Sentences with phrase «into blastocysts»

Cells with normal karyotype were injected into blastocysts isolated from C57BL / 6 mice.

Injection of mouse embryonic stem cells into blastocysts / Service Request form plus documentation.

The resulting pluripotent cells were inserted into blastocysts (developing embryos), and the embryos were implanted into surrogate mothers.

Importantly, iPS cells extensively integrate with various host tissues and contribute to the germline when injected into the blastocysts.

We offer quality custom generation of a wide range of mouse models developed by microinjection of Sv129 / Pas or C57BL / 6N ES cells into blastocysts or by DNA pronuclear injection.

The scientists also injected dye - tagged cells into blastocysts, which they inserted into female mice.

Only a quarter of the embryos Hwang cloned developed into blastocysts — hollow balls of roughly 100 cells.

Such deceived eggs continue dividing into the blastocyst stage of 50 to 100 cells before petering out naturally.

During the first week of fertilisation, the embryo grows from a single cell into a blastocyst, a hollow cluster of a few hundred cells.

Then the ES cells have to be injected into a blastocyst, followed by mouse breeding to «go germline.»

About four days after fertilization, that ball has developed into a blastocyst, and many of the cells within it are embryonic stem cells.

Not exact matches

Blastocyst transfer is a procedure allowing the transfer of fewer embryos into the uterus while maintaining high pregnancy rates and reducing the risk of high order multiple pregnancy (triplets and greater).

The rapidly dividing ball of cells — now known as a blastocyst — has begun to burrow into the uterine lining (endometrium).

Only healthy blastocysts are implanted into the mother.

Also critical: blastocyst transfer [allowing embryos to develop robustness in a lab for five days instead of the usual three before transfer back into the uterus].

Fishel's team filmed 88 newly fertilised eggs from 69 couples in their incubator until they become blastocysts — the small ball of cells that is implanted into the womb.

An embryo donated for research revealed two inner cell masses (ICMs, internal cluster of cells at the embryonic pole of the blastocyst which develops into the body of the embryo), which is a sign of monochorionic / diamniotic pregnancy that will give rise to MZ twins.

We take a human embryonic stem cell, and we inject it into a monkey blastocyst [the first 130 or so cells in a newly formed embryo].

Now you know that if you take a mouse embryonic stem cell that is, say, labeled so all of the developed cells will be blue, and you inject it into a mouse blastocyst, you'll get a chimera.

They found that some sperm DNA methylation regions were also associated with poor blastocyst stage of embryo quality as defined by the IVF clinic's standards related to embryo quality before transfer into the uterus of the female partner to establish a pregnancy, Pilsner says.

The patent gives California - based Geron Corp. exclusive rights to animal embryos prepared by transferring the nucleus of a quiescent diploid donor cell into a suitable recipient cell up to and including the blastocyst stage.

Twenty percent of the cells cloned in this way grew into early embryos, called blastocysts, and 5 percent of them yielded embryonic stem cells, which is comparable with results obtained from unfertilized eggs.

His group began with 242 eggs, but after removing the eggs» nuclei and swapping in those of somatic cells, the investigators produced only 30 blastocysts (week - old embryos), from which they coaxed just one stem cell line into being.

Embryos normally implant at the blastocyst stage — when they have divided into about 100 - 200 cells — and it is and it is known that blastocysts have a much greater chance of implanting successfully and resulting in an ongoing, viable pregnancy.

«They looked into expression of mtDNA encoded genes and found no difference between ePNT and control blastocysts.

This was often done using blastocysts from IVF clinics that would otherwise be discarded (often because the blastocysts were damaged and would not properly develop into a human, although IVF generates a large number of excess healthy blastocysts that go unused as well).

The initial single cell combination is already dividing and by the time of entry into the uterus, the egg might have already reached the blastocyst stage.

Approximately ten to fifteen iPS cells were injected into the cavity of each blastocyst, all of which were kept in KSOM with amino acids until embryo transfer.

At three to five days, the embryo develops into a ball of cells called a blastocyst.

Live mice are supplied from a production colony, from a colony recovered from cryopreservation, or via micro-injection of a cell line into host blastocysts.

HV cells cultured under self - renewing conditions were subjected to flow cytometry to separate Venus positive and negative ES cell subpopulations and injected into Rosa26 LacZ expressing blastocysts within 1 h of purification.

Initially we injected purified fractions of HV ES cells into Rosa26 blastocysts that constitutively express β - galactosidase (β - gal) and examined embryos at 9.5 dpc for ES cell (β - gal negative) contribution (Table S4 and Figure S5).

Most strikingly when introduced back into morulae or blastocysts, the V+S + population is not effective at contributing to the epiblast and can contribute to the extra-embryonic visceral and parietal endoderm, while the V − S + population generates high contribution chimeras.

Injected or aggregated blastocysts were then transferred into pseudopregnant recipient mothers.

The table shows the numbers of resultant embryos scored as low -, medium -, and high - contribution chimeras following the injection of fractionated HV cells into Rosa26 LacZ blastocysts.

When injected into host blastocysts, cells derived directly from the ICM of an expanded blastocyst stage can contribute to the PrEn as well as the fetus [17], [18].

The pluripotent properties of ES cells can be demonstrated by in vitro differentiation or by reintroduction of these cells back into chimeric embryos by blastocyst injection or morula aggregation.

We show that the fraction of cells present within this state is influenced by factors that both promote and suppress primitive endoderm differentiation, but conditions that support ES cell self - renewal prevent their progression into differentiation and support an equilibrium between this state and at least one other that resembles the Nanog positive inner cell mass of the mammalian blastocysts.

Embryos that reached the blastocyst stage were transferred into the oviduct of Day 0.5 CD1 pseudopregnant females [16].