Sentences with phrase «into embryonic mice»

They first inserted each enhancer into embryonic mice to learn whether it really did turn genes on.

Not exact matches

Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3 / 4, Sox2, and Klf4 and the other containing the c - Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras.

The team inserted this «magnet» into mouse embryonic stem cells, next to a gene that promotes a protein called p16.

Now you know that if you take a mouse embryonic stem cell that is, say, labeled so all of the developed cells will be blue, and you inject it into a mouse blastocyst, you'll get a chimera.

• A team led by Ron McKay at the National Institute of Neurological Disorders and Stroke in Bethesda, Maryland, turned mouse embryonic stem cells into isletlike cells, another step down the long road to a cure for diabetes.

Beginning with mouse egg cells, Daley and his team tricked these egg cells, or oocytes, into thinking they had been fertilized (a process called parthenogenesis) and managed to isolate embryonic stem cells from the subsequent early mouse embryos.

A tooth grown from embryonic cells has been successfully transplanted into the jaw of a mouse.

Similarly, the three research teams that last week reported turning mouse skin cells into embryolike cells say they will have to study embryonic cells to learn how to reprogram human cells in the same way and to understand their potential.

When he injected mice with embryonic stem cells genetically identical to the mice's own tissues, the new cells thrived, growing into a large clump of adult tissues.

When they transplanted embryonic brain cells into mice that had already been conditioned to fear a sound, the researchers found these mice were significantly more responsive to extinction training.

The group inserted an algal gene that codes for a light - responsive protein into mouse embryonic stem cells.

They found that the combination of the transcription factors Brn3a plus Ngn1, or Brn3a plus Ngn2, reprogrammed a significant percentage of the embryonic mouse fibroblasts into what looked — and acted — like mature DRG - type sensory neurons.

McKay announced that he had mitigated the symptoms of Parkinson's in rats by using mouse embryonic stem cells that he had turned into dopamine - producing brain cells.

Three teams of scientists reported earlier this year that they had directly reprogrammed adult mouse skin cells into embryonic cells, although the process involved viruses and cancer - causing genes.

The current research team, which includes Dr. Minoda and Dr. Hiroki Takeda of Kumamoto University, and several researchers from Keio University, have successfully grafted human iPS - derived cells into the inner ear of embryonic mice, a feat with a high level of technical difficulty.

Smith and Martello plan to encourage mouse embryonic stem cells to turn into neurons, using Microsoft's formal analysis tools as a guide.

There is promising news today for those who hope to turn the potential of undifferentiated stem cells into medical miracles: Researchers are reporting a way to produce insulin - producing cells from mouse embryonic stem cells.

Studying mouse embryonic stem cells, they removed Grb2, a protein essential to the ability of the stem cell to transform into other cell types, from the cells.

They have learned how to turn on and off essential genes and how to transform embryonic stem cells into retinal cells, which can be transplanted into mice to restore vision.

The team injected about 2000 mouse embryonic stem cells each into the brains of 25 rats who had previously had their dopamine - producing neurons damaged, which causes a characteristic tendency to move in circles.

They then transplanted progenitors of the inner ear cells into the inner ear of embryonic normal and Connexin 30 knockout mice using glass tubes with optimized tip sizes.

Mouse embryonic stem cells were turned into something resembling spermatids, the round cells that mature into sperm, by Jiahao Sha of Nanjing Medical University in China and his team.

Furthermore, transplantation into the inner ears of embryonic mice was successful.

Mouse embryonic stem cells injected into rat brains express the AHD2 protein marker (yellow) characteristic of cells lost in Parkinson's disease.

A team of German scientists reported today that it has succeeded in turning stem cells that become sperm in mice into cells with many of the characteristics of embryonic stem (ES) cells.

When the FGF8 gene was injected into or removed from the front part of the brains of embryonic mice, structures toward the front became elongated or foreshortened, respectively.

The researchers did this by injecting the cells into embryonic pancreatic tissue taken from mice engineered to lack neurogenin 3.

During the next five years, KOMP2 will transform the knockout mouse embryonic stem (ES) cells into adult mice for 2,500 lines of well - characterized knockout mice strains, and IMPC will create about 2,500 additional knockout mouse strains.

To appreciate the difference, consider that the old way involves introducing DNA into mouse embryonic stem cells, and then selecting for the rare cells that take up and incorporate the DNA in the right way.

We discovered that mouse mesenchymal stem cells (MSCs) can either fuse and form heterokaryons (cells with 2 nuclei) with mouse embryonic stem cells (ESCs) or can be invaded by ESCs through the entotic process (when one cell enters into another cell).

Lamba and colleagues recently demonstrated that human embryonic stem cells could be directed to differentiate into photoreceptors and respond to a light stimulus as measured by ERG in crx − / − mice (a model for Leber's congenital amaurosis)[5].

When transplanted to the subretinal space of mice lacking functional photoreceptors, human embryonic stem cells directed toward a retinal lineage integrate into the outer nuclear layer, express photoreceptor markers, and restore a light response as determined by the electroretinogram (ERG)[5].

It has recently been demonstrated that mouse and human fibroblasts can be reprogrammed into an embryonic stem cell - like state by introducing combinations of four transcription factors.

They successfully converted mature mouse skin cells into what they called induced pluripotent stem (iPS) cells that had the same wide - open potential as embryonic stem cells — which the researchers showed by turning their iPS cells into nerve and connective tissue cells.

A second method involves introducing the transgenic DNA into embryonic stem cells (ES cells) derived from a mouse embryo at the very early stages of development.

Maturation of human embryonic stem cell - derived pancreatic progenitors into functional islets capable of treating pre-existing diabetes in mice.

(6) Moreover, subcutaneous adipose of prematurely - aged transgenic mice exhibited high levels of staining for the senescence marker senescence - associated - β - galactosidase (SAβ - gal) and expressed high levels of several established markers of senescence, including p21, p19, interleukin - 6, (insulin - like growth factor binding protein - 2 (Igfbp2), and Pai - 1; primary BubR1H / H; INK - ATTAC mouse embryonic fibroblasts forced artificially into senescence by oncogenic Ras or serial passage exhibited a subpopulation that was both GFP + and stained positively SAβ - gal.

Moreover, the iPS cells can spontaneously differentiate or be induced into various cell types of three embryonic germ layers in vitro and in vivo when they are injected into immunodeficient mice for teratoma formation.

Starting with transplants of human oligodendrocytes in the late 1980s [40], and more recently with populations of human oligodendrocyte progenitor cells isolated from the developing or adult CNS, or from human embryonic stem cells, it has been possible to generate extensive myelination upon transplantation into spinal cord injury or into congenital mouse models of hypomyelination [41]--[48].

They have injected stem cells (adult, embryonic) into mice with damaged hearts.

In this study, the team delved deep into the nucleus of cells belonging to mouse and zebrafish embryos — two important animal models of embryonic development — in order to determine how the Dll4 gene is turned on.

Electroporation of investigator - provided construct into mouse embryonic stem cells / Service Request form plus documentation.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

Injection of mouse embryonic stem cells into blastocysts / Service Request form plus documentation.

But researchers did know the genetic mutation that induced it, so Capecchi inserted that mutation into the embryonic stem cells of mice, then coaxed those cells into becoming embryos.

Forbes, Robert Langreth, February 20, 2008: In progress toward a stem - cell treatment for diabetes, researchers at a small San Diego biotech company have devised a procedure for turning human embryonic stems cells into insulin - producing cells inside mice.

These genes normally guide embryonic heart development; however, in the adult mice hearts, they helped transform non-beating cells that form scar tissue into beating heart muscle cells.