant of 72 - hour ATRA - treated NB4 cells was sufficient to induce the
tyrosine phosphorylation of STAT2 and the endogenous RIG - G level
in U3A cells,
in comparison with the relative consistent level of total STAT2 (Fig. 3B).
Unlike IFNα - activated ISGF3 complex
in which
tyrosine - phosphorylated STAT proteins are required, IRF - 9 could successfully interact with either wt STAT2 or mutant STAT2 - Y690F
in the absence of IFNα, although the interaction between IRF - 9 and wt STAT2 could be obviously enhanced by IFNα via
tyrosine phosphorylation of STAT2 (Fig. 2A).
Similarly, increased level of STAT2
tyrosine phosphorylation was detected as well
in IRF - 1 — transfected HT1080 cells (Fig. 4B).
To exclude this possibility, we constructed a
phosphorylation - deficient STAT6 mutant
in which the potential phosphorylated
tyrosine at position 641 was mutated to a tryptophan (STAT6Y641W).
In this study, we provide the first evidence that in STAT1 - deficient U3A cells, STAT2 forms a complex with IRF - 9 on the ISRE regions of RIG - G promoter and effectively mediates the transcription of RIG - G gene, even without the tyrosine phosphorylatio
In this study, we provide the first evidence that
in STAT1 - deficient U3A cells, STAT2 forms a complex with IRF - 9 on the ISRE regions of RIG - G promoter and effectively mediates the transcription of RIG - G gene, even without the tyrosine phosphorylatio
in STAT1 - deficient U3A cells, STAT2 forms a complex with IRF - 9 on the ISRE regions of RIG - G promoter and effectively mediates the transcription of RIG - G gene, even without the
tyrosine phosphorylation.
The idea that both STAT2 and IRF - 9 were basic components necessary for RIG - G expression was also supported by the fact that ATRA could not only induce the total amounts of STAT2 and IRF - 9 proteins but also increase the
tyrosine phosphorylation level of STAT2
in NB4 cells (Fig. 1A).
Here, we have shown for the first time that the unphosphorylated STAT2 could play an important role
in RIG - G gene expression by interacting with IRF - 9, further reinforcing the idea that STAT proteins could function as transcription factors
in the absence of
tyrosine phosphorylation.
For example, our past work showed that two conserved
tyrosine phosphorylation sites
in the juxtamembrane segment of the Eph receptors not only mediate association with binding partners but also regulate receptor kinase activity.
Twelve - hour exposure of 3T3 - L1 adipocytes to H (2) O (2) or TNF - alpha resulted
in the increase of c - Jun NH (2)- terminal kinase (JNK) activation and insulin receptor substrate 1 (IRS1) serine 307
phosphorylation, concomitantly with the decrease
in insulin - stimulated IRS1
tyrosine phosphorylation and cellular glucose uptake.
Zinc is involved
in insulin signaling by inhibiting the enzyme protein
tyrosine phosphatase to increase
phosphorylation of the insulin receptor.
METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization;
tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1
in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a
in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and
in vitro activity of immunoprecipitated Akt1.