Sentences with phrase «increasing bleomycin»

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Mean γH2Ax foci / nuclei was observed to significantly increase (p < 0.05) with increasing concentrations of Bleomycin (20, 40, 60, 80 μM) in parental HCT - 116 (Fig 1e and 1f).

a) Graph showing dose dependant (20, 40, 60, 80 µM) increase of number of enriched Side Population of HCT 116 post treatment with Bleomycin b) Representative FACS analysed FlowJo images showing enriched side population post treatment with Bleomycin.

c) Graph showing dose dependant (20, 40, 60, 80 µM) increase in CD44 enriched cells post treatment with Bleomycin sulphate (p < 0.001).

Furthermore, on treatment with 80μM bleomycin, a significant (p < 0.05) increase in expression of stemness genes like CD44, Oct 4 and a significant downregulation of CD24 gene (p < 0.05) was observed in clonospheric HCT116 when compared to Parental HCT116 (Fig. 3a and 3b).

To check for the presence of this subpopulation of SP cells, we exposed HCT116 to increasing concentrations of Bleomycin and performed Hoechst efflux assay.

A significant population of cells was observed to efflux Hoechst 33342 dye when treated with increasing concentration of Bleomycin (20, 40, 60, 80 μM)(Fig 1a and 1b).

With increasing dosage of Bleomycin, significantly low foci per nuclei (p < 0.05) was observed in clonospheres as compared to that in parental HCT - 116 (Fig 2a and 2b).

To check for the extent of DSB generation, we treated HCT - 116 with increasing concentrations of Bleomycin and quantified the formation of γH2Ax foci.

Immunocytochemistry also confirmed reduced expression of CD44 in siTRF2 silenced HCT116 cells as compared to Scrambled siRNA when treated with increasing concentration of Bleomycin (Fig 4d).

To further characterize the stem cell like properties of SP cells we treated HCT116 with increasing concentrations of Bleomycin and observed variation in CD44 percentage.

A significant increase in stem cells in terms of CD44 positive population was observed with higher Bleomycin concentration (p < 0.001)(Fig 1c and 1d).

Elevated TRF2 expression in clonospheres at high concentration of Bleomycin may contribute to increased expression of stem cell factors.

siTRF2 transfected cells were treated with increasing concentrations of Bleomycin sulphate (0µM, 40µM and 80µM) and immunocytochemistry was performed as per laboratory standardized protocols [22].

DNA damage, in terms of average olive tail moment, was observed to increase with increasing concentration of Bleomycin in Parental HCT 116 cells.