Sentences with phrase «interfering rna»

What if we could use small interfering RNA to block HIV infection in humans?

In collaboration with Nanyang Technological University, experiments were also conducted to co-deliver paclitaxel and small interfering RNA (siRNA) targeting a protein that prevents cell death (Bcl - 2) to MDA - MB - 231 human breast cancer cell line.

Nanoparticles carrying drugs or short interfering RNA have shown great potential for the treatment of a variety of diseases, including cancer.

Transfecting the cells with a small interfering RNA (siRNA) designed to degrade the messenger RNA (mRNA) for CD4 indeed blocked HIV infection by 4 - to 10-fold.

When the goal is to block the expression of genes with molecules of siRNA (small interfering RNA), the synthetic DNA can be designed with a section complementary to the RNA so that the RNA will loosely attach to it.

To make small interfering RNA (siRNA) against IRF - 1, 19 - bp sequence (5 ′ - CTTCCAGGTGTCACCCATG - 3 ′) within the coding region of IRF - 1 gene was selected and cloned into the pTER + vector (22).

Forced expression of fascin1 in colorectal cancer cells increased their migration and invasion in cell cultures and caused cell dissemination and metastasis in vivo, whereas suppression of fascin1 expression by small interfering RNA reduces cell invasion.

In his most recent research, Guo and colleagues detail multiple approaches for the construction of a functional 117 - base pRNA molecule containing small interfering RNA (siRNA).

He's tinkering with small interfering RNA to see how to make them more stable and effective as drugs.

We also show that inhibition of mTOR with rapamycin, as well as silencing mTOR gene product expression using mTOR - specific small interfering RNA, decreased phosphorylation of mTOR signaling proteins and induced cell cycle arrest and apoptosis in ALK + ALCL cells.

Dr. Huizing demonstrated that elimination of the single mutant allele using a synthetic small interfering RNA (siRNA) rescued the abnormal phenotype in cultured cells from sialuria patients.

Inhibition of mTOR and AKT1 expression with small interfering RNA.

The sequences of small interfering RNA (siRNA) targeting the human mTOR, AKT1, and 4E - BP1 gene products were purchased from Ambion, Inc. (Austin, TX) and were as follows: mTOR sense GGAGUCUACUCGCUUCUAUTT and antisense AUAGAAGCGAGUAGACUCCTC, AKT1 sense GGGCACUUUCGGCAAGGUGTT and antisense CACCUUGCCGAAAGUGCCCTT, and 4E - BP1 sense GGUACCAGGAUCAUCUAUGTT and antisense CAUAGAUGAUCCUGGUACCTC.

We design short interfering RNA (siRNA) molecules that are able to trigger the RNAi pathway and mediate the degradation of specific target messenger RNAs (mRNAs), resulting in the expression of a particular disease - causing gene being inhibited.

Biodistribution of small interfering RNA at the organ and cellular levels after lipid nanoparticle - mediated delivery.

Effective gene suppression using small interfering RNA in hard - to - transfect human T cells.

A secretagogue — small interfering RNA conjugate confers resistance to cytotoxicity in a cell model of Sjögren's syndrome.

Noninvasive Imaging of Lipid Nanoparticle — Mediated Systemic Delivery of Small - Interfering RNA to the Liver.

Sequence - specific potent induction of IFN - α by short interfering RNA in plasmacytoid dendritic cells through TLR7.

«We used short interfering RNA, which interact with matrix RNA (mRNA) of the influenza virus.

The delivery of microcontainers with short interfering RNA is ensured within the human body through respiratory tract — nasal sinuses.

Various types of small RNA molecules, including microRNAs, small interfering RNA and, most recently, tRFS, have been implicated in health and disease.

«In mammals, in the absence of haploid cells, other approaches have been used to identify key genes, such as interfering RNA, but they are sub-optimal methods.

First a Nobel Prize, and now a potentially viable treatment: the world's first interfering RNA drug could be the first of many

As the insect pest dines on the plant, the double - stranded RNA gets converted into small interfering RNA.

