Sentences with phrase «including immunofluorescence»
Secondary antibodies play an important role in many research applications, including immunofluorescence, immunohistochemistry, Western blot, ELISA, flow cytometry, and more.
http://www.sciencemag.org/ Not exact matches
All genes with Data reliability «Supported» in one or both of the two methods immunohistochemistry and immunofluorescence, or standard validation «Supported» for the Western blot application (assays using over-expression lysates not included) are classified as «Evidence at protein level».
Antibodies used for immunofluorescence staining included YAP1 from Cell Signaling and TAZ from BD Biosciences.
The antibodies used in this study includes COX - 2 (Cayman Chemicals, Ann Arbor, MI, USA), STAT6 for Western blot (Cell Signaling Technology, Danvers, MA, USA), for immunofluorescence (IF)(BD Pharmingen, San Diego, CA, USA), for ChIP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho - STAT6 (Cell Signaling Technology), p300 for Western blot, (Upstate, Lake Placid, NY, USA), for ChIP (Santa Cruz Biotechnology), normal rabbit IgG (Santa Cruz Biotechnology), mouse immunoglobulin (Ig) G1, κ Ig (BD Pharmingen), glyceraldehyde -3-phosphate dehydrogenase (Advanced ImmunoChemical, Long Beach, CA, USA), Cy3 - conjugated AffiniPure Goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) and PARP (Cell Signaling Technology).
They showed pluripotency in many ways, including their expression of pluripotency markers, such as OCT3 / 4, NANOG, SOX2, SSEA1, SSEA4, and AP in immunofluorescence assay, Oct4, Nanog, Sox2, Klf4 and cMyc in RT - PCR.
FIV antibodies can be detected using a number of techniques, including enzyme - linked immunosorbent assay (ELISA), western blot, and immunofluorescence (IFA) assays.
Other diagnostic methods are available including culture, microscopic agglutination test (MAT), immunofluorescence, darkfield microscopy, other serologic tests, and real - time polymerase chain reaction (PCR).