In the Intestine Chip, the epithelium forms villi - like projections lined by polarized
epithelial cells that undergo multi-lineage differentiation similar to that of
intestinal organoids, however, these cells expose their apical
surfaces to an open lumen and interface with endothelium.
Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q - real - time PCR; immune response by in - vitro antigen - specific T - cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by surface staining of live bacteria with serum followed by FACS
Intestinal permeability was assessed by Ussing chamber;
epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q - real - time PCR; immune response by in - vitro antigen - specific T - cell proliferation and cytokine analysis by cytometric bead array;
intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by surface staining of live bacteria with serum followed by FACS
intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against
intestinal microbiota by surface staining of live bacteria with serum followed by FACS
intestinal microbiota by
surface staining of live bacteria with serum followed by FACS analysis.
Another issue is that mucus is essential for immunity at
epithelial surfaces, and glycosylation is essential for the integrity of cellular junctions and tissue barriers such as the
intestinal and blood - brain barriers.