Snippets of RNA known as short interfering RNA (siRNA) turn off specific genes inside living cells by destroying the messenger RNA molecules that carry DNA's instructions to the rest of the cell.

«We designed a nanocarrier to deliver two passengers: (1) miR - 34a, which degrades hundreds of oncogenes; and (2) a PLK1 small interfering RNA (siRNA), that silences a single gene,» Prof. Satchi - Fainaro says.

Cells in most animals and plants use short - interfering RNA (siRNA) segments to inhibit the formation of viral proteins; here siRNA designed to target IAPV would be fed to colonies as part of double - stranded RNA mixed into a syrup.

Scientists have begun designing their own short interfering RNA against specific viral targets.

Then the researchers squirted short interfering RNA into their noses.

When the team used fragments of interfering RNA to sabotage the production of beta - catenin in these stem cells, the blood cells returned to an early leukaemic state.

In further test tube experiments, the researchers treated the immortalized white blood cells with both chemical and genetic blockers, called small interfering RNA (siRNA), designed to render either p70S6K or GRB10 nonfunctional.

The bits, called short interfering RNA, then associate with other enzymes to form a large complex that can silence certain genes and render the virus harmless.

This multimeric complex localized to primary cilia and was disrupted in cells transfected with β - arrestin small interfering RNA.

This involves gene therapy using a modified virus that makes the interfering RNA only in the presence of an antibiotic.

Researchers deliver small interfering RNAs loaded into nanoparticles into the ears of adult guinea pigs to regenerate hair cells damaged by noise.

The role of miRNAs and of their close cousins small interfering RNAs (siRNAs) in RNA silencing is discussed by Zamore and Haley (p. 1519), and illustrated in the poster pullout in this issue and in research showing that miRNAs can repress the initiation of translation (p. 1573) and, intriguingly, can also increase mRNA abundance (p. 1577).

One of the goals of the Center for Chromosome Biology is to establish screening techniques using small interfering RNAs (siRNAs) to find new targets for anticancer drugs, as well as gain a better understanding of fundamental cancer mechanisms.

Replication of viral RNA leads to high amounts of dsRNA which is recognized by the host's RNAi system and chopped up into smaller RNA fragments, called siRNAs (small interfering RNAs).

In recent years, some biologists have begun studying how these so - called small interfering RNAs (siRNAs) can be used to squelch disease.

Our cells rely on RNAi — deploying diminutive RNA molecules such as microRNAs and small interfering RNAs (siRNAs)-- to turn down activity of specific genes.

Remove the suppressor protein from the virus, Ding's research discovered, and the subject's body will quickly eliminate the virus using the RNAi process, which sends out small interfering RNAs (siRNAs) to kill the disease.

As proof - of - principle of the potential efficacy, Zhang's team grew human ovarian tumors in immunocompromised mice, then injected short - interfering RNAs to block the tumors» growth using RNA interference against FAL1.

«The discovery of small interfering RNAs just got the Nobel Prize in Medicine this year, and now building off that is the possibility of helping millions affected by this major health problem,» says Stephen Rose, chief research officer for the Foundation Fighting Blindness.

The team were able to distinguish between these two mechanisms by using smaller interfering RNAs (siRNAs) to either specifically stop the non-coding RNA from being made, or to degrade the RNA immediately after it was made.

For the new study, Geisbert and his colleagues used snippets of so - called small interfering RNAs (siRNA), which can tinker with a virus's replication.

RNAi silences genes using small pieces of RNA called small interfering RNAs (siRNAs).

Specific inhibition of AQP1 water channels in human pulmonary microvascular endothelial cells by small interfering RNAs.

With the microRNA signatures of mitochondrial function and dysfunction in RPE cells in hand, combined with a detailed mechanistic understanding of how these microRNAs are transferred from cell to cell, we can use small interfering RNAs to target specific microRNAs and block their adverse effects on mitochondria function and their transfer to other RPE cells.

We are investigating the cellular and genetic damages that transposons can inflict on the male germ line, as well as the cooperation of different levels of transposon control, including specialized small interfering RNAs.