By selectively adding or deleting stretches of DNA in the (artificially) fertilized cell, scientists could knock out genes for a disease like diabetes or
insert genes coding for extra height or intelligence.
His trick was to
insert the gene coding for CREB into a version of the herpesvirus that can infect neurons without spreading to nearby cells.
For the purpose of their study, the researchers
inserted a gene coding for fluorescent protein in the worms that, when activated, made them glow brightly under ultraviolet light.
Not exact matches
The researches found that the transgene was
inserted into an active region of the genome, thereby disrupting the
coding sequence and ultimately the function of the plants own OsAux1
gene.
The scientists used CRISPR, a
gene - editing tool, to alter
inserted genes so that the enzymes for which they
coded would work most efficiently amid the exotic acidity, osmotic character and chemical composition of their new home.
However, in the presentations, speakers for both teams said that two stretches of the
gene code for so - called zinc fingers — protein structures that
insert themselves into the grooves of DNA — suggesting that the encoded protein probably turns other
genes on and off.
Scientists from Harvard University, the University of Pittsburgh, and the University of Missouri at Columbia devised another solution:
inserting into pig cells a
gene that
codes for an enzyme that converts omega - 6s to omega - 3s.
In these artificially produced strand breaks, they can
insert or cut out
genes and change the genetic
coding according to their needs.
To determine whether their method had succeeded, the researchers
inserted plasmids carrying a
gene that
codes for a green fluorescent protein.
Interestingly, while viruses certainly have the ability to edit human DNA — most obviously by
inserting their own genetic
code into DNA so that the new viruses are built alongside DNA replication — the review article explains that viruses do not necessarily turn off the immune system by editing
genes.
The group
inserted an algal
gene that
codes for a light - responsive protein into mouse embryonic stem cells.
The team began by
inserting a circular piece of DNA into the mouse
gene that
codes for CFTR.
Karl Deisseroth of Stanford University, and colleagues,
inserted into mice the
gene which
codes for the algal protein ChR2, which caused the protein to attach itself to the surface of nerve cells.
A cellular repair mechanism attempts to rejoin the cut DNA ends, but occasionally
inserts or deletes bases, which turns the DNA
code into gibberish and can knock out a targeted
gene.
To get around this problem, neurobiologists Susana Lima and Gero Miesenböck of the Yale University School of Medicine in New Haven, Connecticut,
inserted a
gene that
codes for a rat ion channel into Drosophila fruit flies.
Another difficulty is that the
gene that
codes for dystrophin is too large to fit into the retroviruses that scientists use to
insert genes into cells.
An adult C. elegans with GFP
coding sequence
inserted into a histone - encoding
gene.
To accomplish this, we used homologous recombination to ablate nonmuscle myosin heavy chain (NMHC) II - B by
inserting cDNA encoding green fluorescent protein (GFP)- NMHC II - A into the first
coding exon of the Myh10
gene, thereby placing GFP - NMHC II - A under control of the endogenous II - B promoter.
With his bioengineering experience, Denby knew that it was possible, though difficult, to isolate the
genes that
code for those two flavor molecules and
insert them into brewer's yeast, the microbe responsible for fermenting barley into beer.
They did so by
inserting mutations into the bacterial
gene that
codes for the enzyme, then subsequently selecting mutants that were particularly effective at clipping off the antigens.
In conjunction with these strains, several cre transgenic strains have been created where BAC engineering was used to
insert an intron containing cre or creERT2 cassettes, followed by a polyadenylation sequence to terminate transcription of the fusion transcript immediately after the recombinase
gene, into the BAC vector at the initiating ATG codon in the first
coding exon of the
gene.
While genetic engineering involves the exchange of
genes between species, synthetic biology involves artificially creating new genetic
code and
inserting it into organisms.