Sentences with phrase «including electron microscopy»

In the current study, the researchers used high - affinity antibodies to «label» the cannabinoid receptors so they could be seen using various microscopy techniques, including electron microscopy, which allowed very detailed visualization at individual synapses, or gaps between nerve cells.

The authors also employed other advanced imaging techniques — including electron microscopy and super-resolution imaging — to discover that the formation, and subsequent loosening, of these contacts is regulated by a lysosomal protein called RAB7.

Before and three months after fat grafting, samples of skin from the treated area were obtained for in - depth examination, including electron microscopy for ultrastructural - level detail.

Several physical measurements, including electron microscopy, assured us that our product was a closed loop coiled tightly around the virus - DNA template and that it was identical in size and other details with the replicative form of DNA that appears in the infected cells.

Not exact matches

These include more sensitive mass spectrometry for detecting, identifying and quantifying protein molecules, electron microscopy for visualizing an assortment of components of the nano - machine that transports toxins, and fluorescence confocal microscopy, which can label proteins with light emitting dyes.

The researchers used a number of methods, including fluorescence and electron microscopy, in collaboration with Dr. Eugenia Klein of the Institute's Microscopy Unit; a unique system in Prof. Alon's lab for simulating blood vessels in a test tube; and in vivo imaging with Prof. Sussan Nourshargh of Queen Mary University of London.

Because they are so small, multiple testing methods are used, including cross-polarized light microscopy, scanning electron microscopy and PCR testing of the DNA of larvae in the water sample.

Therefore, before publication, large data sets (including microarray data, protein or DNA sequences, atomic coordinates or electron microscopy maps for molecular and macromolecular structures, and climate data) must be deposited in an approved database and an accession number or a specific access address must be included in the published paper.

The exchange lists more than 1000 experts in techniques including sequencing, electron microscopy and mass spectrometry.

This work, with the assistance of soil scientists at the University of KwaZulu - Natal, has involved a suite of techniques, including x-ray fluorescence (to provide quantitative data on minor and trace element composition), x-ray diffraction (to reveal crystal structure and parent rock types of paint ingredients), and environmental scanning electron microscopy (to yield qualitative data on elements present).

To make their determination, researchers used a combination of analytical techniques, including atom probe tomography, transmission electron microscopy and electron beam induced current.

Dr. Taraska's lab studies the structural cell biology of exocytosis and endocytosis with advanced imaging methods including live cell microscopy, superresolution fluorescence, and electron microscopy.

For this work, Goodell and colleagues including his collaborator Jody Jellison, now director of the Center for Agriculture, Food and the Environment at UMass Amherst, used a suite of investigative methods including small angle neutron scattering (SANS), sum frequency generation (SFG) spectroscopy, Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), atomic force microscopy (AFM) and transmission electron microscopy (TEM) to fully describe the process.

Using a variety of methods, including nuclear magnetic resonance spectroscopy, calorimetry and electron microscopy, the researchers evaluated the fibers» structural and mechanical characteristics.

Examples of this tradition include the early use of neutron generators in radiation biology and therapy, and the work of the Ruska brothers concerning electron microscopy.

To determine the structural and chemical composition of the soft tissues Lindgren collected and see if the fossil sea turtle did have a dark colored shell, the researchers subjected the sample to a selection of high - resolution analytical techniques, including field emission gun scanning electron microscopy (FEG - SEM), transmission electron microscopy (TEM), in situ immunohistochemistry, time - of - flight secondary ion mass spectrometry (ToF - SIMS), and infrared (IR) microspectroscopy.

Chemical analysis including spectroscopy and electron microscopy of these sheets revealed that they are indeed shell proteins that were preserved for up to 15 million years.

Research problems that are just out of reach today but that could be made accessible by advances in electron microscopy include studies of the little pores that form in our cells walls and which are centrally important in the regulation of all life processes as well as new nano - structured materials that are ultra-light yet strong, allowing reduced energy consumption in vehicles.

Various imaging technologies are used to observe the tiny structures, including fluorescence -, electron - and atomic force microscopy.

Utilizes advanced electron microscopy techniques to study nanoscale structure and defects that determine the utility of functional materials, such as superconductors, multiferroics, and other energy related systems including thermoelectrics, photovoltaics, and batteries.

Ten exoskeleton specimens were studied, and experimental analyses were performed using laboratory techniques including scanning electron microscopy and electron diffraction spectroscopy, revealing details about the structure and chemical composition.

IGFL groups have full access to state - of - the - art core services including high throughput sequencing technologies, X-Ray, microtomography, 2D and 3D imaging, morphometrics, transgenic animal facilities, bioinformatics, mass spectrometry, proteomics, FACS sorting, histology and electron microscopy.

Invited speakers will include leading edge researchers in electron microscopy who are known worldwide for their achievements in materials and biological sciences.

I am an experimentalist, and I use ultrahigh - resolution ion - and electron - microscopy techniques, including focused - ion - beam scanning - electron microscopy and transmission electron microscopy, to determine the composition and structure of these materials at scales ranging from millimeters down to the atomic.

