Sentences with phrase «individual consensus sequences»
After sequence read quality and damage filtering (Supplementary Fig. 4), we called per - individual consensus sequences for nucleotide positions covered by a minimum of two independent reads.
http://www.nature.com/ Not exact matches
Polymorphisms observed in the consensus sequences of all individuals were cross-referenced with the MITOMAP database (http://www.mitomap.org/MITOMAP) of reported disease - associated mutations to determine whether any individuals harbored disease - causing mtDNA SNPs.
In order to measure associations with mtDNA SNPs and haplogroups, mtDNA consensus sequences were identified for each individual.
The resulting consensus sequences averaged 16,231 ± 455 bp, or 98.1 % of the complete mitochondrial genome, with the average number of reads per nucleotide position (fold - sequence coverage) ranging from 7.4 × to 117 × among the 9 individuals (Supplementary Data 1).
mtDNA sequences from the 166 individuals were aligned with published sequences from 17 additional individuals, resolving 52 unique haplotypes from a consensus length of 410 bp.