Sentences with phrase «into retroviral»

CCR5 cDNA was subcloned into the retroviral vector pBABE - puro.

CXCR4 cDNA was subcloned into the retroviral vector pBABE - puro.

The cDNA library was packaged into retroviral particles and introduced into the J - Lat 6.3 cell line via infection (Table 1).

Once a retrovirus has infected an organism, it commandeers that organism's genetic machinery, turning a once - healthy cell into a retroviral powerhouse that spreads the infection to more cells in an irreversible cascade.

Not exact matches

They assemble themselves like Lego blocks into bulbous retroviral particles, which ooze from the cells producing them.

«Conventional techniques of producing transgenic animals, such as microinjection of genes into eggs and the retroviral transduction of genes into embryos, often produce many animals that are mosaic, which means they do not contain the foreign gene in all their cells.

We also performed qRT - PCR to determine whether the retroviral OCT3 / 4 and KLF4 transgenes were silenced, which has been shown to be a reliable measure for efficient reprogramming of somatic cells into pluripotency [29].

This report suggests it may be possible to reprogram human NSCs into iPS cells by OCT3 / 4 and kenpaullone, further minimizing the undesired effects of utilizing retroviral vectors.

All JAK1 and JAK2 mutants were generated using QuickChange XL II site - directed mutagenesis kit (Stratagene) and subcloned into the pMX - GFP (for JAK1) or pMEGIX - GFP (for JAK2) biscistronic retroviral vector upstream of the IRES as previously described.17 — 19 The mutated JAK1 and JAK2 constructs were verified by sequencing the full - length JAK1 and JAK2 using DYEnamic ET Dye Terminator Kit (Amersham Biosciences).