Sentences with phrase «induce knockdown»
We first designed a protocol for RNA interference (RNAi) to induce knockdown of sema2a.
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Simultaneous knockdown of GBP1 and GBP2 showed more severe growth retardation, and this reduction in growth rate was indistinguishable from that induced by overexpressing TSC1 and TSC2 in the fat body.
However, these reciprocal decreases were much lower than those induced by knockdown of the ABL kinases (Fig. 7).
Further analysis using QPCR demonstrated that the knockdown effect was induced by sema2a dsRNA specifically, and lasted at least 7 days post-deafferentation.
To further validate that the decreased metastasis induced by double knockdown of ABL1 and ABL2 was not due to off target effects of the lentivirus - encoded shRNAs, we used a second set of ABL shRNAs (shAA # 2) and carried out rescue experiments by expressing mouse Abl1 and Abl2 constructs (mAbl1 / Abl2) that are resistant to shRNAs against human ABL1 and ABL2.
Further studies showed that Notch1 knockdown in NSCLC helps induce cell death (which inhibits tumor initiation and growth); this process is mediated by p53, and thus the effects of inhibiting Notch1 require functional p53.
(C) A caspase inhibitor prevents MELK knockdown - induced cell death.
(E) MELK knockdown induces the accumulation of cells with 4n DNA content and G2 / M arrest.