Sentences with phrase «injected with»

As controls, groups of male Tg mice were injected with pancreas homogenate from age - matched non-Tg mice that did not have any detectable IAPP aggregates (Fig. 1).

(A) The fasting blood glucose concentration was measured over time in Tg - hIAPP or WT mice injected with synthetic IAPP aggregates or PBS.

For experimental metastasis assay, nude mice (7 — 8 weeks) were injected with 50,000 luciferase - labeled CSCs in PBS into left cardiac ventricle in a total volume of 100 μL.

Fasting glycemia was measured periodically in Tg - hIAPP mice treated with synthetic IAPP aggregates or PBS as well as in WT mice injected with the same preparation of synthetic aggregates (Fig. 7 A).

The tumors grew rapidly in the control experiments (the median time for tumor - free survival was one week) and any differences in tumor - free survival for the controls and the mice injected with cells expressing mutated PREX2 were not statistically significant (Horrigan et al., 2017).

To analyze insulin responses to glucose challenge, mice were injected with glucose as described previously for IPGTT, and postinjection blood samples were taken at 30, 60, and 120 minutes.

(B) Represents the ratio of organ weight / body weight of animals injected with different inocula.

(B) To examine whether mutant ACVR1 could activate BMP signaling in embryos lacking both Bmp7 and Bmp2b, embryos from crosses of bmp7 homozygous adults were injected with 10 pg mutant ACVR1 mRNA with or without injection of a translation - blocking MO targeting bmp2b.

Fasting blood glucose level became significantly higher in animals injected with Tg - hIAPP pancreas homogenate containing IAPP aggregates, as early as 8 wk compared with mice treated with WT pancreas extracts (Fig. 4 A).

Animals fasted for 16 h were injected with 1 g / kg body weight of glucose i.p., and blood glucose level was monitored subsequently at different time intervals.

After collecting the first sample, mice were injected with 1.0 g / kg glucose in sterile saline [intraperitoneal (IP)-RSB-, and postinjection samples were taken at 30, 60, and 120 minutes.

This effect was not seen when embryos were injected with the same concentration of heat inactivated BSA demonstrating that it is indeed the presence of Mcf1 that causes the freezing phenotype (Video S5).

As a control, group of IAPP Tg mice were injected with PBS.

When mice are fed the masked hexapeptide, the effect is the same as in those that were injected with the unmasked hexapeptide.

Five minutes before injection of [11C] raclopride, subjects were i.v. injected with placebo (3 mL of saline) on one day and with MP (0.5 mg / kg) on another day.

Kaplan - Meier analysis demonstrated that most of the mice injected with cells expressing wild - type PREX2 or GFP exhibited tumor - free survival for more than ten weeks.

(G — L) In situ hybridization of wild - type embryos injected with wild - type (G — I) or mutant ACVR1 (J — L) to detect ventral markers eve1 (G, n = 12/12; J, n = 10/13; onset of gastrulation) and gata2 (H, n = 14/14; K, n = 15/19; mid-gastrulation) or dorsal marker foxb1.2 (I, n = 12/12; L, n = 6/13, 7/13 showed no expression; mid-gastrulation).

The results showed that Tg - hIAPP injected with pancreatic homogenate containing IAPP aggregates (the Tg / Tg group) exhibited an altered islet morphology characterized by the appearance of α - and δ - cells in the center of the islets, compared with the classical pattern in mice (observed in Tg / WT), in which these cells are mostly confined to the periphery of the islets (Brissova et al., 2005).

We found that 70 % of embryos injected with Mcf1 failed to develop to first larval instar compared with 26 % of embryos injected with the same concentration of heat inactivated BSA (Figure 3C).

These abnormalities were not observed during the time of our experiments in Tg - hIAPP untreated mice or mice injected with pancreas homogenate without IAPP aggregates.

Here we report the discovery of an acute inflammatory condition that is mediated by Toll - like receptor (TLR) 2 and TLR6 and which induces hypoferremia in mice injected with TLR ligands.

