So in the new study the researchers slipped the «alien» pair of bases
into bacterial genes that also contained traditional bases.
They did so by inserting mutations
into the bacterial gene that codes for the enzyme, then subsequently selecting mutants that were particularly effective at clipping off the antigens.
Not exact matches
The animals were five times as likely as regular mice to go
into shock and die when exposed to
bacterial cells, the group reports in the November 15 issue of
Genes and Development.
Three options are on the table: tweak cereals so that they form symbiotic partnerships with rhizobia as legumes do; colonise cereal roots with other types of nitrogen - fixing bacteria; or transfer the
bacterial genes that make fertiliser directly
into the crop plants.
A plasmid, a simple kind of DNA - delivery vehicle, will move a
gene for antibiotic resistance
into the
bacterial cells, jump - starting the Crispr - Cas9 system.
After just a single dose, rat intestinal cells pumped out
bacterial lactase for up to 6 months — showing that the gut cells had inserted the
gene into their DNA and were using it to manufacture the enzyme.
Bacteria expressing enzyme in one cell (bright green), while genetically identical cells do not, remaining protected from antibiotic onslaught; image courtesy of Yuichi Wakamoto / Neeraj Dhar / John McKinney Some strains of nasty
bacterial infections, such as MRSA (methicillin - resistant Staphylococcus aureus), come loaded with resistance to antibiotics built right
into their
genes.
To supply lactase over the long haul, Matthew During and his colleagues at Jefferson Medical College in Philadelphia devised a strategy for incorporating the
bacterial lactase
gene into intestinal cells.
B: Well, we were in the midst of experiments aiming to use an animal virus to introduce new
genes into human cells and
into bacterial cells.
A
gene associated with both protection against
bacterial infection and excessive blood clotting could offer new insights
into treatment strategies for deep - vein thrombosis — the formation of a harmful clot in a deep vein.
The team cut and pasted a bovine
gene for NRAMP1, a protein linked to resistance against TB and other
bacterial infections,
into fetal dairy cow genomes.
Beginning in the 1970s, cloning techniques enabled researchers to create multiple copies of a
gene by inserting it
into a
bacterial colony.
They then determined that those vital
genes work together to transport a nutrient
into the
bacterial cell and chop up the nutrient so it can be used as food.
«We want to dig
into this further and figure out how these ions impact
bacterial gene expression, but that work is still underway.»
The islands are now free to move
into other
bacterial cells, bringing with them new sets of
genes.
But people often reject ideas which don't fit
into the
bacterial model of
gene regulation, which is that
genes are turned on and off by proteins binding to DNA.
But Sauer fears that some companies, including those making industrial enzymes using natural
bacterial genes spliced
into E. coli, may find that their intellectual property has suddenly become worthless.
Based on
bacterial genes, the team identified a human protein, selenium binding protein 1, which can convert methanethiol
into other and compounds.
Bacteria share DNA with other organisms with which they come
into contact, so an antibiotic - resistant
gene can transfer from one type of bacterium to another, and a
bacterial cell can become resistant to numerous antibiotics at once.
The new method circumvents the problem of not being able to culture many of these bacteria in the lab by transferring
genes from these bacteria
into another
bacterial species that is easier to work with.
In one of the most elaborate feats of synthetic biology to date, a research team has engineered yeast with a medley of plant,
bacterial, and rodent
genes to turn sugar
into thebaine, the key opiate precursor to morphine and other powerful painkilling drugs that have been harvested for thousands of years from poppy plants.
They injected DNA coding for a
bacterial phytase
gene into more than 4000 pig embryos, 1 % of which incorporated the
gene.
Last April his team reported that they had pieced together
bacterial, yeast, and wormwood
genes and converted yeast
into a chemical factory, yielding artemisinic acid.
These mutations are inserted
into batches of yeast or
bacterial cells, which express the altered
gene and produce millions of random protein variants.
One could incorporate a metagenomic plasmid library
into a probiotic strain and introduce the strain
into a complex host —
bacterial community to isolate
genes that increase the strain's fitness in vivo.
Phage elements, including those not integrated
into chromosomes, are part of a
bacterial system for regulating
genes.
For instance, some of these phage elements can activate or silence
bacterial genes by moving
into or out of the chromosome.
If a soil sample contains
bacterial genes that produce enzymes used to convert carbon dioxide from the atmosphere
into solid carbon, and these
genes are active, researchers can conclude that microbes within the sample are actively storing carbon.
BAC stands for
bacterial artificial chromosome, referring to the way the HD
gene was put
into the mouse.
The
bacterial expression vector pGEX - 5 × -1 / Sp1 was constructed by introducing an EcoRI / XhoI — treated Sp1
gene fragment
into EcoRI / XhoI — treated pGEX - 5 × -1 vector.
Phylogenomic analysis identifies evidence for lateral
gene transfer of
bacterial genes into the E. histolytica genome, the effects of which centre on expanding aspects of E. histolytica's metabolic repertoire.
Over the next few years
genes from different
bacterial (and eventually mammalian) species were cloned
into plasmids and new cloning vectors such as pBR322, pACYC, and pUC were developed to provide higher copy number vectors that could be used in these cloning experiments.
Her game - changing technology takes a mysterious
bacterial genetic code and transforms it
into a powerful tool for cutting and pasting bits of genetic material — meaning not only could the entire field of
gene therapy be revived, but her genome - editing tool could one day be used to treat a range of diseases, from cancer and AIDS to hereditary disorders like Down syndrome and Huntington disease.
A further approach is to introduce
into the human body naked DNA — HIV
genes that are sown
into a circle of harmless
bacterial DNA.
For example, a
bacterial phytase
gene has recently been inserted
into yeast for commercial production.