Sentences with phrase «infected cells in culture»

In other experiments, the protein blocked the virus from infecting cells in culture, and it even prevented mice from getting sick when administered either 1 day before or after viral exposure, they report today in Nature Biotechnology.

Scientists have created a designer protein that stops the influenza virus from infecting cells in culture and protects mice from getting sick after being exposed to a heavy dose of the virus.

Not exact matches

Shukla and colleagues discovered that a small drug molecule called BX795, which is sold to labs for use in experiments, helped clear HSV - 1 infection in cultured human corneal cells, in donated human corneas, and in the corneas of mice infected with HSV - 1.

Using human fetal «mini-brains» grown in 3 - D cultures, scientists determined that a specific protein produced by the Zika virus changes the properties of neural stem cells in the developing brain of an infected fetus, potentially causing microcephaly in newborns (Ki - Jun Yoon, abstract 103.06, see attached summary).

The mutated, drug resistant H7N9 virus, however, was still able to infect cultured human cells and spread between laboratory animals as efficiently as nonmutated strains, researchers report December 10 in Nature Communications.

At the Galveston National Laboratory, which houses a biosafety level 4 lab, researchers shut down experiments, euthanized infected animals, put pathogens in cold storage, and autoclaved cell cultures.

In collaboration with Ding, the lab of Olivier Voinnet at the Swiss Federal Institute of Technology in Zurich also reported in an accompanying paper the detection of viral siRNAs in cultured mouse embryonic stem cells infected by the Encephalomyocarditis viruIn collaboration with Ding, the lab of Olivier Voinnet at the Swiss Federal Institute of Technology in Zurich also reported in an accompanying paper the detection of viral siRNAs in cultured mouse embryonic stem cells infected by the Encephalomyocarditis viruin Zurich also reported in an accompanying paper the detection of viral siRNAs in cultured mouse embryonic stem cells infected by the Encephalomyocarditis viruin an accompanying paper the detection of viral siRNAs in cultured mouse embryonic stem cells infected by the Encephalomyocarditis viruin cultured mouse embryonic stem cells infected by the Encephalomyocarditis virus.

Greber and his team infected human cells in culture with the chemically labeled viruses, and observed the behavior of the viral DNA during entry into cells.

The implications were hardly lost on the Bethesda crowd: If the virus was transmitted in cell cultures in Ruscetti's lab, it could also be contaminating the nation's blood supply as a result of blood donations from unknowingly infected donors.

They found that indeed, they do, and that stimulating these cells led them to kill cells infected with HIV - 1 derived from latently infected cells, both in culture and in mice engineered to have a human immune system.

«We compared the ability of RSV and parainfluenza virus (PIV3)-- another common virus in children that causes much less severe airway disease — to infect and cause inflammatory responses in a cell culture model of human epithelial cells, which compose the lining of the lung airway.

The research showed the beta - lactamase genes in Klebsiella pneumoniae were on all the time, whether or not the bacteria were infecting human cell cultures.

They found that the infected cultures responded to the drugs in the same way that liver cells inside the body are known to do.

To find out if the new viruses actually cause disease, researchers can try to grow them in cell culture and infect animals with them.

Then, by placing surface proteins of each HCV virus onto these pseudoviruses, the researchers were able to efficiently infect human cells with the HCV strains in tissue culture.

The new study, led by Walter Mothes, a Yale microbial pathogens expert, involved creating one culture that mixed healthy rat cells with cells infected by the murine leukemia virus, a cancerous pathogen in rats and monkeys that is not known to affect humans.

Another, previously unknown gene — when the investigators worked out its protein's structure and studied it in tissue culture — proved to do just the opposite: The protein helped the schizont leave the infected blood cell.

Five days post-stimulation the anti-CD3 / anti-CD28 coated magnetic beads were removed from each of the three cultures (non-transduced (NTD), AdX4 - ZFNs, and AdR5 - ZFNs) and 2.5 million cells were seeded in each of four cultures that were subsequently infected with either Bk132 (primary X4 isolate), HxB2 (lab - adapted X4 isolate), R3A (R5X4 primary isolate), or media only (mock).

We also found that after 19 days post-HIV infection, reduced but significant cell growth was detectable in several of the HIV - infected control cultures, untransduced and treated with R5 - ZFNs.

D, the same primary breast cancer cells infected with KLF4 or control lentivirus were cultured in low - attachment plates and the number of mammospheres was counted after 10 days.

The tumor cells were dissociated and the cells were infected with KLF4 lentivirus and cultured in low - attachment plates.

C, primary breast cancer cells isolated from patients with advanced cancer were directly infected with KLF4 or control lentivirus, and the CSC population (CD24 − CD44 + ESA +) was measured by FACS after culturing the cells for 72 hours in low - attachment plates.

Virus isolation is usually achieved using in vitro cell culture techniques, but this is not easily achieved from CCD bees because many are infected by multiple viruses and plaque purification techniques are not available for most of these viruses.

Your culture dishes could be infected with Mycoplasma, tiny bacteria that thrive in cell culture environments.

Our study shows that selecting the appropriate cell line, growing the cells as 3 - D aggregates and infecting them when they are fully differentiated is key for successful in vitro cell culturing of human noroviruses.»

Liposome - siRNA - peptide complexes cross the blood - brain barrier and significantly decrease PrPc on Neuronal cells and PrPRES in infected cell cultures.

CRISPR / Cas9 was able to eliminate HIV in infected human T cells in culture.

Five years ago, the group reported that when they infected human tonsil cells with HIV in culture, 95 percent of the CD4 + T cells that died did so before the virus had a chance to reproduce within them.

To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue culture supernatants.

Cells with similar characteristics accumulate during normal aging as well as in younger persons infected with human immunodeficiency virus, suggesting that the process of replicative senescence is not an artifact of cell culture but is also occurring in vivo.

These reporters will give us the ability to sort infected cultures to analyze changes in gene expression in directly infected cells and bystander cells and compare to uninfected cells.