They gave mice a single intranasal dose of the drug and 2 hours, 24 hours, or 48 hours later injected them with a normally lethal dose of influenza virus.

(E and F) Black bars represent the animals inoculated with old Tg - hIAPP pancreas homogenate, and white bars correspond to the controls injected with WT pancreas.

In addition, rats injected with the drug didn't respond to ankle pressure, demonstrating reduced pain.

And in a follow - up study presented at the American Chemical Society meeting in San Diego, California, this month, Al - Abed and colleagues demonstrated that mice lacking the MIF gene remained healthy after being injected with streptozotocin.

Mice injected with the blocker - treated leukemia cells, however, only exhibited cancer in 2 % of their bone marrow cells.

And when the researchers gave mice manganese and then injected them with shiga toxin, all of the rodents survived.

Of the 15 animals injected with the microRNA blocker, none showed any signs that the treatment was toxic, the researchers report online today in Nature.

Similarly, rats injected with high levels of uridine were equally tenacious.

CDC researchers took ferrets never infected with an influenza virus and injected them with this year's vaccine, which has an H1N1 component of human, not swine, origin.

Before ending the experiment, the mice were injected with a compound that's selectively incorporated into dividing cells, and hence labels adult - born neurons.

Remarkably, Huntington disease animals injected with Rhes experienced an exacerbation of motor issues, including loss of balance and coordination.

By examining the hippocampus — a portion of the brain associated with the process of learning — after the rats learned to associate a sound with a motor response, scientists found that the new brain cells injected with dye a few weeks earlier were still alive in those that had learned the task while the cells in those who had failed did not survive.

When mice were immunized with the vaccine, they produced a 50-fold higher concentration of the potent IgG - type antibodies as compared to mice injected with the standard polysaccharide vaccine.

He had been stripped to his blue jeans, and his stomach had been injected with embalming fluid.

In a memorable scene in The Program, a film based on his fall from grace, he is portrayed being injected with EPO accompanied by the line: «This is science; no longer confined to Earth — now we have learned to fly.»

Human egg cells behaved the same way; when human ovary tissue was grafted into mice injected with PAH, the eggs died, the team reports in Nature Genetics online this month.

They also injected them with chemically labelled water.

When mice were injected with irisin or viruses engineered to express irisin, the team found significant increases in bone volume and thickness compared to mice treated with saline.

Another group of mice were injected with the cancer cells alone.

Twenty - four hours after the injection, the researchers saw large numbers of immune system white blood cells in tissue samples of the rodent brains near the site of injury of those mice injected with the cytokine IL - 1b, but not in the brain tissue of the control group of mice.

In the first study, cell biologist Mark Mayford of The Scripps Research Institute in San Diego, California, and colleagues genetically engineered mice to be able to relive a memory when injected with the schizophrenia drug clozapine.

The study, published in Scientific Reports, demonstrated that animals injected with synthetic DNA engineered to encode a specific neutralizing antibody against the dengue virus were capable of producing the exact antibodies necessary to protect against disease, without the need for standard antigen - based vaccination.

They created a special strain of mice that could be stripped of dopamine and injected with different classes of compounds to try to restore movement.

«We first succeeded in showing that new neuronal cells were produced in the areas injected with the gene.

Mice injected with breast cancer cells making lots of a long strand of RNA called HOTAIR developed 10 times as many lung tumors as controls (left) did.

By contrast, mice who were not intellectually challenged and / or whose activities and diets were restricted, were eager to return to the quarters where they had been injected with cocaine for weeks on end.

When born, mice injected with the miRNAs had the same abnormal behaviour as the stressed mice, whereas those injected with control RNA didn't, coming close to proving that the miRNAs transmitted the abnormal behaviour.

To investigate that, what was needed was a group of people who had been injected with material from another person, and the opportunity to examine their brains in great detail, preferably when they were still relatively young and before they might have spontaneously developed early signs of Alzheimer's.

Scientists collected wild harlequin ladybirds from infested homes — including the home of the mother of one of the authors — and injected some with bacteria or yeast.