The Calgary Microscopy and Imaging Facility (MIF) is a world - class university - wide facility housing transmission electron microscopy (TEM), scanning electron microscopy (SEM), advanced light microscopy, atomic force microscopy (AFM), including single cell force spectroscopy (SCFS), and advanced image processing for three - dimensional electron and light microscopy, directed by Professor Matthias Amrein.

For higher resolution images and phase identification, transmission electron microscopy (TEM) was employed including energy filtered TEM and electron diffraction to elucidate the elemental distribution and phases throughout the penetrative oxidation.

«Cryo - electron microscopy is one of those techniques so basic and important that its use spans all of biology — including understanding the human body and human disease and in designing new medicines.

The researchers used a novel combination of methods including synchrotron - based X-ray fluorescence microscopy at ANL's Advanced Photon Source and high - resolution electron microscopy analyses at the Environmental Molecular Sciences Laboratory (EMSL) located at PNNL to characterize UO2 - cytochrome interaction.

We work across disciplines and use a variety of techniques including microfluidics, standard microscopies (electron, optical, fluorescence, confocal), spectroscopies (fluorescence, UV, CD), scattering techniques (X-ray, light), protein expression and characterization and cell - free gene expression to investigate the utility of coacervate microdroplets as robust reaction compartments and cellular mimics.

Analyses with scanning electron microscopy revealed Haramiyavia possessed complex teeth that indicated an herbivorous diet, including incisors for cutting and complex cheek teeth for grinding plant food.

Several more researchers presented their work during the day, including protein synthesis at atomic resolution, bio-imaging opportunities at synchrotrons, multi-dimensional imaging during plant cell differentiation, how to use electron cryomicroscopy for in situ structural biology, and how structured illumination microscopy can offer insights into the regulation of mammalian meiosis.

Her many research highlights include the development of new nanomaterials and chemical processes for use in a range of high technology applications, including catalysis, energy, healthcare, chemicals and food coatings, and novel dynamic electron microscopies.

Other areas of instrument development include DNA sequencing, cell fractionation, light and electron microscopy methods, mass spectrometry of proteins, X-ray imaging plates, synchrotron beam - lines and automated cell micro injectors.

He brings a variety of in situ and ex situ characterization methods to bear on the these materials, including high - resolution x-ray and ultraviolet photoelectron spectroscopy, x-ray diffraction, Rutherford backscattering, scanning transmission electron microscopy, electron energy loss spectroscopy, atom probe tomography and scanning probe microscopy.

Such studies integrate several research tools including classic animal dissections, histocytochemistry, immunohistochemistry, electron microscopy, kinematics & motion analysis, electromyography, and other electrophysiological techniques.

To carry out this work, Joshua - Tor has expanded her methods to include cryo - electron microscopy, which allows her to solve large structures at a level of detail not possible in the past.

The positions of 3769 tungsten atoms in a tungsten needle segment were determined to a precision of 19 pm (0.019 nm), including the position of a single atom defect in the interior of the sample, by using aberration - corrected scanning transmission electron microscopy and computerized tomography.

Immunohistochemical labeling for external limiting membrane (ELM) protein markers, including pan-cadherin, β - catenin, and PALS1 (MPP5), is fragmented or diffuse in these mutant mice, and electron microscopy demonstrates a reduced number of adherens junctions.

With the colleagues and resources available at UCSD and the Ludwig Institute, including new capabilities in cryo - electron microscopy, Corbett thinks Spo11 is a problem ready to be solved.

Using an integrative approach that combined electron microscopy on the Env trimer complex with PGT151 (led by the Ward lab) with the structure of the PGT151 Fab by x-ray crystallography (led by the Wilson lab), the scientists were able to visualize the location of the PGT151 - series binding site on the Env trimer — which includes a spot on one gp41 protein with two associated sugars (glycans), a patch on the gp120 protein and even a piece of the adjacent gp41 within the trimer structure — «a very complex epitope,» said Claudia Blattner, a research associate in the Wilson laboratory at TSRI and member of the IAVI Neutralizing Antibody Center who, along with graduate student Jeong Hyun Lee, was a first author of the second paper.

Berkeley Lab researchers, working at the Molecular Foundry, have invented a technique called «CLAIRE» that extends the incredible resolution of electron microscopy to the non-invasive nanoscale imaging of soft matter, including biomolecules, liquids, polymers, gels and foams.

The approaches combine theoretical studies — including statistical physics of non-equilibrium systems — and a variety of experimental techniques such as optical and electron microscopy, as well as microfluidics and micropatterning, optogenetics, or mechanical micromanipulation using optical or magnetic tweezers.

We provide diagnostic services for all animal species, including necropsy, bacteriology, clinical chemistry, electron microscopy, serology, endocrinology, histopathology, immunohistochemistry, virology, parasitology, molecular diagnostics, and toxicology.

Laboratory tests include blood tests (e.g., to detect a low white blood cell count) and other tests to detect the virus (e.g., ELISA, electron microscopy).

Currently he is Director of High Performance Computing Systems and Applications at The Rockefeller University where his work supports applications in areas including cryo - electron microscopy, super-resolution imaging and genomics.

Methods included mass spectrometry (ICPMS), ion - exchange column chromatography, gas chromatography, Raman spectroscopy, scanning electron microscopy, laser ablation, and laser fluorination.

Use tools and methods including solid - state synthesis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and dilatometry.

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign